Abstract
The positive and reliable effect of temporary immersion systems on in vitroshoot proliferation was already proved for different plant genera and it is now presented as an alternative for plantain micropropagation. Some culture parameters affecting the efficiency of the twin flasks system or temporary immersion bioreactor (Escalona et al., 1999) were investigated. Three different cytokinins (benzyladenine, thidiazuron and meta-topolin) were added to the culture medium and meta-topolin at a concentration of 4.4 μM was proved to be the most efficient. Successive subcultures (28 days per subculture) were performed on medium supplemented with meta-topolin, revealing a decrease in multiplication after the 6th subculture. Multiplication rate was not changed within the ranges of immersion times (4, 12 or 22 min) and frequencies (every 3, 5 or 7 h) tested. The size of the bioreactor (250, 1,000, 5,000 or 10,000 ml) and the volume of medium per inoculum (10, 20 or 30 ml) were also evaluated and appeared to have an influence on the multiplication. A proportion of 25–100 ml of headspace per inoculum and 30 ml of medium per inoculum resulted in a multiplication rate > 13 in 28 days.
Similar content being viewed by others
Abbreviations
- BA:
-
N6-benzyladenine
- MET:
-
meta-topolin
- SP medium:
-
standard proliferation medium
- TDZ:
-
thidiazuron
- TIB:
-
temporary immersion bioreactor
References
D Alvard F Cote C Teisson (1993) ArticleTitleComparison of methods of liquid medium culture for banana micropropagation Plant Cell Tiss. Org. Cult. 32 55–60
G Arinaitwe PR Rubaihayo MJS Magambo (2000) ArticleTitleProliferation rate effects of cytokinins on banana (Musa spp.) cultivars Sci. Hortic. 86 13–21
Bhagyalakshmi NS Singh (1995) ArticleTitleRole of liquid versus agar-gelled media in mass propagation and ex vitro survival in bananas Plant Cell Tiss. Org. Cult. 41 71–73
MP De Proft LJ Maene PC Debergh (1985) ArticleTitleCarbon dioxide and ethylene evolution in the culture atmosphere of Magnolia cultured in vitro Physiol. Plant 65 375–379
M Escalona JC Lorenzo B González M Daquinta J González Y Desjardins CG Borroto (1999) ArticleTitlePineapple (Ananas comosus LMerr) micropropagation in temporary immersion systems. Plant Cell Rep. 18 743–748
H Etienne M Berthouly (2002) ArticleTitleTemporary immersion in plant micropropagation Plant Cell Tiss. Org. Cult. 69 215–231
FAO 2002: http://faostat.fao.org/faostat/collections?subset= agriculture
K Hirimburegama N Gamage (1997) ArticleTitleCultivar specificity with respect to in vitro micropropagation of Musa spp. (banana and plantain) J. Hortic Sci. 72 205–211
Y Israeli E Lahav O Reuveni (1995) In vitro culture of bananas S Gowen (Eds) Bananas and Plantains. Chapman and Hall London 47–178
RL Jarret W Rodriguez R Fernandez (1985) ArticleTitleEvaluation, tissue culture propagation, and dissemination of ‘Saba’ and ‘Pelipita’ plantains in Costa Rica Sci. Hortic 25 137–147
JC Lorenzo BL González M Escalona C Teisson P Espinosa C Borroto (1998) ArticleTitleSugarcane shoot formation in an improved temporary immersion system Plant Cell Tiss. Org. Cult. 54 197–200
BMJ Mendes SB Filippi CGB Demétrio APM Rodriguez (1999) ArticleTitleA statistical approach to study the dynamics of micropropagation rates, using banana (Musa spp.) as an example Plant Cell Rep. 18 967–971
T Murashige F Skoog (1962) ArticleTitleA revised medium for rapid growth and bioassays with tobacco tissue cultures Physiol. Plant 15 473–497
SJ Murch C Liu RM Romero PK Saxena (2004) ArticleTitleIn vitro culture and temporary immersion bioreactor production of␣Crescentia cujete. Plant Cell Tiss. Org. Cult. 78 63–68
R Ortiz DR Vuylsteke (1994) ArticleTitleGenetics of apical dominance in plantain (Musa spp AAB group) and improvement of suckering behavior. J. Am. Soc. Hortic Sci. 119 1050–1053
SPO Werbrouck M Strnad HA VanOnckelen PC Debergh (1996) ArticleTitleMeta-topolin, an alternative to benzyladenine in tissue culture? Physiol Plant 98 291–297
A Wojtania E Gabryszweska (2001) ArticleTitleEffect of cytokinins and amino acids on multiplication of Pelargonium cultivars Acta Soc. Bot. Pol. 70 203–207
WC Wong (1986) ArticleTitleIn vitro propagation of banana (Musaspp.): initiation, proliferation and development of shoot-tip cultures on defined media Plant Cell Tiss. Org. Cult. 6 159–166
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Roels, S., Escalona, M., Cejas, I. et al. Optimization of plantain (Musa AAB) micropropagation by temporary immersion system. Plant Cell Tiss Organ Cult 82, 57–66 (2005). https://doi.org/10.1007/s11240-004-6746-y
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11240-004-6746-y