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Nano Zinc Versus Bulk Zinc Form as Dietary Supplied: Effects on Growth, Intestinal Enzymes and Topography, and Hemato-biochemical and Oxidative Stress Biomarker in Nile Tilapia (Oreochromis niloticus Linnaeus, 1758)

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Abstract

Five isonitrogenous diets were formulated to comprise two forms of zinc (Zn): convention zinc oxide named Bulk-ZnO or zinc oxide nanoparticles (Nano-ZnO) supplemented at two levels 30 and 60 mg kg−1 compared to the control diet. Nile tilapia, Oreochromis niloticus, fingerlings (5.02–5.05 g) were fed tested diets two times a day for 84 days. The results displayed that the best growth and digestive enzyme activity (P < 0.05) were noticed in fish fed 60 mg kg−1 Nano-ZnO. Moreover, significant (P < 0.05) improvement in intestinal topography was observed in 60 mg kg−1 Nano-ZnO group versus other treatments. Furthermore, fish fed 30 mg kg−1 Nano-ZnO recorded the best values of hematological indices (P < 0.05). The alanine and aspartate aminotransferase (ALT and AST) values were lower, while total serum protein, albumin, and globulin contents were clearly higher in fish fed diet that contained 30 mg kg−1 Nano-ZnO versus other groups. The significant highest values of oxidative enzyme activity escorted with lower malondialdehyde value recorded of fish fed diet supplemented with 60 mg kg−1 Nano-ZnO. The results indicated that inclusion of Nano-ZnO at 60 mg kg−1 was the recommended source to enhance growth, feed utilization, amylase and lipase enzymes activity, intestinal morphology, hemato-biochemical, and oxidative response biomarkers of Nile tilapia compared with Bulk-ZnO in commercial tilapia feeds.

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Acknowledgements

The authors acknowledge the Animal Production Department, Faculty of Agriculture, Benha University, Cairo, Egypt, for supporting and financial assistance during this research project.

Funding

This study was funded by the Benha University, Egypt.

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  1. Department of Aquaculture, Central Laboratory of Aquaculture Research, Agriculture Research Center, Abbassa, Abo-Hammad, Sharqia, Egypt

    Mohamed S. Ibrahim & Ahmed I. Ahmed

  2. Department of Animal Production, Fish Research Laboratory, Faculty of Agriculture at Moshtohor, Benha University, Benha, 13736, Egypt

    Gaffar M. I. El-Gendi & Mohamed S. Hassaan

  3. Animal Production Department, Faculty of Agriculture, Cairo University, Cairo, Egypt

    Ehab R. El-Haroun

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  1. Mohamed S. Ibrahim
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The authors declared that the current manuscript prepared from the Ph. D student project to submit his thesis paper work to the GS to get approve to setup his defense date and time and nominate the external examiner after received the acceptance of the manuscript.

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Ibrahim, M.S., El-Gendi, G.M.I., Ahmed, A.I. et al. Nano Zinc Versus Bulk Zinc Form as Dietary Supplied: Effects on Growth, Intestinal Enzymes and Topography, and Hemato-biochemical and Oxidative Stress Biomarker in Nile Tilapia (Oreochromis niloticus Linnaeus, 1758). Biol Trace Elem Res 200, 1347–1360 (2022). https://doi.org/10.1007/s12011-021-02724-z

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