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Microgravity-related changes in gene expression after short-term exposure of Arabidopsis thaliana cell cultures

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Summary.

Cell cultures of Arabidopsis thaliana (cv. Columbia) were used to screen for early alterations in gene expression as a response to altered gravitational fields. Genes of interest were selected from a larger group whose expression was altered under hypergravity (microarray study; M. Martzivanou and R. Hampp, Physiol. Plant. 118: 221–231, 2003). Transcriptional changes of these genes were studied within a time frame of up to 10 min of exposure to microgravity in a sounding-rocket experiment, to clinorotation (random positioning machine), and to hypergravity (8 g). We could identify a set of nine genes (mainly components of signaling chains) with increased transcript levels after about 6 min of exposure to microgravity. As clinorotation and hypergravity treatment did not alter the respective transcript amounts, we assume that the identified genes could be involved in a microgravity-related response.

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Authors and Affiliations

  1. Physiological Ecology of Plants, University of Tübingen, Tübingen

    M. Martzivanou, M. Babbick & R. Hampp

  2. Space Biology Group, Swiss Federal Institute of Technology, Zürich

    M. Cogoli-Greuter

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  1. M. Martzivanou
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  2. M. Babbick
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  3. M. Cogoli-Greuter
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  4. R. Hampp
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Correspondence to R. Hampp.

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Correspondence and reprints: Physiological Ecology of Plants, University of Tübingen, Auf der Morgenstelle 1, 72076 Tübingen, Federal Republic of Germany.

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Martzivanou, M., Babbick, M., Cogoli-Greuter, M. et al. Microgravity-related changes in gene expression after short-term exposure of Arabidopsis thaliana cell cultures. Protoplasma 229, 155–162 (2006). https://doi.org/10.1007/s00709-006-0203-1

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