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Encapsulation technology for short-term storage and germplasm exchange of Vitex trifolia L.

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Abstract

The current study reports the encapsulation of nodal segment of V. trifolia L. excised from 2-month-old in vitro-raised cultures for short-term conservation and propagation. The encapsulation of nodal segments was significantly affected by the concentrations of sodium alginate (Na alginate) and calcium chloride (CaCl2 2H2O). The best gel complex using 3 % sodium alginate and 100 mM CaCl2 2H2O was found most suitable for the production of ideal Ca alginate beads. Maximum percent conversion response (84.9 %) was recorded on Murashige and Skoog (MS) basal medium supplemented with 5.0 µM 6-benzyladenine (BA), 0.5 µM α-naphthalene acetic acid (NAA) after 6 weeks of culture. The encapsulated nodal segments could be stored at 4 °C up to 8 weeks with 42.5 % regeneration efficiency. Plantlets obtained were rooted best on full-strength MS medium containing 0.5 µM α-naphthalene acetic acid (NAA) for the production of complete plantlets. The regenerated plantlets were successfully hardened and established in field where they grew well without any detectable variation with a survival rate of 92 %. The high frequency of plant re-growth from alginate-coated nodal segments coupled with high viability percentage after 4 weeks of storage is highly encouraging for the exchange of V. trifolia genetic resources.

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Abbreviations

CaCl22H2O:

Calcium chloride

BA:

6-Benzyladenine

IBA:

Indole-3-butyric acid

NAA:

α-Naphthalene acetic acid

MS:

Murashige and Skoog (1962) medium

PGR:

Plant growth regulator

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Acknowledgments

Md Rafique Ahmed thanks the University Grants Commission (UGC), Government of India, New Delhi for the award of Senior Research Fellowship under MANF. Research support provided by the Department of Science and Technology (DST) in the form of DST-FIST (2011–16) and UGC in the form of UGC-SAP; DRS-I Programme (2009–2014) is duly acknowledged.

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Authors and Affiliations

  1. Department of Botany, Plant Biotechnology Laboratory, Aligarh Muslim University, Aligarh, 202 002, India

    Md Rafique Ahmed & Mohammad Anis

  2. Department of Plant Production, College of Food and Agricultural Sciences, King Saud University, PO Box 2460, Riyadh, 11451, Saudi Arabia

    Mohammad Anis & Hashim A. Al-Etta

Authors
  1. Md Rafique Ahmed
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  2. Mohammad Anis
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  3. Hashim A. Al-Etta
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Correspondence to Mohammad Anis.

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Ahmed, M.R., Anis, M. & Al-Etta, H.A. Encapsulation technology for short-term storage and germplasm exchange of Vitex trifolia L.. Rend. Fis. Acc. Lincei 26, 133–139 (2015). https://doi.org/10.1007/s12210-014-0366-1

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