Abstract
Biosurfactants are amphiphilic molecules having hydrophobic and hydrophilic moieties produced by various microorganisms. These molecules trigger the reduction of surface tension or interfacial tension in liquids. A biosurfactant-producing halophile was isolated from Lake Chilika, a brackish water lake of Odisha, India (19°41′39″N, 85°18′24″E). The halophile was identified as Bacillus tequilensis CH by biochemical tests and 16S rRNA gene sequencing and assigned accession no. KC851857 by GenBank. The biosurfactant produced by B. tequilensis CH was partially characterized as a lipopeptide using thin-layer chromatography, Fourier transform infrared spectroscopy, and nuclear magnetic resonance techniques. The minimum effective concentration of a biosurfactant for inhibition of pathogenic biofilm (Escherichia coli and Streptococcus mutans) on hydrophilic and hydrophobic surfaces was found to be 50 μg ml−1. This finding has potential for a variety of applications.
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One of the authors (AKP) would like to express his sincere thanks to the Council of Scientific and Industrial Research (CSIR), India, for the award of Senior Research Fellowship. The authors are thankful to Eepsita Priyadarshini and Jacintha Esther for editorial assistance in manuscript preparation.
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Pradhan, A.K., Pradhan, N., Mall, G. et al. Application of Lipopeptide Biosurfactant Isolated from a Halophile: Bacillus tequilensis CH for Inhibition of Biofilm. Appl Biochem Biotechnol 171, 1362–1375 (2013). https://doi.org/10.1007/s12010-013-0428-3
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DOI: https://doi.org/10.1007/s12010-013-0428-3