Abstract
Callus cell cultures of Arabidopsis thaliana exposed to hypergravity (8×g), 2D clinorotation and random positioning exhibit changes in gene expression (Martzivanou et al., Protoplasma 229:155–162, 2006). In a recent investigation we could show that after 2 h of exposure also the protein complement shows treatment-related changes which are indicative for reactive oxygen species being involved in the perception of/response to changes in the gravitational field. In the present study we have extended these investigations for a period of up to 16 h of exposure. We report on changes in abundance of 28 proteins which have been identified by nano HPLC-ESI-MS/MS, and which were altered in amount after 2 h of treatment. According to changes between 2 and 16 h we could distinguish four groups of proteins which either declined, increased from down-regulated to control levels, showed a transient decline or a transient increase. With regard to function, our data indicate stress relief or adaptation to a new gravitational steady state under prolonged exposure. The latter assumption is supported by the appearance of a new set of 19 proteins which is changed in abundance after 8 h of hypergravity. A comparative analysis of the different treatments showed some similarities in response between 8×g centrifugation and 2D clinorotation, while random positioning showed the least responses.
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Babbick, M., Dijkstra, C., Larkin, O.J., Anthony, P., Davey, M.R., Power, J.B., Lowe, K.C., Cogoli-Greuter, M., Hampp, R.: Expression of transcription factors after short-term exposure of Arabidopsis thaliana cell cultures to hypergravity and simulated microgravity (2-D/3-D clinorotation, magnetic levitation). Adv. Space Res. 39, 1182–1189 (2006)
Barjaktarović, Z., Babbick, M., Lamkemeyer, T., Fladerer, C., Madlung, J., Hampp, R.: Time course of changes in protein amounts of specific proteins upon exposure to hyper-g, 2-D clinorotation and random positioning of Arabidopsis cell cultures. J. Exp. Bot. 58(15–16), 4357–4363 (2007)
Centis-Aubay, S., Gasset, G., Mazars, C., Ranjeva, R., Graziana, A.: Changes in gravitational forces induce modifications of gene expression in A. thaliana seedlings. Planta 218, 179–185 (2003)
Hampp, R., Hoffmann, E., Schönherr, K., Johann, P., Filippis, L.D.: Fusion and metabolism of plant cells as affected by microgravity. Planta 203, S42–53 (1997)
Kimbrough, J.M., Salinas-Mondragon, R., Boss, W.F., Brown, C.S., Winter Sederoff, H.: The fast and transient transcriptional network of gravity and mechanical stimulation in the Arabidopsis root apex. Plant Physiol. 136, 2790–2805 (2004)
Laskowski, M.J., Dreher, K.A., Gehring, M.A., Abel, S., Gensler, A.L., Sussex, I.M.: FQR1, a novel primary auxin-response gene, encodes a flavin mononucleotide-binding quinone reductase. Plant Physiol. 128(2), 578–590 (2007)
Martzivanou, M., Hampp, R.: Hyper-gravity effects on the Arabidopsis transcriptome. Physiol. Plant 118, 221–231 (2003)
Martzivanou, M., Babbick, M., Cogoli-Greuter, M., Hampp, R.: Microgravity-related changes in gene expression after short-term exposure of Arabidopsis thaliana cell cultures. Protoplasma 229(2–4), 155–162 (2006)
Moeder, W., Del Pozo, O., Navarre, D.A., Martin, G.B., Klessig, D.F.: Aconitase plays a role in regulating resistance to oxidative stress and cell death in Arabidopsis and Nicotiana benthamiana. Plant Mol. Biol. 63(2), 273–287 (2007)
Moseyko, N., Zhu, T., Chang, H.S., Wang, X., Feldman, L.J.: Transcription profiling of the early gravitropic response in Arabidopsis using high-density oligonucleotide probe microarrays. Plant Physiol. 130, 720–728 (2002)
Niini, S.S., Tarkka, M.T., Raudaskoski, M.: Tubulin and actin protein patterns in Scots pine (Pinus sylvestris) roots and developing ectomycorrhiza with Suillus bovinus. Physiol. Plant 96, 186–192 (1996)
Perkins, D.N., Pappin, D.J., Creasy, D.M., Cottrell, J.S.: Probability-based protein identification by searching sequence databases using mass spectrometry data. Electrophoresis 20, 3551–3567 (1999)
Resch, A., Leicht, S., Saric, M., Pásztor, L., Jakob, A., Götz, F., Nordheim, A.: Comparative proteome analysis of Staphylococcus aureus biofilm and planktonic cells and correlation with transcriptome profiling. Proteomics 6, 1867–1877 (2006)
Soga, K., Wakabayashi, K., Kamisaka, S., Hoson, T.: Effects of hypergravity on expression of XTH genes in azuki bean epicotyls. Physiol. Plant 131, 332–340 (2007)
Walther, I., Pippia, P., Meloni, M.A., Turrini, F., Mannu, F., Cogoli, A.: Simulated microgravity inhibits the genetic expression of interleukin-2 and its receptor in mitogen-activated T lymphocytes. FEBS Lett. 436, 115–118 (1998)
Wang, H., Zheng, H.Q., Sha, W., Zeng, R., Xia, Q.C.: A proteomic approach to analysing responses of Arabidopsis thaliana callus cells to clinostat rotation. J. Exp. Bot. 57, 827–835 (2006)
Yoshioka, R., Soga, K., Wakabayashi, K., Takeba, G., Hoson, T.: Hypergravity-induced changes in gene expression in Arabidopsis hypocotyls. Adv. Space Res. 31, 2187–2193 (2003)
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Barjaktarović, Ž., Babbick, M., Nordheim, A. et al. Alterations in Protein Expression of Arabidopsis thaliana Cell Cultures During Hyper- and Simulated Micro-Gravity. Microgravity Sci. Technol 21, 191–196 (2009). https://doi.org/10.1007/s12217-008-9058-8
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DOI: https://doi.org/10.1007/s12217-008-9058-8