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Reduction of organic and inorganic selenium compounds by the edible medicinal basidiomycete Lentinula edodes and the accumulation of elemental selenium nanoparticles in its mycelium

  • Microbial Physiology and Biochemistry
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Abstract

We report for the first time that the medicinal basidiomycete Lentinula edodes can reduce selenium from inorganic sodium selenite (SeIV) and the organoselenium compound 1,5-diphenyl-3-selenopentanedione-1,5 (DAPS-25) to the elemental state, forming spherical nanoparticles. Submerged cultivation of the fungus with sodium selenite or with DAPS-25 produced an intense red coloration of L. edodes mycelial hyphae, indicating accumulation of elemental selenium (Se0) in a red modification. Several methods, including transmission electron microscopy (TEM), electron energy loss spectroscopy (EELS), and X-ray fluorescence, were used to show that red Se0 accumulated intracellularly in the fungal hyphae as electron-dense nanoparticles with a diameter of 180.51±16.82 nm. Under designated cultivation conditions, shiitake did not reduce selenium from sodium selenate (SeVI).

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Authors and Affiliations

  1. Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, Saratov, 410049, Russian Federation

    Elena Vetchinkina, Ekaterina Loshchinina, Viktor Kursky & Valentina Nikitina

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  1. Elena Vetchinkina
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  2. Ekaterina Loshchinina
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  3. Viktor Kursky
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  4. Valentina Nikitina
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Correspondence to Ekaterina Loshchinina.

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Vetchinkina, E., Loshchinina, E., Kursky, V. et al. Reduction of organic and inorganic selenium compounds by the edible medicinal basidiomycete Lentinula edodes and the accumulation of elemental selenium nanoparticles in its mycelium. J Microbiol. 51, 829–835 (2013). https://doi.org/10.1007/s12275-013-2689-5

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