Abstract
The monoclonal antibody (mAb) protein class has become a primary therapeutic platform for the production of new life saving drug products. MAbs are comprised of two domains: the antigen-binding fragment (Fab) and crystallizable fragment (Fc). Despite the success in the clinic, NMR assignments of the complete Fab domain have been elusive, in part due to problems in production of properly folded, triply-labeled 2H,13C,15N Fab domain. Here, we report the successful recombinant expression of a triply-labeled Fab domain, derived from the standard IgG1κ known as NISTmAb, in yeast. Using the 2H,13C,15N Fab domain, we assigned 94% of the 1H, 13C, and 15N backbone atoms.
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Data Availability
The assigned 1H, 13C, 15N chemical shifts have been deposited in the BMRB (https://www.bmrb.wisc.edu/) under accession number 51696.
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Acknowledgements
The authors acknowledge the support by NIST Biomanufacturing Program and NIST for support of biomolecular NMR instrumentation.
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YA,WO,JM&RB: Conceptualization; TS,KC,GG&RB: Investigation; TS&RB: Formal Analysis; TS:Validation; TS: Visualization; TS&RB: Writing-original draft; TS,KC,YA,WO,JM&RB: Writing-review and editing; RB:Supervision.
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Solomon, T.L., Chao, K., Gingras, G. et al. Backbone NMR assignment of the yeast expressed Fab fragment of the NISTmAb reference antibody. Biomol NMR Assign 17, 75–81 (2023). https://doi.org/10.1007/s12104-023-10123-9
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DOI: https://doi.org/10.1007/s12104-023-10123-9