Skip to main content
SpringerLink
Log in

Morphogenetic stability of variegated Vanilla planifolia Jacks. plants micropropagated in a temporary immersion system (TIB®)

  • Published:
Rendiconti Lincei. Scienze Fisiche e Naturali Aims and scope Submit manuscript

Abstract

Key message

ISSR markers are useful to identify molecular changes in variegated vanilla (Vanilla planifolia Jacks.) plants derived from a temporary immersion system.

Abstract

Clonal propagation through in vitro cultures produces individuals that are identical to the parent plant from which they were subcultured. However, there is evidence of phenotypic variations between propagated clones. The most common phenotypic variations are changes in leaf pigmentation, i.e., variegated individuals. Many such phenotypic variations are exploited by the ornamental industry. In a previous study by our group on indirect organogenesis in vanilla (Ramírez-Mosqueda and Iglesias-Andreu in In Vitro Cell Dev Biol Plant 52:154–160, 2015), variegated Vanilla planifolia Jacks. plants were generated in vitro. A temporary immersion system allows for the mass propagation of plant material in liquid culture. The present study aimed to identify morphological and molecular changes that occurred in variegated plants obtained during micropropagation in a temporary immersion system, the TIB®. To achieve this, the morphology of plants micropropagated in TIB® was assessed and analyzed at the molecular level using inter-simple sequence repeats. Molecular variation was analyzed over four subcultures, each lasting 45 days. Plants showed 100% uniformity (monomorphism) and morphological stability, which were confirmed by the heritability of leaf pigmentation (variegated individuals) in all micropropagated plants over all four subcultures. This study demonstrates that high genetic and morphological stability was achieved in variegated V. planifolia genotypes that were micropropagated in TIB®. This would allow for the establishment of a clonal line of variegated plants with ornamental applications. In addition, 100% survival of the plants was observed during acclimatization. The study forms part of a wider long-term effort to assess the potential of mass propagation of variegated vanilla for ornamental purposes.

Graphic abstract

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

Abbreviations

BA:

6-Benzyladenine

TIB® :

Temporary immersion bioreactor

ISSR:

Inter-simple sequence repeats

MS:

Murashige and Skoog (1962) basal medium

PGR:

Plant growth regulator

References

  • Abdellatif KF, Hegazy AE, Aboshama HM, Emara HA, El-Shahed AA (2012) Morphological and molecular characterization of somaclonal variations in tissue culture-derived banana plants. J Genet Eng Biotechnol 10:47–53

    Article  Google Scholar 

  • Cardoso JC, Gerald LTC, Teixeira da Silva JA (2018) Micropropagation in the twenty-first century. In: Loyola-Vargas VM, Ochoa-Alejo N (eds) Plant cell culture protocols, methods in molecular biology, chapter 2, vol 1815. Springer Science + Business Media LLC, New York, pp 17–46

    Chapter  Google Scholar 

  • Chen J, Henny RJ (2006) Somaclonal variation: an important source for cultivar development of floriculture crops. In: Teixeira da Silva JA (ed) Floriculture, ornamental and plant biotechnology: advances and topical issues, vol II. Global Science Books, Isleworth, pp 244–253

    Google Scholar 

  • Chen M, Choi YD, Voytas DF, Rodermel S (2000) Mutations in the Arabidopsis VAR2 locus cause leaf variegation due to the loss of a chloroplast FtsH protease. Plant J 22:303–313

    Article  Google Scholar 

  • Duarte-Aké F, Castillo-Castro E, Pool FB, Espadas F, Santamaría JM, Ml Robert, De-la-Peña C (2016) Physiological differences and changes in global DNA methylation levels in Agave angustifolia Haw. albino variant somaclones during the micropropagation process. Plant Cell Rep 35:2489–2502

    Article  CAS  Google Scholar 

  • Escalona M, Lorenzo JC, Borroto CG, Daquinta MA (2003) Procedimiento y equipo para la propagación de plantas por inmersión temporal. Patente Cubana. No. 22947

  • Frómeta OM, Escalona Morgado MM, Teixeira da Silva JA, Pina Morgado DT, Daquinta Gradaille MA (2017) In vitro propagation of Gerbera jamesonii Bolus ex Hooker f. in a temporary immersion bioreactor. Plant Cell Tissue Organ Cult 129:543–551

    Article  CAS  Google Scholar 

  • Gantait S, Kundu S (2017) In vitro biotechnological approaches on Vanilla planifolia Andrews: advancements and opportunities. Acta Physiol Plant 39:196

    Article  CAS  Google Scholar 

  • Hassanein AM, Salem JM, Faheed FA, El-Nagish A (2018) Effect of anti-ethylene compounds on isoenzyme patterns and genome stability during long term culture of Moringa oleifera. Plant Cell Tissue Organ Cult 132:201–212

    Article  CAS  Google Scholar 

  • Hoffman PG, Zapf CM (2012) Review of vanilla. Medicinal and aromatic plants–industrial profiles. J Nat Prod 75:2272–2273

    Article  CAS  Google Scholar 

  • Hung CY, Xie JH (2009) A comparison of plants regenerated from a variegated Epipremnum aureum. Biol Plant 53:610–616

    Article  CAS  Google Scholar 

  • Hussain SA, Ahmad N, Anis M (2018) Synergetic effect of TDZ and BA on minimizing the post-exposure effects on axillary shoot proliferation and assessment of genetic fidelity in Rauvolfia tetraphylla (L.). Rend Fis Acc Lincei 29:109–115

    Article  Google Scholar 

  • Kato Y, Miura E, Matsushima R, Sakamoto W (2007) White leaf sectors in yellow variegated2 are formed by viable cells with undifferentiated plastids. Plant Physiol 144:952–960

    Article  CAS  Google Scholar 

  • Larkin PJ, Scowcroft WR (1981) Somaclonal variation—a novel source of variability from cell culture for plant improvement. Theor Appl Genet 60:197–214

    Article  CAS  Google Scholar 

  • Leva A, Rinaldi LMR (2017) Somaclonal variation. In: Thomas B, Murray BG, Murphy DJ (eds) Encyclopedia of applied plant sciences, 2nd edn. Academic Press, New York, pp 468–473

    Chapter  Google Scholar 

  • Martínez-Estrada E, Caamal-Velázquez JH, Salinas-Ruíz J, Bello-Bello JJ (2017) Assessment of somaclonal variation during sugarcane micropropagation in temporary immersion bioreactors by intersimple sequence repeat (ISSR) markers. In Vitro Cell Dev Biol Plant 53:553–560

    Article  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Naik D, Vartak V, Bhargava S (2003) Provenance- and subculture-dependent variation during micropropagation of Gmelina arbórea. Plant Cell Tissue Organ Cult 73:189–195

    Article  CAS  Google Scholar 

  • Pastelín-Solano MC, Salinas-Ruíz J, González-Arnao MT, Castañeda-Castro O, Galindo-Tovar ME, Bello-Bello JJ (2019) Evaluation of in vitro shoot multiplication and ISSR marker based assessment of somaclonal variants at different subcultures of vanilla (Vanilla planifolia Jacks). Physiol Mol Biol Plants 25:561–567

    Article  CAS  Google Scholar 

  • Pradeep R, Sarla N, Siddiq EA (2002) Inter simple sequence repeats (ISSR) polymorphism and its application in plant breeding. Euphytica 128:9–17

    Article  Google Scholar 

  • Ramírez-Mosqueda MA, Iglesias-Andreu LG (2015) Indirect organogenesis and assessment of somaclonal variation in plantlets of Vanilla planifolia Jacks. Plant Cell Tissue Organ Cult 123:657–664

    Article  Google Scholar 

  • Ramírez-Mosqueda MA, Iglesias-Andreu LG (2016) Evaluation of different temporary immersion systems (BIT®, BIG and RITA®) in the micropropagation of Vanilla planifolia Jacks. In Vitro Cell Dev Biol Plant 52:154–160

    Article  Google Scholar 

  • Ramírez-Mosqueda MA, Iglesias-Andreu LG, Ramírez-Madero Hernández-Rincón EU (2016) Micropropagation of Stevia rebaudiana Bert. in temporary immersion systems and evaluation of genetic fidelity. S Afr J Bot 106:238–243

    Article  CAS  Google Scholar 

  • Ramos-Castellá A, Iglesias-Andreu LG, Bello-Bello J, Lee-Espinosa H (2014) Improved propagation of vanilla (Vanilla planifolia Jacks. Ex Andrews) using a temporary immersion system. In Vitro Cell Dev Biol Plant 50:576–581

    Article  CAS  Google Scholar 

  • Roels S, Escalona M, Cejas I, Noceda C, Rodriguez R, Canal MJ, Sandoval J, Debergh P (2005) Optimization of plantain (Musa AAB) micropropagation by temporary immersion system. Plant Cell Tissue Organ Cult 82:57–66

    Article  CAS  Google Scholar 

  • Saha S, Dey T, Adhikari S, Mukhopadhyay S, Sengupta C, Ghosh P (2016) Effects of plant growth regulators on efficient plant regeneration efficiency and genetic stability analysis from two Ocimum tenuiflorum L. morphotypes. Rend Fis Acc Lincei 27:609–628

    Article  Google Scholar 

  • Sreedhar RV, Venkatachalam L, Bhagyalakshmi N (2007) Genetic fidelity of long-term micropropagated shoot cultures of vanilla (Vanilla planifolia Andrews) as assessed by molecular markers. Biotechnol J Healthc Nutr Technol 2:1007–1013

    CAS  Google Scholar 

  • Stewart CN Jr, Via LE (1993) A rapid CTAB DNA isolation technique useful for RAPD fingerprinting and other PCR applications. Biotechniques 14:748–751

    CAS  Google Scholar 

  • Us-Camas R, Castillo-Castro E, Aguilar-Espinosa M, Limones-Briones V, Rivera-Madrid R, Robert-Díaz ML, de-la-Peña C (2017) Assessment of molecular and epigenetic changes in the albinism of Agave angustifolia Haw. Plant Sci 263:156–167

    Article  CAS  Google Scholar 

  • Vidović M, Morinaa F, Prokić L, Milić-Komića S, Živanovića B, Veljović-Jovanović S (2016) Antioxidative response in variegated Pelargonium zonale leaves and generation of extracellular H2O2 in (peri)vascular tissue induced by sunlight and paraquat. J Plant Physiol 206:25–39

    Article  CAS  Google Scholar 

  • Zar JA (1996) Biostatistical analysis, 3rd edn. Prentice Hall Inc., New Jersey

Download references

Acknowledgements

The authors wish to thank the “Programa para el Desarrollo Profesional Docente” (Program for the Professional Development of Professors, PRODEP) (CA-UVER-234) for the financial support provided for the project “Biotechnological Basis for the Genetic Improvement of Vanilla planifolia” within the “Conservation, Management and Plant Breeding Network”.

Author information

Authors and Affiliations

  1. Colegio de Postgraduados Campus Córdoba, Km. 348 de La Carretera Federal Córdoba-Veracruz, Congregación Manuel León, Amatlán de los Reyes, Veracruz, Mexico

    Marco A. Ramírez-Mosqueda

  2. Facultad de Ciencias Biológicas y Agropecuarias, Universidad Veracruzana, C.P. 94945, Amatlan de los Reyes, Veracruz, Mexico

    Marco A. Ramírez-Mosqueda, Otto R. Leyva-Ovalle & Joaquín Murguía-González

  3. Instituto de Biotecnología y Ecología Aplicada (INBIOTECA), Universidad Veracruzana, Av. de las Culturas Veracruzanas No. 101, Campus para la Cultura, las Artes y el Deporte, Col. Emiliano Zapata, C.P. 91090, Xalapa, Veracruz, Mexico

    Lourdes G. Iglesias-Andreu & Enrique Favián-Vega

  4. P.O. Box 7, Miki-cho post office, Ikenobe 3011-2, Kagawa-ken, 761-0799, Japan

    Jaime A. Teixeira da Silva

Authors
  1. Marco A. Ramírez-Mosqueda
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Lourdes G. Iglesias-Andreu
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Enrique Favián-Vega
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jaime A. Teixeira da Silva
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Otto R. Leyva-Ovalle
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Joaquín Murguía-González
    View author publications

    You can also search for this author in PubMed Google Scholar

Contributions

LGIA, MARM and JATS devised and designed this research. MARM, ORLO, and JMG conducted the experiments. MARM and EFV performed and reviewed the molecular and statistical analyses. MARM, JATS and LGIA drafted the manuscript. LGIA, MARM, EFV, JATS, ORLO, and JMG reviewed and approved the manuscript.

Corresponding authors

Correspondence to Marco A. Ramírez-Mosqueda or Lourdes G. Iglesias-Andreu.

Ethics declarations

Conflict of interest

The authors declare that they have no conflicting interests.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Ramírez-Mosqueda, M.A., Iglesias-Andreu, L.G., Favián-Vega, E. et al. Morphogenetic stability of variegated Vanilla planifolia Jacks. plants micropropagated in a temporary immersion system (TIB®). Rend. Fis. Acc. Lincei 30, 603–609 (2019). https://doi.org/10.1007/s12210-019-00813-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12210-019-00813-9

Keywords

Search

Navigation