Skip to main content
SpringerLink
Log in

One-Step Bulk Preparation of Calcium Carbonate Nanotubes and Its Application in Anticancer Drug Delivery

  • Published:
Biological Trace Element Research Aims and scope Submit manuscript

Abstract

Bulk fabrication of ordered hollow structural particles (HSPs) with large surface area and high biocompatibility simultaneously is critical for the practical application of HSPs in biosensing and drug delivery. In this article, we describe a smart approach for batch synthesis of calcium carbonate nanotubes (CCNTs) based on supported liquid membrane (SLM) with large surface area, excellent structural stability, prominent biocompatibility, and acid degradability. The products were characterized by transmission electron micrograph, X-ray diffraction, Fourier transform infrared spectra, UV–vis spectroscopy, zeta potential, and particle size distribution. The results showed that the tube-like structure facilitated podophyllotoxin (PPT) diffusion into the cavity of hollow structure, and the drug loading and encapsulation efficiency of CCNTs for PPT are as high as 38.5 and 64.4 wt.%, respectively. In vitro drug release study showed that PPT was released from the CCNTs in a pH-controlled and time-dependent manner. The treatment of HEK 293T and SGC 7901 cells demonstrated that PPT-loaded CCNTs were less toxic to normal cells and more effective in antitumor potency compared with free drugs. In addition, PPT-loaded CCNTs also enhanced the apoptotic process on tumor cells compared with the free drugs. This study not only provides a new kind of biocompatible and pH-sensitive nanomaterial as the feasible drug container and carrier but more importantly establishes a facile approach to synthesize novel hollow structural particles on a large scale based on SLM technology.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Scheme 1
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

References

  1. Luo B, Xu S, Luo A et al (2011) Mesoporous biocompatible and acid-degradable magnetic colloidal nanocrystal clusters with sustainable stability and high hydrophobic drug loading capacity. ACS Nano 5:1428–1435. doi:10.1021/nn103213y

    Article  PubMed  CAS  Google Scholar 

  2. Khajeh M (2010) Silver nanoparticles for the adsorption of manganese from biological samples. Biol Trace Elem Res 138:337–345. doi:10.1007/s12011-009-8600-x

    Article  PubMed  CAS  Google Scholar 

  3. Wang WR, Zhu RR, Xiao R et al (2011) The electrostatic interactions between nano-TiO2 and trypsin inhibit the enzyme activity and change the secondary structure of trypsin. Biol Trace Elem Res 142:435–446. doi:10.1007/s12011-010-8823-x

    Article  PubMed  CAS  Google Scholar 

  4. Zhang YZ, Li HR, Dai J et al (2010) Spectroscopic studies on the binding of cobalt (II) 1,10-phenanthroline complex to bovine serum albumin. Biol Trace Elem Res 135:136–152. doi:10.1007/s12011-009-8502-y

    Article  PubMed  CAS  Google Scholar 

  5. Au C, Mutkus L, Dobson A et al (2007) Effects of nanoparticles on the adhesion and cell viability on astrocytes. Biol Trace Elem Res 120:248–256. doi:10.1007/s12011-007-0067-z

    Article  PubMed  CAS  Google Scholar 

  6. Kong B, Zhu AW, Luo YP et al (2011) Sensitive and selective colorimetric visualization of cerebral dopamine based on double molecular recognition. Angew Chem Int Ed 50:1837–1840. doi:10.1002/anie.201007071

    Article  CAS  Google Scholar 

  7. Allen TM, Cullis PR (2004) Drug delivery systems: entering the mainstream. Science 303:1818–1822. doi:10.1126/science.1095833

    Article  PubMed  CAS  Google Scholar 

  8. Arnold AM (1979) Podophyllotoxin derivative VP 16-213. Cancer Chemother Pharmacol 3:71–80. doi:10.1007/BF00254976

    Article  PubMed  CAS  Google Scholar 

  9. Tian X, Zhang FM, Lib WG (2002) Antitumor and antioxidant activity of spin labeled derivatives of podophyllotoxin (GP-1) and congeners. Life Sci 70:2433–2443

    Article  PubMed  CAS  Google Scholar 

  10. Gordaliza M, Castro MA, DelCorral JM et al (2000) Antitumor properties of podophyllotoxin and related compounds. Curr Pharm Des 6:1811–1839. doi:10.2174/1381612003398582

    Article  PubMed  CAS  Google Scholar 

  11. Van-maanen JMS, Retal J, De-vries J et al (1988) Mechanism of action of antitumor drug etoposide: a review. J Natl Cancer Inst 80:1526–1533. doi:10.1093/jnci/80.19.1526

    Article  PubMed  CAS  Google Scholar 

  12. Shah JC, Chen JR, Chow D (1989) Preformulation study of etoposide: identification of physicochemical characteristics responsible for the low and erratic oral bioavailability of etoposide. Pharm Res 6:408–412. doi:10.1023/A:1015935532725

    Article  PubMed  CAS  Google Scholar 

  13. Li A, Qin LL, Zhu D et al (2010) Signalling pathways involved in the activation of dendritic cells by layered double hydroxide nanoparticles. Biomaterials 31:748–756. doi:10.1016/j.biomaterials.2009.09.095

    Article  PubMed  Google Scholar 

  14. Li A, Qin LL, Wang WR et al (2011) The use of layered double hydroxides as DNA vaccine delivery vector for enhancement of anti-melanoma immune response. Biomaterials 2:469–477. doi:10.1016/j.biomaterials.2010.08.107

    Article  Google Scholar 

  15. Xiao R, Wang WR, Pan LL et al (2011) A sustained folic acid release system based on ternary magnesium/zinc/aluminum layered double hydroxides. J Mater Sci 46:2635–2643. doi:10.1007/s10853-010-5118-8

    Article  CAS  Google Scholar 

  16. Qin LL, Xue M, Wang WR et al (2010) The in vitro and in vivo anti-tumor effect of layered double hydroxides nanoparticles as delivery for podophyllotoxin. Int J Pharm 388:223–230. doi:10.1016/j.ijpharm.2009.12.044

    Article  PubMed  CAS  Google Scholar 

  17. Zhu RR, Qin LL, Wang M et al (2009) Preparation, characterization and anti-tumor property of podophyllotoxin-loaded solid lipid nanoparticles. Nanotechnology 20:055702 (7 pp). doi:10.1088/0957-4484/20/5/055702

    Google Scholar 

  18. Qin LL, Wang SL, Zhang R et al (2008) Two different approaches to synthesizing Mg–Al-layered double hydroxides as folic acid carriers. J Phys Chem Solids 69:2779–2784. doi:10.1016/j.jpcs.2008.06.144

    Article  CAS  Google Scholar 

  19. Lee Y, Lee J, Bae CJ, Park JG, Noh HJ, Park JH, Hyeon T (2005) Large-scale synthesis of uniform and crystalline magnetite nanoparticles using reverse micelles as nanoreactors under reflux conditions. Adv Funct Mater 15:503–509. doi:10.1002/adfm.200400187

    Article  CAS  Google Scholar 

  20. Dolinska B, Mikulska A, Caban A et al (1998) A model for calcium permeation into small intestine. Biol Trace Elem Res 142:456–464. doi:10.1007/s12011-010-8827-6

    Article  Google Scholar 

  21. Helmlinger G, Yuan F, Dellian M, Jain RK (1997) Interstitial pH and pO2 gradients in solid tumors in vivo: high-resolution measurements reveal a lack of correlation. Nat Med 3:177–182. doi:10.1038/nm0297-177

    Article  PubMed  CAS  Google Scholar 

  22. Wei W, Ma GH, Hu G et al (2008) Preparation of hierarchical hollow CaCO3 particles and the application as anticancer drug carrier. J Am Chem Soc 130:15808–15810. doi:10.1021/ja8039585

    Article  PubMed  CAS  Google Scholar 

  23. Lee Y, Lee J, Bae CJ et al (2005) Large-scale synthesis of uniform and crystalline magnetite nanoparticles using reverse micelles as nanoreactors under reflux conditions. Ad Funct Mater 15:503–509. doi:10.1002/adfm.200400187

    Article  CAS  Google Scholar 

  24. Han S, Jang B, Oh SM et al (2005) Simple synthesis of hollow tin dioxide microspheres and their application to lithium-ion battery anodes. Ad Funct Mater 15:1845–1850. doi:10.1002/adfm.200500243

    Article  CAS  Google Scholar 

  25. Caruso F, Caruso RA, Mohwald H (1998) Nanoengineering of inorganic and hybrid hollow spheres by colloidal templating. Science 282:1111–1114. doi:10.1126/science.282.5391.1111

    Article  PubMed  CAS  Google Scholar 

  26. Loges N, Graf K, Nasdala L et al (2006) Probing cooperative interactions of tailor-made nucleation surfaces and macromolecules: a bioinspired route to hollow micrometer-sized calcium carbonate particles. Langmuir 22:3073–3080. doi:10.1021/la0528596

    Article  PubMed  CAS  Google Scholar 

  27. Suzuki M, Nagasawa H, Kogure T (2006) Synthesis and structure of hollow calcite particles. Cryst Growth Des 6:2004–2006. doi:10.1021/cg0602921

    Article  CAS  Google Scholar 

  28. Sugihara H, Inoue K, Nakayama M et al (2009) A novel nanotube of composite of calcium carbonate and calcium sulfate. Mater Lett 63:322–324. doi:10.1016/j.matlet.2008.10.025

    Article  CAS  Google Scholar 

  29. Yu H, Yu J, Liu S et al (2007) Template-free hydrothermal synthesis of CuO/Cu2O composite hollow microspheres. Chem Mater 19:4327–4334. doi:10.1021/cm070386d

    Article  CAS  Google Scholar 

  30. Sun DM, Zhu DZ (2009) Bi-template effect of a vegetal system on the synthesis of alkaline-earth tungstate nanocrystals. J Mater Res 24:347–351. doi:10.1557/JMR.2009.0072

    Article  CAS  Google Scholar 

  31. Sun DM, Wu QS (2006) A novel method for crystal control: synthesis and design of strontium carbonate with different morphologies by supported liquid membrane. J Appl Crystallogr 39:544–549. doi:10.1107/S0021889806015925

    Article  CAS  Google Scholar 

  32. Wu QS, Sun DM, Liu HJ et al (2004) Abnormal polymorph conversion of calcium carbonate and nano-self-assembly of vaterite by a supported liquid membrane system. Cryst Growth Des 4:717–720. doi:10.1021/cg034247u

    Article  CAS  Google Scholar 

  33. Sun DM, Zhu DZ (2008) Synthesis and design of MnCO3 crystals with different morphologies by supported liquid membrane. J Chem Crystallogr 38:949–952. doi:10.1007/s10870-008-9419-6

    Article  CAS  Google Scholar 

  34. Mosmann T (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. J Immunol Method 65:55–63. doi:10.1016/0022-1759(83)90303-4

    Article  CAS  Google Scholar 

  35. Addadi L, Weiner S (1992) Control and design principles in biological mineralization. Angew Chem Int Ed 31:153–169. doi:10.1002/anie.199201531

    Article  Google Scholar 

  36. Naka K, Keum DK, Tanaka Y et al (2000) Control of crystal polymorphs by a ‘latent inductor’: crystallization of calcium carbonate in conjunction with in situ radical polymerization of sodium acrylate in aqueous solution. J Chem Soc 16:1537–1538. doi:10.1039/b004649n

    Google Scholar 

  37. Mann S (2001) Biomineralization. Principles and concepts in bioinorganic materials chemistry. Oxford University Press, New York, p 104

    Google Scholar 

  38. Smith BL, Schaffer TE, Viani M et al (1999) Molecular mechanistic origin of the toughness of natural adhesives, fibres and composites. Nature 399:761–763. doi:10.1038/21607

    Article  CAS  Google Scholar 

  39. Sangwal K (1996) Growth kinetics and surface morphology of crystals grown from solutions: recent observations and their interpretations. Prog Cryst Growth Charact 36:163–248. doi:10.1016/S0960-8974(98)00009-6

    Google Scholar 

  40. Mann S, Didymus JM, Sanderson NP et al (1990) Morphological influence of functionalized and non-functionalized α, ω-dicarboxylates on calcite crystallization. J Chem Soc Faraday Trans 86:1873–1880. doi:10.1039/FT9908601873

    Article  CAS  Google Scholar 

  41. Didymus JM, Oliver P, Mann S (1993) Influence of low-molecular-weight and macromolecular organic additives on the morphology of calcium carbonate. J Chem Soc Faraday Trans 89:2891–2900. doi:10.1039/FT9938902891

    Article  CAS  Google Scholar 

  42. Shivkumara C, Singh P, Gupta A et al (2006) Synthesis of vaterite CaCO3 by direct precipitation using glycine and L-alanine as directing agents. Mater Res Bull 41:1455–1460. doi:10.1016/j.materresbull.2006.01.026

    Article  CAS  Google Scholar 

  43. Nan ZD, Chen XN, Yang QQ et al (2008) Structure transition from aragonite to vaterite and calcite by the assistance of SDBS. J Colloid Interface Sci 325:331–336. doi:10.1016/j.jcis.2008.05.045

    Article  PubMed  CAS  Google Scholar 

  44. Lee MK, Lim SJ, Kim CK (2007) Preparation, characterization and in vitro cytotoxicity of paclitaxel-loaded sterically stabilized solid lipid nanoparticles. Biomaterials 28:2137–2146. doi:10.1016/j.biomaterials.2007.01.014

    Article  PubMed  CAS  Google Scholar 

  45. Roberts JE, Wielgus AR, Boyes WK et al (2008) Phototoxicity and cytotoxicity of fullerol in human lens epithelial cell. Toxicol Appl Pharmacol 228:49–58. doi:10.1016/j.taap.2009.09.021

    Article  PubMed  CAS  Google Scholar 

  46. Choy JH, Jung JS, Oh JM (2004) Layered double hydroxide as an efficient drug reservoir for folate derivatives. Biomaterials 25:3059–3064. doi:10.1016/j.biomaterials.2003.09.083

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was financially supported by the Major State Basic Research Development Program of China (973 Program, grant no. 2010CB933901), the National Natural Science Foundation of China (grant no. 50802063, 31140038), the Research Fund for the Doctoral Program of Higher Education of China (grant no. 20090072120019), Science and Technology Commission of Shanghai Municipality (grant no. 11411951500), the Fundamental Research Funds for the Central Universities, and Young Excellent Talents Plans in Tongji University.

Author information

Authors and Affiliations

  1. School of Life Science and Technology, Tongji University, 1239 Siping Road, Shanghai, 200092, People’s Republic of China

    Jing Tang, Dong-Mei Sun, Wen-Yu Qian, Rong-Rong Zhu, Xiao-Yu Sun, Wen-Rui Wang, Kun Li & Shi-Long Wang

Authors
  1. Jing Tang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Dong-Mei Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Wen-Yu Qian
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rong-Rong Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Xiao-Yu Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Wen-Rui Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Kun Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Shi-Long Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Shi-Long Wang.

Additional information

Jing Tang and Dong-Mei Sun contributed equally to this work.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tang, J., Sun, DM., Qian, WY. et al. One-Step Bulk Preparation of Calcium Carbonate Nanotubes and Its Application in Anticancer Drug Delivery. Biol Trace Elem Res 147, 408–417 (2012). https://doi.org/10.1007/s12011-012-9325-9

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12011-012-9325-9

Keywords

Search

Navigation