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Induction of secondary cell wall biosynthesis genes and their regulators by melatonin in Glycine max

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Abstract

Plant lodging severely reduced crop yield and quality. Different plant growth regulators (PGRs) have been applied to improve lodging resistance through the regulation of physiological changes, especially on the increase of stem thickness and strength. Melatonin is a pleiotropic PGR for the regulation of plant growth and development. In this study, we demonstrated that the exogenous treatment of melatonin to Glycine max significantly enhanced plant lateral growth by increasing stem diameter. In addition to the stem thickness, secondary cell wall (SCW) deposition acts as another critical factor for stem rigidity for lodging resistance. To understand whether exogenous treatment of melatonin would regulate SCW biosynthesis genes, we performed transcriptomic analyses on the stems of Glycine max with or without melatonin treatment. Through the differentially-expressed-genes (DEGs) analyses, many SCW biosynthesis genes were found to be regulated by melatonin, including the cellulose, hemicellulose and lignin biosynthesis enzymes. We also found that the two known master regulators, NAC and MYB, of SCW biosynthesis genes were induced under melatonin treatment, which further supported our observation on the differential expression of SCW biosynthesis genes. Our study highlighted the improvement of lodging resistance by the exogenous treatment of melatonin through the increase of plant stem thickness and the regulation of SCW biosynthesis genes and their upstream TFs in Glycine max.

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Funding

This work has received research support from CH Biotech R&D Co., LTD., in Taiwan, Nantou.

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Authors and Affiliations

  1. Department of Life Sciences, Institute of Plant Biology, College of Life Science, National Taiwan University, Taipei, Taiwan

    Chung-Ting Kao, Jia-Cheng Lin, Tsai-Pin Chou, Chang-Hung Chen, Jhong-He Yu & Ying-Chung Jimmy Lin

  2. CH Biotech R&D Co., LTD, Nantou, Taiwan

    Shang-Che Kuo & Ying-Chung Jimmy Lin

  3. Genome and Systems Biology Degree Program, National Taiwan University and Academia Sinica, Taipei, Taiwan

    Ya-Wen Huang, Cho-Chun Huang, Gui-Jun Li & Kai Xia

  4. Department of Biotechnology and Bioindustry Sciences, College of Bioscience and Biotechnology, National Cheng Kung University, Tainan, Taiwan

    Ying-Lan Chen

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  1. Chung-Ting Kao
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Contributions

Ying-Chung Jimmy Lin, Kai Xia, Cho-Chun Huang, Gui-Jun Li and Chung-Ting Kao contributed to the experimental design. The plant material preparation and melatonin treatment were performed by Ya-Wen Huang. The RNA extraction and cDNA library construction were performed by Chung-Ting Kao and Jhong-He Yu. DEGs analysis was performed by Chung-Ting Kao, Chang-Hung Chen and Shang-Che Kuo. qPCR was performed by Jia-Cheng Lin. The manuscript was written by Ying-Chung Jimmy Lin, Kai Xia, Ying-Lan Chen, Chung-Ting Kao and Tsai Pin Chou. All authors read and approved the final manuscript.

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Correspondence to Kai Xia or Ying-Chung Jimmy Lin.

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Kao, CT., Huang, YW., Lin, JC. et al. Induction of secondary cell wall biosynthesis genes and their regulators by melatonin in Glycine max. Plant Growth Regul (2024). https://doi.org/10.1007/s10725-024-01140-2

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