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Anthocyanins During Baking: Their Degradation Kinetics and Impacts on Color and Antioxidant Capacity of Bread

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Abstract

Anthocyanin-rich black rice powder was incorporated into bread, and the stability of two specific anthocyanins, cyanidin-3-glucoside and cyanidin-3-rutinoside, during baking was investigated. Various baking conditions included different baking temperatures (200, 220, and 240 °C) and baking durations (0, 2, 4, 6, 8, 10, and 12 min). Non-isothermal kinetic models were successfully established for the two anthocyanins in both bread crumb and crust. The derived degradation rate (k ref) of cyanidin-3-glucoside and cyanidin-3-rutinoside in bread crumb at the reference temperature (T ref) of 65 °C were 2.49 × 10−5 and 1.93 × 10−5 s−1, respectively. The k ref values of cyanidin-3-glucoside and cyanidin-3-rutinoside in bread crust (T ref = 125 °C) were 5.37 × 10−4 and 5.72 × 10−4 s−1, respectively. The color development of bread crust and crumb was measured and expressed as L*C*H ° values. While the color of bread crust was significantly subjected to variations in oven operating parameters, the color of bread crumb was relatively less affected by baking conditions. The antioxidant capacity and total phenolic content of bread samples were measured using DPPH and Folin-Ciocalteu assays, respectively. Results showed that in bread crumb, both the antioxidant capacity and the total phenolic content decreased; however, an increase in both was observed in bread crust.

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References

  • AOAC. (2000). Official methods of analysis of AOAC international (17th ed.). Gaithersburg: AOAC International.

    Google Scholar 

  • Brand-Williams, W., Cuvelier, M. E., & Berset, C. (1995). Use of a free radical method to evaluate antioxidant activity. LWT - Food Science and Technology, 28, 25–30.

    Article  CAS  Google Scholar 

  • Bueno, J. M., Saez-Plaza, P., Ramos-Escudero, F., Jimenez, A. M., Fett, R., & Asuero, A. G. (2012). Analysis and antioxidant capacity of anthocyanin pigments. Part II: chemical structure, color, and intake of anthocyanins. Critical Reviews in Analytical Chemistry, 42, 126–151.

    Article  CAS  Google Scholar 

  • Cavalcanti, R. N., Santos, D. T., & Meireles, M. A. A. (2011). Non-thermal stabilization mechanisms of anthocyanins in model and food systems—an overview. Food Research International, 44, 499–509.

    Article  CAS  Google Scholar 

  • Chun, O. K., Chung, S. J., & Song, W. O. (2007). Estimated dietary flavonoid intakes and major food sources of U.S. adults. Journal of Nutrition, 137, 1244–1252.

    CAS  Google Scholar 

  • Eliasson, A.-C. (2003). Utilization of thermal properties for understanding baking and staling processes. In G. Kaletunc & K. J. Breslauer (Eds.), Characterization of cereals and flours: properties, analysis and applications (pp. 65–66). New York: Marcel Dekker.

    Google Scholar 

  • Fernandes, I., Marques, F., de Freitas, V., & Mateus, N. (2013). Antioxidant and antiproliferative properties of methylated metabolites of anthocyanins. Food Chemistry, 141, 2923–2933.

    Article  CAS  Google Scholar 

  • Feyissa, A. H., Gernaey, K. V., Ashokkumar, S., & Adler-Nissen, J. (2011). Modelling of coupled heat and mass transfer during a contact baking process. Journal of Food Engineering, 106, 228–235.

    Article  Google Scholar 

  • Fleschhut, J., Kratzer, F., Rechkemmer, G., & Kulling, S. E. (2006). Stability and biotransformation of various dietary anthocyanins in vitro. European Journal of Nutrition, 45, 7–18.

    Article  CAS  Google Scholar 

  • Harbourne, N., Jacquier, J. C., Morgan, D. J., & Lyng, J. G. (2008). Determination of the degradation kinetics of anthocyanins in a model juice system using isothermal and non-isothermal methods. Food Chemistry, 111, 204–208.

    Article  CAS  Google Scholar 

  • Hendry, G. A. F., & Houghton, J. D. (1996). Anthocyanins and betalains. In G. A. F. Hendry & J. D. Houghton (Eds.), Natural food colorants (2nd ed., p. 261). Glasgow: Blackie Academic and Professional.

    Chapter  Google Scholar 

  • Hernández-Herrero, J. A., & Frutos, M. J. (2014a). Effect of concentrated plum juice on physicochemical and sensory properties of yoghurt made at bench-top scale. International Journal o f Diary Technology, 67, 123–128.

    Article  Google Scholar 

  • Hernández-Herrero, J. A., & Frutos, M. J. (2014b). Color and antioxidant capacity stability in grape, strawberry and plum peel model juices at different pHs and temperatures. Food Chemistry, 154, 199–204.

    Article  Google Scholar 

  • Holtekjølen, A. K., Bævre, A. B., Rødbotten, M., Berg, H., & Knutsen, S. H. (2008). Antioxidant properties and sensory profiles of breads containing barley flour. Food Chemistry, 110, 414–421.

    Article  Google Scholar 

  • Homann, J.-P. (2009). Color theory with realistic colors. In J.-P. Homann (Ed.), Digital color management: principles and strategies for the standardized print production (p. 44). Berlin: Springer.

    Google Scholar 

  • Jiménez, N., Bohuon, P., Dornier, M., Bonazzi, C., Pérez, A. M., & Vaillant, F. (2012). Effect of water activity on anthocyanin degradation and browning kinetics at high temperatures (100–140 °C). Food Research International, 47, 106–115.

    Article  Google Scholar 

  • Kırca, A., Özkan, M., & Cemeroğlu, B. (2007). Effects of temperature, solid content and pH on the stability of black carrot anthocyanins. Food Chemistry, 101, 212–218.

    Article  Google Scholar 

  • Konczak, I., & Zhang, W. (2004). Anthocyanins—more than nature’s colors. Journal of Biomedicine and Biotechnology, 5, 239–240.

    Article  Google Scholar 

  • Lee, J. H. (2010). Identification and quantification of anthocyanins from the grains of black rice (Oryza sativa L.) varieties. Food Science and Biotechnology, 19, 391–397.

    Article  CAS  Google Scholar 

  • NPCS Board of Consultants and Engineers. (2011). Handbook on fermented foods and chemicals. Delhi: Asia Pacific Business Press.

    Google Scholar 

  • Patras, A., Brunton, N. P., O’Donnell, C., & Tiwari, B. K. (2010). Effect of thermal processing on anthocyanin stability in foods; mechanisms and kinetics of degradation. Trends in Food Science and Technology, 21, 3–11.

    Article  CAS  Google Scholar 

  • Peng, X., Ma, J., Cheng, K.-W., Jiang, Y., Chen, F., & Wang, M. (2010). The effects of grape seed extract fortification on the antioxidant activity and quality attributes of bread. Food Chemistry, 119, 49–53.

    Article  CAS  Google Scholar 

  • Purlis, E., & Salvadori, V. O. (2007). Bread browning kinetics during baking. Journal of Food Engineering, 80, 1107–1115.

    Article  CAS  Google Scholar 

  • Purlis, E., & Salvadori, V. O. (2009). Modelling the browning of bread during baking. Food Research International, 42, 865–870.

    Article  CAS  Google Scholar 

  • Purlis, E., & Salvadori, V. O. (2010). A moving boundary problem in a food material undergoing volume change—simulation of bread baking. Food Research International, 43, 949–958.

    Article  Google Scholar 

  • Reyes, L. F., & Cisneros-Zevallos, L. (2007). Degradation kinetics and color of anthocyanins in aqueous extracts of purple- and red-flesh potatoes (Solanum tuberosum L.). Food Chemistry, 100, 885–894.

    Article  CAS  Google Scholar 

  • Rodriguez-Mateos, A., Cifuentes-Gomez, T., George, T. W., & Spencer, J. P. E. (2013). Impact of cooking, proving, and baking on the (poly)phenol content of wild blueberry. Journal of Agricultural and Food Chemistry. doi:10.1021/jf403366q.

    Google Scholar 

  • Rodriguez-Saona, L. E., & Wrolstad, R. E. (2001). Extraction, isolation, and purification of anthocyanins. In R. E. Wrolstad (Ed.), Current protocols in food analytical chemistry. New York: Wiley.

    Google Scholar 

  • Rubinskiene, M., Jasutiene, I., Venskutonis, P. R., & Viskelis, P. (2005). HPLC determination of the composition and stability of blackcurrant anthocyanins. Journal of Chromatographic Science, 43, 478–482.

    Article  CAS  Google Scholar 

  • Ryckaert, V. G., Claes, J. E., & Van Impe, J. F. (1999). Model-based temperature control in ovens. Journal of Food Engineering, 39, 47–58.

    Article  Google Scholar 

  • Sharma, A., & Zhou, W. (2011). A stability study of green tea catechins during the biscuit making process. Food Chemistry, 126, 568–573.

    Article  CAS  Google Scholar 

  • Shibukawa, S., Sugiyama, K., & Yano, T. (1989). Effects of heat transfer by radiation and convection on browning of cookies at baking. Journal of Food Science, 54, 621–624.

    Article  Google Scholar 

  • Shipp, J., & Abdel-Aal, E.-S. M. (2010). Food applications and physiological effects of anthocyanins as functional food ingredients. Open Food Science Journal, 4, 7–22.

    Article  CAS  Google Scholar 

  • Slavin, M., Lu, Y., Kaplan, N., & Yu, L. (2013). Effects of baking on cyanidin-3-glucoside content and antioxidant properties of black and yellow soybean crackers. Food Chemistry, 141, 1166–1174.

    Article  CAS  Google Scholar 

  • Sólyom, K., Solá, R., Cocero, M. J., & Mato, R. B. (2014). Thermal degradation of grape marc polyphenols. Food Chemistry, 159, 361–366.

    Article  Google Scholar 

  • Sui, X., & Zhou, W. (2014). Monte Carlo modelling of non-isothermal degradation of two cyanidin-based anthocyanins in aqueous system at high temperatures and its impact on antioxidant capacities. Food Chemistry, 148, 342–350.

    Article  CAS  Google Scholar 

  • Sui, X., Dong, X., & Zhou, W. (2014). Combined effect of pH and high temperature on the stability and antioxidant capacity of two anthocyanins in aqueous solution. Food Chemistry, 163, 163–170.

    Article  CAS  Google Scholar 

  • Tan, M. Y., & Zhou, W. (2009). Modelling of bread colour development during baking. Food Manufacturing Efficiency, 2(2), 9–15.

    Article  Google Scholar 

  • Therdthai, N., & Zhou, W. (2003). Recent advances in the studies of bread baking process and their impacts on the bread baking technology. Food Science and Technology Research, 9, 219–226.

    Article  Google Scholar 

  • Tsuda, T. (2012). Dietary anthocyanin-rich plants: biochemical basis and recent progress in health benefits studies. Molecular Nutrition and Food Research, 56, 159–170.

    Article  CAS  Google Scholar 

  • Vera de Rosso, V., & Mercadante, A. Z. (2007). Evaluation of color and stability of anthocyanins from tropical fruits in an isotonic soft drink system. Innovative Food Science and Emerging Technologies, 8, 347–352.

    Article  Google Scholar 

  • Vhangani, L. N., & Van Wyk, J. (2013). Antioxidant activity of Maillard reaction products (MRPs) derived from fructose-lysine and ribose-lysine model systems. Food Chemistry, 137, 92–98.

    Article  CAS  Google Scholar 

  • Wang, W.-D., & Xu, S.-Y. (2007). Degradation kinetics of anthocyanins in blackberry juice and concentrate. Journal of Food Engineering, 82, 271–275.

    Article  CAS  Google Scholar 

  • Wang, R., & Zhou, W. (2004). Stability of tea catechins in the breadmaking process. Journal of Agricultural and Food Chemistry, 52, 8224–8229.

    Article  CAS  Google Scholar 

  • Wang, R., Zhou, W., & Jiang, X. (2008). Mathematical modeling of the stability of green tea catechin epigallocatechin gallate (EGCG) during bread baking. Journal of Food Engineering, 87, 505–513.

    Article  CAS  Google Scholar 

  • Waterhouse, A. L. (2001). Determination of total phenolics. In R. E. Wrolstad (Ed.), Current protocols in food analytical chemistry. New York: Wiley.

    Google Scholar 

  • Wiczkowski, W., Topolska, J., & Honke, J. (2014). Anthocyanins profile and antioxidant capacity of red cabbages are influenced by genotype and vegetation period. Journal of Functional Foods, 7, 201–211.

    Article  CAS  Google Scholar 

  • Yang, Z., Han, Y., Gu, Z., Fan, G., & Chen, Z. (2008). Thermal degradation kinetics of aqueous anthocyanins and visual color of purple corn (Zea mays L.) cob. Innovative Food Science and Emerging Technologies, 9, 341–347.

    Article  CAS  Google Scholar 

  • Zhou, W., & Therdthai, N. (2007). Three-dimensional CFD modeling of a continuous industrial baking processes. In D.-W. Sun (Ed.), Computational fluid dynamics in food processing (pp. 288–289). Boca Raton: CRC Press.

    Google Scholar 

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Acknowledgments

Support from A*STAR through research grant SERC 112 177 0038 and the National University of Singapore (Suzhou) Research Institute under the grant number NUSRI2011-007 is gratefully acknowledged. The first author also likes to thank China Scholarship Council (CSC) and the National University of Singapore (NUS) for financial support.

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  1. Food Science and Technology Programme, c/o Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore, 117546, Singapore

    Xiaonan Sui, Pei Yi Yap & Weibiao Zhou

  2. National University of Singapore (Suzhou) Research Institute, 377 Linquan Street, Suzhou Industrial Park, Jiangsu, 215123, People’s Republic of China

    Weibiao Zhou

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  1. Xiaonan Sui
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Correspondence to Weibiao Zhou.

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Sui, X., Yap, P.Y. & Zhou, W. Anthocyanins During Baking: Their Degradation Kinetics and Impacts on Color and Antioxidant Capacity of Bread. Food Bioprocess Technol 8, 983–994 (2015). https://doi.org/10.1007/s11947-014-1464-x

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