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Studies on varietal response to different strains of Potato virus Y (PVY) reveal hypersensitive resistance in Exploits to PVYO and extreme resistance in F87084 to all tested strains

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Abstract

Potato cultivar Exploits and breeding clone F87084 have been considered resistant to Potato virus Y (PVY). To further explore the degree of resistance and whether the resistance is strain specific, these materials, together with cultivar Rochdale Gold-Dorée and breeding line F02010, were investigated for their response to different PVY strains including PVYO, PVYN:O, PVYNTN and PVYN. Both F02010 and Rochdale Gold-Dorée were readily infected with all tested PVY strains after either mechanical or graft inoculation, indicating susceptibility of the materials to PVY. F87084 was unable to be infected by any of the tested PVY strains as no ELISA-detectable level of PVY were found in plants after either mechanical or graft inoculation, demonstrating extreme resistance in F87084 to all strains of PVY tested. Exploits was infected with PVYN:O/PVYNTN/PVYN after either mechanical or graft inoculation, indicating susceptibility of the cultivar to these PVY strains. The cultivar was also infected readily with PVYO after graft inoculation. However, despite induction of local lesions on the inoculated leaves, mechanical inoculation with PVYO may or may not lead to systemic symptoms and ELISA-detectable level of PVY, depending on temperature. At low temperature (e.g., 22 °C), no visible systemic symptoms or ELISA-detectable level of PVY was found in the plants; whereas at high temperature (e.g., 30 °C), systemic symptoms and high level of PVY were detected in the plants. These results demonstrate that Exploits possesses temperature-dependent hypersensitive resistance to PVYO. Analysis of a segregating population of F87084 × F02010 revealed that the ER in F87084 is controlled by a dominant resistance gene.

Resumen

La variedad de papa Exploits y el clon avanzado F87084 han sido considerados como resistentes al virus Y de la papa (PVY). Para una mayor exploración sobre el grado de resistencia y determinar si la resistencia es específica por variante viral, estos materiales, junto con la variedad Rochdale Gold-Dorée y la línea avanzada F02010, se investigaron para su respuesta a diferentes variantes del PVY, incluyendo PVYO, PVYN:O, PVYNTN y PVYN. Tanto F02010 como Rochdale Gold-Dorée fueron fácilmente infectadas con todas las variantes de prueba después de inoculación mecánica o por injerto, indicando susceptibilidad de los materiales al PVY. No fue posible infectar al clon F87084 por cualquiera de las variantes probadas de PVY y tampoco se encontró nivel detectable de PVY mediante ELISA en las plantas después de inoculación mecánica o por injerto, demostrando resistencia extrema en F87084 a todas las variantes probadas de PVY. Se infectó a Exploits con PVYN:O/PVYNTN/PVYN después de inoculación mecánica o injerto, indicando susceptibilidad de la variedad a estas variantes del PVY. La variedad también fue fácilmente infectada con PVYO después de la inoculación por injerto. No obstante, a pesar de la inducción de lesiones locales en las hojas inoculadas, la inoculación mecánica con PVY puede o no conducir a síntomas sistémicos y a nivel de PVY detectable por ELISA, dependiendo de la temperatura. A baja temperatura (ej. 22ºC) no se encontraron síntomas sistémicos visibles o niveles de PVY detectables por ELISA en las plantas; mientras que a alta temperatura (ej. 30ºC), se detectaron síntomas sistémicos y alto nivel de PVY. Estos resultados demuestran que Exploits tiene resistencia hipersensible al PVYO dependiente de la temperatura. Un análisis de la población segregante de F87084 x F02010 reveló que la ER en F87084 es controlada por un gene dominante de resistencia.

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Acknowledgments

The authors thank Virginia Dickison, Donna Wilson, Catherine Desjardins, and Angela Gallagher for technical assistance; the greenhouse staff at the Potato Research Centre for horticultural assistance; Dr. David De Koeyer for helpful discussion. This research was funded by Agriculture and Agri-Food Canada under the Peer-Reviewed Projects #1789 and #1110.

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Authors and Affiliations

  1. Potato Research Centre, Agriculture and Agri-Food Canada, P.O. Box 20280, 850 Lincoln Road, Fredericton, NB, Canada, E3B 4Z7

    Xianzhou Nie, Zengenni Liang, Bihua Nie & Agnes Murphy

  2. College of Horticulture and Landscape, Hunan Agricultural University, Changsha, Hunan, China, 410128

    Zengenni Liang

  3. National Center for Vegetable Improvement (Central China), MOE Key Laboratory of Horticultural Plant Biology, Hubei Provincial Research Center of Potato Engineering and Technology, Huazhong Agricultural University, Wuhan, China, 430070

    Bihua Nie

  4. Agricultural Certification Services, 1030 Lincoln Road Fredericton, New Brunswick, Canada, E3B8B7

    Mathuresh Singh

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  1. Xianzhou Nie
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  3. Bihua Nie
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Correspondence to Xianzhou Nie.

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Supplementary Figure 1

Response of potato line F02010 to graft/mechanical inoculation with different strains of Potato virus Y (PVY). a Mechanically inoculated leaves. b Mechanically inoculated plants. c Graft-inoculated plants. Photos were taken 7 days post inoculation (dpi) for a and 14 dpi for b and c. ELISA for each plant was carried out at 18 dpi using a mix of leaf pieces of three leaves that emerged after inoculation. The absorbance at 405 nm (A405) shown below each treatment is mean ± standard deviation of 4 plants (replicates). The experiments were repeated two times and similar results were obtained. The ELISA results were from first repeat (JPEG 859 kb)

Supplementary Figure. 2

Response of potato cultivar Rochdale Gold-Dorée to graft/mechanical inoculation with different strains of Potato virus Y (PVY). a Mechanically inoculated leaves. b Mechanically inoculated plants. c Graft-inoculated plants. Photos were taken 7 days post inoculation (dpi) for a and 14 dpi for b and c ELISA for each plant was carried out at 18 dpi using a mix of leaf pieces of three leaves that emerged after inoculation. The absorbance at 405 nm (A405) shown below each treatment is mean ± standard deviation of 4 plants (replicates). The experiments were repeated two times and similar results were obtained. The ELISA results were from first repeat (JPEG 844 kb)

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Nie, X., Liang, Z., Nie, B. et al. Studies on varietal response to different strains of Potato virus Y (PVY) reveal hypersensitive resistance in Exploits to PVYO and extreme resistance in F87084 to all tested strains. Am. J. Potato Res. 92, 23–31 (2015). https://doi.org/10.1007/s12230-014-9407-7

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