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Tracking the R Mc1 Gene for Resistance to Race 1 of Columbia Root-Knot Nematode (Meloidogyne chitwoodi) in Three Mexican Wild Potato Species with Different Ploidies

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Abstract

Resistance to Columbia Root-knot nematode was discovered in three wild species in a polyploidy series, Solanum bulbocastanum (2n = 2x), .S. fendleri (2n = 4x), and S. hougasii, 2n = 6x, respectively. After several generations of introgression into the cultivated gene pool, a single dominant monogene conditioning resistance to root propagation of race 1 of CRKN was theorized to be acting in breeding lines derived from each species. Coincidently, sequence tagged sites (STS) and cleaved amplified polymorphic site (CAPS) markers that associated with resistance with S. bulbocastanum were found to be informative in other segregating breeding populations regardless of the source of the gene. The utility of the markers across ploidies suggests that the original gene and genome of S. bulbocastanum or a modern derivative, is present in S. fendleri and S. hougasii. In this polyploidy series, knowledge that the S. bulbocastanum genome is the original source of useful genes guides future germplasm surveyors to search first and most intensively in the primitive Mexican diploid species that may have served as the contributor of a genome to descendants harboring this genome at higher ploidies.

Resumen

Se descubrió la resistencia al nematodo agallador de Columbia (CRKN) en tres especies silvestres en una serie de poliploidia, Solanum bulbocastanum (2n = 2x), .S. fendleri (2n = 4x), y S. hougasii, 2n = 6x, respectivamente. Después de varias generaciones de introgresión hacia el grupo de genes cultivados, se teorizó que un monogene dominante sencillo condiciona la resistencia a la propagación de raíz de la raza 1 de CRKN actuando en líneas de mejoramiento derivadas de cada especie. Coincidentemente, secuencias etiquetadas de sitios (STS) y marcadores de un sitio polimórfico amplificado (CAPS) que se asociaron con la resistencia con S. bulbocastanum, resultaron ser informativos en otras poblaciones segregantes de mejoramiento, sin importar la fuente del gen. La utilidad de estos marcadores a lo largo de las ploidias sugiere que el gen original y el genoma de S. bulbocastanum o de un derivado moderno, está presente en S. fendleri y S. hougasii. En esta serie de ploidias el saber que el genomio de S. bulbocastanum es la fuente original de genes útiles, conduce a los buscadores de futuro germoplasma a investigar primero y más intensamente en las especies diploides primitivas mexicanas que pudieran haber servido como contribuyentes de genomio a descendientes, protegiendo este genomio para ploidias mayores.

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Authors and Affiliations

  1. USDA/ARS, Washington, DC, USA

    Charles R. Brown

  2. Irrigated Agriculture Research and Extension Center, Washington State University, 24106 N. Bunn Rd., Prosser, WA, USA

    Linhai Zhang & Hassan Mojtahedi

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  1. Charles R. Brown
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  2. Linhai Zhang
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  3. Hassan Mojtahedi
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Correspondence to Charles R. Brown.

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Brown, C.R., Zhang, L. & Mojtahedi, H. Tracking the R Mc1 Gene for Resistance to Race 1 of Columbia Root-Knot Nematode (Meloidogyne chitwoodi) in Three Mexican Wild Potato Species with Different Ploidies. Am. J. Potato Res. 91, 180–185 (2014). https://doi.org/10.1007/s12230-013-9336-x

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  • DOI: https://doi.org/10.1007/s12230-013-9336-x

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