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Genetic similarity among cultivars of Phyllostachys pubescens

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Abstract

Phyllostachys pubescens is the most important economic bamboo species in China, which grows widely in the South of China. There are more than ten cultivars in this species but their genetic relationship still remains unknown. We used both amplified fragment length polymorphism (AFLP) and inter-simple sequence repeat (ISSR) techniques to determine genetic similarity among ten cultivars of P. pubescens and two related species. Eight hundred and twenty seven bands, in which 495 are polymorphic, were detected using 15 pairs of AFLP primers whereas total 231 bands, in which 154 bands are polymorphic, were scored using 16 ISSR primers. Statistic analysis showed that the genetic similarity matrices obtained from these two sets of molecular markers had a significant correlation (R = 0.959, P = 0.013). The dendrogram generated with AFLP and ISSR markers could clearly genetically identify ten cultivars of P. pubescens that had high similarity with genetic distances ranging from 0.023 to 0.108, and could be divided into three groups based on their genetic variation and similarity. Our results suggest that these molecular markers are useful to genetically classify cultivars or varieties of a species, particularly a bamboo species.

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Abbreviations

AFLP:

Amplified fragment length polymorphism

ISSR:

Inter-simple sequence repeat

RFLP:

Restriction fragment length polymorphisms

RAPD:

Random amplified polymorphic DNA

CTAB:

Cetyl-trimethylammonium bromide

PCR:

Polymerase chain reaction

UPGMA:

Unweighted pair-group method with arithmetic means

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Acknowledgments

We thank Dr. Xuerong Shi for critical reading of the manuscript, and Mr. Chang Ping Zhou for providing us with bamboo leaf samples. This study was supported by research grants from the Science and Technology Department of Zhejiang Province, China [grants #2004C12034 and #2005C12002–01].

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Authors and Affiliations

  1. Key Lab for Modern Silvicultural Technology of Zhejiang Province, Zhejiang Forestry University, North Circular Road, 88, 311300, Lin’an, Zhejiang, People’s Republic of China

    Xin Chun Lin, Xiao Sai Ruan, Yong Feng Lou, Xiao Qin Guo & Wei Fang

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  1. Xin Chun Lin
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  2. Xiao Sai Ruan
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  3. Yong Feng Lou
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  4. Xiao Qin Guo
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  5. Wei Fang
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Correspondence to Wei Fang.

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Lin, X.C., Ruan, X.S., Lou, Y.F. et al. Genetic similarity among cultivars of Phyllostachys pubescens . Plant Syst Evol 277, 67–73 (2009). https://doi.org/10.1007/s00606-008-0104-1

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  • DOI: https://doi.org/10.1007/s00606-008-0104-1

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