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Effects of glutamate decarboxylase and gamma-aminobutyric acid (GABA) transporter on the bioconversion of GABA in engineered Escherichia coli

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Abstract

Gamma-aminobutyric acid (GABA) is a non-essential amino acid and a precursor of pyrrolidone, a monomer of nylon 4. GABA can be biosynthesized through the decarboxylation of l-glutamate by glutamate decarboxylase. In this study, the effects of glutamate decarboxylase (gadA, gadB), glutamate/GABA antiporter (gadC) and GABA aminotransferase (gabT) on GABA production were investigated in Escherichia coli. Glutamate decarboxylase was overexpressed alone or with the glutamate/GABA antiporter to enhance GABA synthesis. GABA aminotransferase, which redirects GABA into the TCA cycle, was knock-out mutated. When gadB and gadC were co-overexpressed in the gabT mutant strain, a final GABA concentration of 5.46 g/l was obtained from 10 g/l of monosodium glutamate (MSG), which corresponded to a GABA yield of 89.5%.

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Acknowledgments

This work was supported by the R&D Program of MKE/KEIT (10033199) and the Next-Generation BioGreen 21 Program (SSAC, Grant number: PJ008057), Rural Development Administration, Republic of Korea.

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Authors and Affiliations

  1. School of Chemical Engineering and Bioengineering, University of Ulsan, 93 Daehak-ro, Nam-gu, Ulsan, 680-749, Korea

    Tam Dinh Le Vo & Soon Ho Hong

  2. Marine Biotechnology Research Center, Korea Ocean Research and Development Institute, PO Box 29, Ansan, 425-600, Korea

    Tae Wan Kim

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  1. Tam Dinh Le Vo
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  2. Tae Wan Kim
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Correspondence to Soon Ho Hong.

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Le Vo, T.D., Kim, T.W. & Hong, S.H. Effects of glutamate decarboxylase and gamma-aminobutyric acid (GABA) transporter on the bioconversion of GABA in engineered Escherichia coli . Bioprocess Biosyst Eng 35, 645–650 (2012). https://doi.org/10.1007/s00449-011-0634-8

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  • DOI: https://doi.org/10.1007/s00449-011-0634-8

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