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Overexpression of Neurospora crassa OR74A glutamate decarboxylase in Escherichia coli for efficient GABA production

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Abstract

This study investigated the effect of glutamate decarboxylase from Neurospora crassa OR74A on GABA production in Escherichia coli. GABA is one of the inhibitory neurotransmitters in the mammalian central nervous system, and can be used as a precursor of promising biopolymer Nylon 4. E. coli that overexpressed N. crassa glutamate decarboxylase was cultured at various pH levels and temperatures to determine optimum conditions for GABA production. When the recombinant E. coli strain was cultured at 30°C and pH 3, a final GABA concentration of 5.26 g/L was obtained from 10 g/L of monosodium glutamate (MSG), corresponding to a GABA yield of 86.23%.

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Ulsan, Ulsan, 680-749, Korea

    Tam Dinh Le Vo, Ji-seun Ko & Soon Ho Hong

  2. Industrial Biochemicals Research Group, Research Center for Biobased Chemistry, Division of Convergence Chemistry, Korea Research Institute of Chemical Technology, Daejeon, 305-600, Korea

    Seung Hwan Lee

  3. Department of Environmental Engineering and Energy, Myongji University, Yongin, 449-728, Korea

    Si Jae Park

Authors
  1. Tam Dinh Le Vo
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  2. Ji-seun Ko
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  3. Seung Hwan Lee
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  4. Si Jae Park
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  5. Soon Ho Hong
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Correspondence to Si Jae Park or Soon Ho Hong.

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Le Vo, T.D., Ko, Js., Lee, S.H. et al. Overexpression of Neurospora crassa OR74A glutamate decarboxylase in Escherichia coli for efficient GABA production. Biotechnol Bioproc E 18, 1062–1066 (2013). https://doi.org/10.1007/s12257-013-0282-8

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  • DOI: https://doi.org/10.1007/s12257-013-0282-8

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