Abstract
2,3-Butanediol is an important bio-based chemical product, because it can be converted into several C4 industrial chemicals. In this study, a lactate dehydrogenase-deleted mutant was constructed to improve 2,3-butanediol productivity in Enterobacter aerogenes. To delete the gene encoding lactate dehydrogenase, λ Red recombination method was successfully adapted for E. aerogenes. The resulting strain produced a very small amount of lactate and 16.7% more 2,3-butanediol than that of the wild-type strain in batch fermentation. The mutant and its parental strain were then cultured with six different carbon sources, and the mutant showed higher carbon source consumption and microbial growth rates in all media. The 2,3-butanediol titer reached 69.5 g/l in 54 h during fed-batch fermentation with the mutant, which was 27.4% higher than that with the parental strain. With further optimization of the medium and aeration conditions, 118.05 g/l 2,3-butanediol was produced in 54 h during fed-batch fermentation with the mutant. This is by far the highest titer of 2,3-butanediol with E. aerogenes achieved by metabolic pathway engineering.
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Acknowledgements
This research was supported by the R&D Program of MKE/KEIT (No. 10035578, Development of 2,3-butanediol and derivative production technology for C-Zero bio-platform industry). The authors would also like to thank Macrogen, Inc. for providing the E. aerogenes KCTC2190 full genome sequence.
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Jung, MY., Ng, C.Y., Song, H. et al. Deletion of lactate dehydrogenase in Enterobacter aerogenes to enhance 2,3-butanediol production. Appl Microbiol Biotechnol 95, 461–469 (2012). https://doi.org/10.1007/s00253-012-3883-9
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DOI: https://doi.org/10.1007/s00253-012-3883-9