Abstract
Gamma-aminobutyric acid (GABA) is an important bio-product, which is used in pharmaceutical formulations, nutritional supplements, and biopolymer monomer. The traditional GABA process involves the decarboxylation of glutamate. However, the direct production of GABA from glucose is a more efficient process. To construct the recombinant strains of Escherichia coli, a novel synthetic scaffold was introduced. By carrying out the co-localization of glutamate synthase, glutamate decarboxylase, and GABA transporter, we redirected the TCA cycle flux to GABA pathway. The genetically engineered E. coli strain produced 1.08 g/L of GABA from 10 g/L of initial glucose. Thus, with the introduction of a synthetic scaffold, we increased GABA production by 2.2-fold. The final GABA concentration was increased by 21.8 % by inactivating competing pathways.
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This work was financially supported by grants from the Next-Generation BioGreen 21 Program (SSAC, Grant Number: PJ01111601), Rural Development Administration, Republic of Korea.
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Dung Pham, V., Somasundaram, S., Lee, S.H. et al. Efficient production of gamma-aminobutyric acid using Escherichia coli by co-localization of glutamate synthase, glutamate decarboxylase, and GABA transporter. J Ind Microbiol Biotechnol 43, 79–86 (2016). https://doi.org/10.1007/s10295-015-1712-8
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DOI: https://doi.org/10.1007/s10295-015-1712-8