Abstract
The platform chemical 2,3-butanediol (2,3-BDO) is a valuable product that can be converted into several petroleum-based chemicals via simple chemical reactions. Here, we produced 2,3-BDO with the non-pathogenic and rapidly growing Corynebacterium glutamicum. To enhance the 2,3-BDO production capacity of C. glutamicum, we introduced budA encoding acetolactate decarboxylase from Klebsiella pneumoniae, a powerful 2,3-BDO producer. Additionally, budB (encoding α-acetolactate synthase) and budC (encoding acetoin reductase) were introduced from K. pneumoniae to reinforce the carbon flux in the 2,3-BDO production. Because budC had a negative effect on 2,3-BDO production in C. glutamicum, the budB and budA introduced strain, SGSC102, was selected for 2,3-BDO production, and batch culture was performed at 30 °C, 250 rpm and pH 6.86 with pure glucose, molasses, and cassava powder as carbon substrates. After batch culture, significant amount of 2,3-BDO (18.9 and 12.0 g/L, respectively) was produced from 80 g/L of pure glucose and cassava powder.
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Acknowledgments
This work was supported by the R&D Program of MOTIE/KEIT (No. 10035578, Development of 2,3-Butanediol and Derivative Production Technology for C-Zero Bio-Platform Industry). This work was also supported by the Graduate School of Specialization for Biotechnology Program of the Ministry of Knowledge Economy.
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Yang, J., Kim, B., Kim, H. et al. Industrial Production of 2,3-Butanediol from the Engineered Corynebacterium glutamicum . Appl Biochem Biotechnol 176, 2303–2313 (2015). https://doi.org/10.1007/s12010-015-1719-7
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DOI: https://doi.org/10.1007/s12010-015-1719-7