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Spectroscopic and electrochemical studies on the molecular interaction between copper sulphide nanoparticles and bovine serum albumin

  • Biomaterials
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Abstract

The interaction of covellite hexagonal phase of copper sulphide nanoparticles (CuS NPs) with bovine serum albumin (BSA) was examined systematically by using fluorescence, UV–visible, circular dichroism (CD), Fourier transform infrared (FTIR), dynamic light scattering (DLS) and molecular modelling techniques. Electrochemical method was studied to further confirm the interaction of BSA with CuS NPs. The results of fluorescence studies demonstrated that fluorescence of BSA was quenched by CuS NPs via a static quenching mechanism. The negative values of thermodynamic parameters (ΔG, ΔH and ΔS) indicated that the binding process is spontaneous, exothermic and van der Waals force or hydrogen bonding plays major roles in the interaction of CuS NPs with BSA. The interaction of CuS NPs with Trp residue was established by synchronous studies, and competitive binding studies revealed that Trp-212 of subdomain IIA was involved in the interaction with these nanoparticles. Further, the efficiency of energy transferred and the distance between fluorophore (BSA) and acceptor (CuS NPs) were calculated using Forster’s resonance energy transfer theory. The results of UV–visible, CD, FTIR and DLS revealed that the CuS NPs interact with BSA by inducing the conformational changes in secondary structure and reducing the α-helix content of BSA. Molecular modelling studies suggested that CuS NPs bind to site I of sub domain IIA of BSA. The results of spectroscopic and molecular docking studies were complimented by the electrochemical techniques.

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Acknowledgements

Shivaraj Yellappa acknowledge the Raman Fellowship received from University Grants Commission [F. No. 5-120/2016(IC)], India, Prof. Raghavan Varadarajan, Department of Molecular Biophysics Unit, Indian Institute of Science, Bengaluru, to provide a circular dichroism spectrophotometer instrument facility and Prof. Francis D’souza, Department of Chemistry, University of North Texas, USA for technical discussion. Thanks to Mr. Sandeep, Application specialist, Malvern-Aimil application centre for assisting the DLS studies.

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Authors and Affiliations

  1. Department of Chemistry, Government Science College, Bengaluru, 560001, India

    Mallappa Mahanthappa & Shivaraj Yellappa

  2. Research Resource Centre, Visvesvaraya Technological University, Belagum, 590018, India

    Mallappa Mahanthappa

  3. Department of Biochemistry, Karnatak University, Dharwad, 580003, India

    Mohammed Azharuddin Savanur

  4. Department of Cellular and Molecular Biology, University of Texas Health Science Centre, Tyler, TX, USA

    Bijesh Puthusseri

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  1. Mallappa Mahanthappa
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  2. Mohammed Azharuddin Savanur
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  3. Bijesh Puthusseri
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  4. Shivaraj Yellappa
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Correspondence to Shivaraj Yellappa.

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Mahanthappa, M., Savanur, M.A., Puthusseri, B. et al. Spectroscopic and electrochemical studies on the molecular interaction between copper sulphide nanoparticles and bovine serum albumin. J Mater Sci 53, 202–214 (2018). https://doi.org/10.1007/s10853-017-1521-8

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  • DOI: https://doi.org/10.1007/s10853-017-1521-8

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