Abstract
Twelve accessions ofSolarium fendleri collected in the United States and Mexico were inoculated with 5,000 eggs of host-races 1 and 2 ofMeloidogyne chitwoodi, Columbia root-knot nematode. The test seedlings that were derived from plant introduction true seed lots were maintained for 55 days before harvest and egg count. The results revealed that two accessions, PI 275162 and PI 275165, were non-hosts (final egg count/initial egg inoculation < 0.1) forM. chitwoodi race 1. The results were uniformly confirmed in the second experiment. No resistance was found to race 2. The tetraploidS. fendleri accessions were crossed to a nematode-susceptible cultivated diploid potato clone from aS. phurejastenotomum population. The triploid hybrids expressed resistance to race 1 at the non-host level. After somatic doubling, the resulting hexaploids also expressed non-host-level resistance to race 1. The two resistant accessions had been collected in southeastern Arizona, one each from the Huachuca and Chiricahua Mountains. Each range is an isolated island of high-elevation mesic flora surrounded by typical lowerelevation Sonora-type desert habitat. These accessions are the only known sources of resistance toM. chitwoodi from wildSolanum species in the USA.
Resumen
Doce accesiones deSolanum fendleri colectadas en los Estados Unidos y México se inocularon con 5,000 huevos de las razas 1 y 2 deMeloidogyne chitwoodi, nematodo del nudo de Columbia. Las plántulas en prueba, derivadas de una introducción de semilla botanica fueron mantenidas por 55 dias antes de cosecharlas y contar los huevos. Los resultados revelaron que dos de las accesiones, PI 275162 y PI 275165 no son hospedantes (conteo final de huevos / inoculación inicial de huevos < 0.1) para la raza 1 deM. chitwoodi. Los resultados fueron fielmente confirmados en el segundo experimento No se encontró resistencia a la raza 2. Las accesiones tetraploides deS. fendleri fueron cruzadas con un clon de papa cultivada diploide deS. phurejastenotomum de una población susceptible al nematodo. Los híbridos triploides expresaron resistencia a la raza 1 en el nivel de no-hospedante. Después de la duplicación somática, el hexaploide résultante también expresó resistencia a la raza 1 en el nivel de no-hospedante. Las dos accesiones resistentes han sido colectadas en el sudeste de Arizona, una en Huachuca y otra en Chiricahua Mountains. Cada monte es una isla aislada con flora de humedad media, rodeada de una elevación más baja del tipico habitat desértico de Sonora. Estas accesiones son la única fuente conocida de resistencia alM. chitwoodi de las especies silvestres de los EUA.
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Brown, C.R., Mojtahedi, H. & Bamberg, J. Evaluation ofSolarium fendleri as a source of resistance toMeloidogyne chitwoodi . Am. J. Pot Res 81, 415–419 (2004). https://doi.org/10.1007/BF02870202
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DOI: https://doi.org/10.1007/BF02870202