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RAPD and ISSR Marker-Based Comparative Evaluation of Genetic Diversity Among Indian Germplasms of Euryale ferox: an Aquatic Food Plant

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Abstract

Euryale ferox Salisbury is an important aquatic food plant cultivated largely in eastern India. E. ferox is a monotypic genus, and breeding programmes have mostly relied on the variability present in the primary gene pool. Knowledge of the genetic structure of the population is limited, and there are very few reports available on the genetic diversity of E. ferox. In this study, comprehensive research on the genetic diversity of 16 germplasms of E. ferox was carried out using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers. Out of 320 RAPD and 95 ISSR primers screened initially, 61 primers (40 RAPD and 21 ISSR) gave reproducible bands and were selected for further work. Amplification of the 40 RAPD primers gave 533 polymorphic bands with an average of 13.32 polymorphic bands per primer. The percentage of polymorphism ranged from 37.5 to 100, with an average of 88.3 %. The 21 ISSR primers produced 259 bands, of which 214 were polymorphic, with an average of 10.19 polymorphic bands per primer. The percentage of polymorphism using ISSR primers ranged from 50 to 100, with a mean of 82.6 %. Jaccard’s coefficient ranged from 0.45 to 0.69 (RAPD), 0.50 to 0.77 (ISSR) and 0.48 to 0.71 (RAPD and ISSR). Molecular characterization of different germplasms of E. ferox not only is essential for its conservation but also can be used in further breeding programmes.

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Acknowledgments

The authors are thankful to different farmers of Bihar and Research Centre Makhana, Darbhanga, for providing the diverse germplasms of E. ferox. This work is supported by a financial grant (Grant No.: SB/YS/LS-136/2013) from DST-SERB, New Delhi, India.

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Authors and Affiliations

  1. Centre for Biological Sciences (Biotechnology), School of Earth, Biological and Environmental Sciences, Central University of South Bihar, Patna, Bihar, 800014, India

    Hemant Kumar, Priti Priya, Nena Singh, Mukesh Kumar & Nitish Kumar

  2. ICAR-National Institute of Biotic Stress Management, Baronda farm, Raipur, Chhattisgarh, 493225, India

    Binod Kumar Choudhary

  3. ICAR-Indian Institute of Wheat & Barley Research, Karnal, Haryana, 132001, India

    Lokendra Kumar

  4. ICAR RCER-Research Centre for Makhana, Darbhanga, Bihar, 8473016, India

    Indu Shekhar Singh

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  1. Hemant Kumar
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  2. Priti Priya
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  3. Nena Singh
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  4. Mukesh Kumar
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  5. Binod Kumar Choudhary
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  8. Nitish Kumar
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Correspondence to Nitish Kumar.

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Kumar, H., Priya, P., Singh, N. et al. RAPD and ISSR Marker-Based Comparative Evaluation of Genetic Diversity Among Indian Germplasms of Euryale ferox: an Aquatic Food Plant. Appl Biochem Biotechnol 180, 1345–1360 (2016). https://doi.org/10.1007/s12010-016-2171-z

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