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Spectroscopic Investigation of the Interaction Between Copper (II) 2-oxo-propionic Acid Salicyloyl Hydrazone Complex and Bovine Serum Albumin

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Abstract

The interaction between copper (II) 2-oxo-propionic acid salicyloyl hydrazone (CuIIL) and bovine serum albumin (BSA) under physiological conditions was investigated by the methods of fluorescence spectroscopy, UV-Vis absorption, and circular dichroism spectroscopy. Fluorescence data showed that the fluorescence quenching of BSA by CuIIL was the result of the formation of the BSA–CuIIL complex. The apparent binding constants (K a) between CuIIL and BSA at four different temperatures were obtained according to the modified Stern–Volmer equation. The thermodynamic parameters, enthalpy change (ΔH) and entropy change (ΔS), for the reaction were calculated to be −80.79 kJ mol−1 and −175.48 J mol−1 K−1 according to van’t Hoff equation. The results indicated that van der Waals force and hydrogen bonds were the dominant intermolecular force in stabilizing the complex. The binding distance (r) between CuIIL and the tryptophan residue of BSA was obtained to be 4.1 nm according to Förster’s nonradioactive energy transfer theory. The conformational investigation showed that the application of CuIIL increased the hydrophobicity of amino acid residues and decreased the α-helical content of BSA (from 62.71% to 37.31%), which confirmed some microenvironmental and conformational changes of BSA molecules.

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Acknowledgments

We gratefully acknowledge the financial support of National Natural Science Foundation of China (Grant nos. 30570015, 20621502), Natural Science Foundation of Hubei Province (2005ABC002), and the Research Foundation of Chinese Ministry of Education (no. [2006]8-IRT0543).

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Authors and Affiliations

  1. Department of Chemistry, College of Chemistry and Environmental Engineering, Yangtze University, Jingzhou, Hubei, 434023, People’s Republic of China

    Ping Mei, Ye-Zhong Zhang, Xiao-Ping Zhang, Cheng-Xiang Yan & Yi Liu

  2. State Key Laboratory of Virology, College of Chemistry and Molecular Sciences, Wuhan University, Wuhan, Hubei, 430072, China

    Ye-Zhong Zhang, Hua Zhang & Yi Liu

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  1. Ping Mei
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  2. Ye-Zhong Zhang
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Correspondence to Yi Liu.

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Mei, P., Zhang, YZ., Zhang, XP. et al. Spectroscopic Investigation of the Interaction Between Copper (II) 2-oxo-propionic Acid Salicyloyl Hydrazone Complex and Bovine Serum Albumin. Biol Trace Elem Res 124, 269–282 (2008). https://doi.org/10.1007/s12011-008-8147-2

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