Abstract
Plant tissue culture techniques are the most frequently used biotechnological tools for basic and applied purposes ranging from investigation on plant developmental processes, functional gene studies, commercial plant micropropagation, generation of transgenic plants with specific industrial and agronomical traits, plant breeding and crop improvement, virus elimination from infected materials to render high-quality healthy plant material, preservation and conservation of germplasm of vegetative propagated plant crops, and rescue of threatened or endangered plant species. Additionally, plant cell and organ cultures are of interest for the production of secondary metabolites of industrial and pharmaceutical interest. New technologies, such as the genome editing ones combined with tissue culture and Agrobacterium tumefaciens infection, are currently promising alternatives for the highly specific genetic manipulation of interesting agronomical or industrial traits in crop plants. Application of omics (genomics, transcriptomics, and proteomics) to plant tissue culture will certainly help to unravel complex developmental processes such as organogenesis and somatic embryogenesis, which will probably enable to improve the efficiency of regeneration protocols for recalcitrant species. Additionally, metabolomics applied to tissue culture will facilitate the extraction and characterization of complex mixtures of natural plant products of industrial interest. General and specific aspects and applications of plant tissue culture and the advances and perspectives are described in this edition.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Haberlandt G (1902) Kulturversuche mit isolierten pflanzenzellen. Sber Akad Wiss Wein 111:69–92
White PR (1934) Potentially unlimited growth of excised tomato root tips in a liquid medium. Plant Physiol 9:585–600. https://doi.org/10.1104/pp.9.3.585
White PR (1939) Controlled differentiation in a plant tissue culture. Bull Torrey Bot Club 66:507–513
White PR (1939) Potentially unlimited growth of excised plant callus in an artificial nutrient. Am J Bot 26:59–64
Knudson L (1922) Nonsymbiotic germination of orchid seeds. Bot Gaz 73:1–25. https://doi.org/10.1086/332956
Thimann KV, Schneider CL (1939) The relative activities of different auxins. Am J Bot 26:328–333
Miller CO, Skoog F, Von Saltza MH et al (1955) Kinetin, a cell division factor from deoxyribonucleic acid. J Am Chem Soc 77:1392. https://doi.org/10.1021/ja01610a105
Skoog F, Miller CO (1957) Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp Soc Exp Biol 11:118–131
Morel G, Martin G (1952) Guérison de dahlías atteints d’une maladie a virus. CR Acad Sci III-Vie 235:1324–1325
Guha S, Maheshwari SC (1966) Cell division and differentiation of embryos in the pollen grain of Datura in vitro. Nature 212:97–98. https://doi.org/10.1038/212097a0
Guha S, Maheshwari SC (1964) In vitro production of embryos from anthers of Datura. Nature 204:497. https://doi.org/10.1038/204497a0
Raghavan V (2003) One hundred years of zygotic embryo culture investigations. In Vitro Cell Dev Biol Plant 39:437–442. https://doi.org/10.1079/IVP2003436
Power JB, Cummind SE, Cocking EC (1970) Fusion of isolated protoplasts. Nature 225:1016–1018. https://doi.org/10.1038/2251016a0
Melchers G, Sacristán MD, Holder AA (1978) Somatic hybrid plants of potato and tomato regenerated from fused protoplasts. Carlsb Res Commun 43:203–218. https://doi.org/10.1007/BF02906548
Zenk MH (1991) Chasing the enzymes of secondary metabolism: plant cell cultures as a pot of gold. Phytochemistry 30:3861–3863. https://doi.org/10.1016/0031-9422(91)83424-J
Fraley RT, Rogers SG, Horsch RB et al (1983) Expression of bacterial genes in plant cells. Proc Natl Acad Sci U S A 80:4803–4807
Larkin PJ, Scowcroft WR (1981) Somaclonal variation -a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60:197–214. https://doi.org/10.1007/BF02342540
Lestari EG (2006) In vitro selection and somaclonal variation for biotic and abiotic stress tolerance. Biodiversitas 7:297–301
Lotfi M, Alan AR, Henning MJ et al (2003) Production of haploid and doubled haploid plants of melon (Cucumis melo L.) for use in breeding for multiple virus resistance. Plant Cell Rep 21:1121–1128. https://doi.org/10.1007/s00299-003-0636-3
Germanà MA (2011) Anther culture for haploid and doubled haploid production. Plant Cell Tissue Org 104:283–300. https://doi.org/10.1007/s11240-010-9852-z
Stewart JM (1981) In vitro fertilization and embryo rescue. Environ Exp Bot 21:301–315. https://doi.org/10.1016/0098-8472(81)90040-X
Ji W, Li GR, Luo YX et al (2015) In vitro embryo rescue culture of F1 progenies from crosses between different ploidy grapes. Genet Mol Res 14:18616–18622
Borgato L, Conicella C, Pisani F et al (2007) Production and characterization of arboreous and fertile Solanum melongena + Solanum marginatum somatic hybrid plants. Planta 226:961–969. https://doi.org/10.1007/s00425-007-0542-y
Gx W, Tang Y, Yan H et al (2011) Production and characterization of interspecific somatic hybrids between Brassica oleracea var. botrytis and B. nigra and their progenies for the selection of advanced pre-breeding materials. Plant Cell Rep 30:1811–1821. https://doi.org/10.1007/s00299-011-1088-9
Prange ANS, Bartsch M, Meiners J et al (2012) Interspecific somatic hybrids between Cyclamen persicum and C. coum, two sexually incompatible species. Plant Cell Rep 31:723–735. https://doi.org/10.1007/s00299-011-1190-z
Yu Y, Ye W, He L et al (2013) Introgression of bacterial wilt resistance from eggplant to potato via protoplast fusion and genome components of the hybrids. Plant Cell Rep 32:1687–1701. https://doi.org/10.1007/s00299-013-1480-8
Liu S, Xia G (2014) The place of asymmetric somatic hybridization in wheat breeding. Plant Cell Rep 33:595–603. https://doi.org/10.1007/s00299-013-1552-9
Gatehouse JA (2008) Biotechnological prospects for engineering insect-resistant plants. Plant Physiol 146:881–887. https://doi.org/10.1104/pp.107.111096
Green JM, Owen MDK (2011) Herbicide-resistant crops: utilities and limitations for herbicide-resistant weed management. J Agric Food Chem 59:5819–5829. https://doi.org/10.1021/jf101286h
Zhang HX, Blumwald E (2001) Transgenic salt-tolerant tomato plants accumulate salt in foliage but not in fruit. Nat Biotechnol 19:765. https://doi.org/10.1038/90824
Hu H, Dai M, Yao J et al (2006) Overexpressing a NAM, ATAF, and CUC (NAC) transcription factor enhances drought resistance and salt tolerance in rice. Proc Natl Acad Sci U S A 103:12987–12992. https://doi.org/10.1073/pnas.0604882103
Rivero RM, Kojima M, Gepstein A et al (2007) Delayed leaf senescence induces extreme drought tolerance in a flowering plant. Proc Natl Acad Sci U S A 104:19631–19636. https://doi.org/10.1073/pnas.0709453104
Todaka D, Shinozaki K, Yamaguchi-Shinozaki K (2015) Recent advances in the dissection of drought-stress regulatory networks and strategies for development of drought-tolerant transgenic rice plants. Front Plant Sci 6:84. https://doi.org/10.3389/fpls.2015.00084
Sanghera GS, Wani SH, Hussain W et al (2011) Engineering cold stress tolerance in crop plants. Curr Genomics 12:30–43. https://doi.org/10.2174/138920211794520178
Cardi T, Neal Stewart C (2016) Progress of targeted genome modification approaches in higher plants. Plant Cell Rep 35:1401–1416. https://doi.org/10.1007/s00299-016-1975-1
Subburaj S, Tu L, Jin YT et al (2016) Targeted genome editing, an alternative tool for trait improvement in horticultural crops. Hortic Environ Biotechnol 57:531–543. https://doi.org/10.1007/s13580-016-0281-8
Belhaj K, Chaparro-Garcia A, Kamoun S et al (2015) Editing plant genomes with CRISPR/Cas9. Curr Opin Biotechnol 32:76–84. https://doi.org/10.1016/j.copbio.2014.11.007
Luo M, Gilbert B, Ayliffe M (2016) Applications of CRISPR/Cas9 technology for targeted mutagenesis, gene replacement and stacking of genes in higher plants. Plant Cell Rep 35:1439–1450. https://doi.org/10.1007/s00299-016-1989-8
Mahfouz MM, Cardi T, Neal Stewart C (2016) Next-generation precision genome engineering and plant biotechnology. Plant Cell Rep 35:1397–1399. https://doi.org/10.1007/s00299-016-2009-8
Kanchiswamy CN (2016) DNA-free genome editing methods for targeted crop improvement. Plant Cell Rep 35:1469–1474. https://doi.org/10.1007/s00299-016-1982-2
Li M, Li X, Zhou Z et al (2016) Reassessment of the four yield-related genes Gn1a, DEP1, GS3, and IPA1 in rice using a CRISPR/Cas9 system. Front Plant Sci 7:377. https://doi.org/10.3389/fpls.2016.00377
Srivastava V, Underwood JL, Zhao S (2017) Dual-targeting by CRISPR/Cas9 for precise excision of transgenes from rice genome. Plant Cell Tissue Org 129:153–160. https://doi.org/10.1007/s11240-016-1166-3
Wang Y, Cheng X, Shan Q et al (2014) Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew. Nat Biotechnol 32:947–951. https://doi.org/10.1038/nbt.2969
Feng C, Yuan J, Wang R et al (2016) Efficient targeted genome modification in maize using CRISPR/Cas9 system. J Genet Genomics 43:37–43. https://doi.org/10.1016/j.jgg.2015.10.002
Soyk S, Muller NA, Park SJ et al (2017) Variation in the flowering gene SELF PRUNING 5G promotes day-neutrality and early yield in tomato. Nat Genet 49:162–168. https://doi.org/10.1038/ng.3733
Wang S, Zhang S, Wang W et al (2015) Efficient targeted mutagenesis in potato by the CRISPR/Cas9 system. Plant Cell Rep 34:1473–1476. https://doi.org/10.1007/s00299-015-1816-7
Zhou JP, Deng K, Cheng Y et al (2017) CRISPR-Cas9 based genome editing reveals new insights into microRNA function and regulation in rice. Front Plant Sci 8:1598. https://doi.org/10.3389/fpls.2017.01598
Lowder LG, Malzahn A, Qi Y (2018) Plant gene regulation using multiplex CRISPR-dCas9 artificial transcription factors. In: Lagrimini LM (ed) Maize: methods and protocols. Springer, New York, pp 197–214. https://doi.org/10.1007/978-1-4939-7315-6_12
Neelakandan AK, Wang K (2012) Recent progress in the understanding of tissue culture-induced genome level changes in plants and potential applications. Plant Cell Rep 31:597–620. https://doi.org/10.1007/s00299-011-1202-z
Wickramasuriya AM, Dunwell JM (2015) Global scale transcriptome analysis of Arabidopsis embryogenesis in vitro. BMC Genomics 16:301. https://doi.org/10.1186/s12864-015-1504-6
Imin N, Nizamidin M, Daniher D et al (2005) Proteomic analysis of somatic embryogenesis in Medicago truncatula. Explant cultures grown under 6-benzylaminopurine and 1-naphthaleneacetic acid treatments. Plant Physiol 137:1250–1260. https://doi.org/10.1104/pp.104.055277
Turi CE, Axwik KE, Murch SJ (2014) In vitro conservation, phytochemistry, and medicinal activity of Artemisia tridentata Nutt.: metabolomics as a hypothesis-generating tool for plant tissue culture. Plant Growth Regul 74:239–250. https://doi.org/10.1007/s10725-014-9915-y
Vasilev N, Boccard J, Lang G et al (2016) Structured plant metabolomics for the simultaneous exploration of multiple factors. Sci Rep 6:37390. https://doi.org/10.1038/srep37390
Kaeppler SM, Kaeppler HF, Rhee Y (2000) Epigenetic aspects of somaclonal variation in plants. Plant Mol Biol 43:179–188. https://doi.org/10.1023/A:1006423110134
Nic-Can GI, De-la-Peña C (2014) Epigenetic advances on somatic embryogenesis of agronomical and important crops. In: Álvarez-Venegas R, De-la-Peña C, Casas-Mollano JA (eds) Epigenetics in plants of agronomic importance: fundamentals and applications. Springer, Cham, pp 91–109. https://doi.org/10.1007/978-3-319-07971-4_6
De-la-Peña C, Nic-Can GI, Galaz-Ávalos RM et al (2015) The role of chromatin modifications in somatic embryogenesis in plants. Front Plant Sci 6:635. https://doi.org/10.3389/fpls.2015.00635
Duarte-Aké F, Castillo-Castro E, Pool FB et al (2016) Physiological differences and changes in global DNA methylation levels in Agave angustifolia haw. Albino variant somaclones during the micropropagation process. Plant Cell Rep 25:2489–2502. https://doi.org/10.1007/s00299-016-2049-0
Duarte-Aké F, De-la-Peña C (2016) Epigenetic advances in somatic embryogenesis in sequenced genome crops. In: Loyola-Vargas VM, Ochoa-Alejo N (eds) Somatic embryogenesis: fundamental aspects and applications. Springer, Cham, pp 81–102. https://doi.org/10.1007/978-3-319-33705-0_6
Smulders M, de Klerk G (2011) Epigenetics in plant tissue culture. Plant Growth Regul 63:137–146. https://doi.org/10.1007/s10725-010-9531-4
Rival A, Ilbert P, Labeyrie A et al (2013) Variations in genomic DNA methylation during the long-term in vitro proliferation of oil palm embryogenic suspension cultures. Plant Cell Rep 32:359–368. https://doi.org/10.1007/s00299-012-1369-y
Us-Camas R, Rivera-Solís G, Duarte-Aké F et al (2014) In vitro culture: an epigenetic challenge for plants. Plant Cell Tissue Org 118:187–201. https://doi.org/10.1007/s11240-014-0482-8
Lau W, Fischbach MA, Osbourn A et al (2014) Key applications of plant metabolic engineering. PLoS Biol 12:e1001879. https://doi.org/10.1371/journal.pbio.1001879
Vanhercke T, El Tahchy A, Liu Q et al (2014) Metabolic engineering of biomass for high energy density: oilseed-like triacylglycerol yields from plant leaves. Plant Biotechnol J 12:231–239. https://doi.org/10.1111/pbi.12131
Lu X, Tang K, Li P (2016) Plant metabolic engineering strategies for the production of pharmaceutical terpenoids. Front Plant Sci 7:1647. https://doi.org/10.3389/fpls.2016.01647
Tatsis EC, O’Connor SE (2016) New developments in engineering plant metabolic pathways. Curr Opin Biotechnol 42:126–132. https://doi.org/10.1016/j.copbio.2016.04.012
Krishna H, Alizadeh M, Singh D et al (2016) Somaclonal variations and their applications in horticultural crops improvement. 3 Biotech 6:54. https://doi.org/10.1007/s13205-016-0389-7
Kim H, Kim S-T, Kim S-G et al (2015) Targeted genome editing for crop improvement. Plant Breed Biotechnol 3:283–290. https://doi.org/10.9787/PBB.2015.3.4.283
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Loyola-Vargas, V.M., Ochoa-Alejo, N. (2018). An Introduction to Plant Tissue Culture: Advances and Perspectives. In: Loyola-Vargas, V., Ochoa-Alejo, N. (eds) Plant Cell Culture Protocols. Methods in Molecular Biology, vol 1815. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-8594-4_1
Download citation
DOI: https://doi.org/10.1007/978-1-4939-8594-4_1
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-8593-7
Online ISBN: 978-1-4939-8594-4
eBook Packages: Springer Protocols