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Identification and quantification of anthocyanins from the grains of black rice (Oryza sativa L.) varieties

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Abstract

The purpose of this research was conducted to determine anthocyanin content from the grains of 10 Korean black rice varieties. Moreover, the primary constituents including protein and oil were evaluated. Anthocyanins, cyanidin-3-O-glucoside (1) and peonidin-3-O-glucoside (2), were isolated and elucidated using reversephase C18 chromatography, nuclear magnetic resonance (NMR) spectroscopy, and high performance liquid chromatography (HPLC) with diode array detection and electrospray ionization/mass spectrometry (DAD-ESI/MS). Anthocyanin showed significant differences and cyanidin-3-O-glucoside (1) (52.1±6.3-1,601.0±8.5 μg/g) exhibited a markedly higher content than peonidin-3-O-glucoside (2) (ND-82.6±1.2 μg/g). Among varieties, ‘Heugjinjubyeo’ showed the highest anthocyanin (1: 1,601.0±8.5, 2: 82.6±1.2 μg/g), whereas ‘Heughyang’ was the lowest (1: 52.1±6.3 μg/g, 2: ND). Protein and oil exhibited the minor differences and their contents ranged from 10.7±0.0 to 14.1±0.1% and from 2.1±0.0 to 2.9±0.0%. Overall results suggest that anthocyanin can be a key factor in functional property of black rice and ‘Heugjinjubyeo’ may be very important source concerning nutritional value.

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References

  1. Gregorio GB, Senadhira D, Htut T, Graham RD. Improving iron an zinc value of rice for human nutrients. Agr. Develop. 23: 68–87 (1999)

    Google Scholar 

  2. Tsuda T, Watanabe M, Ohshima K, Norinobu S, Choi SW, Kawakishi S, Osawa T. Antioxidative activity of the anthocyanin pigments cyanidin-3-O-β-d-glucoside and cyanidin. J. Agr. Food Chem. 42: 2407–2410 (1994)

    Article  CAS  Google Scholar 

  3. Chung YM, Lee JC, Kim KS, Eun JB. Chemical compositions of 26 varieties of Korean rice straw. Food Sci. Biotechnol. 10: 267–271 (2001)

    Google Scholar 

  4. Lee YR, Kim JY, Woo KS, Hwang IG, Kim KH, Kim KJ, Kim JH, Jeong HS. Changes in the chemical and functional components of Korean rough rice before and after germination. Food Sci. Biotechnol. 16: 1006–1010 (2007)

    CAS  Google Scholar 

  5. Chung HS, Woo WS. A quinolone alkaloid with antioxidant activity from the aleurone layer of anthocyanin-pigmented rice. J. Nat. Prod. 64: 1579–1580 (2001)

    Article  CAS  Google Scholar 

  6. Lee HJ, Oh SK, Choi HC, Kim SU. Identification of anthocyanins from pigmented rice seed. Agric. Chem. Biotechnol. 41: 257–265 (1988)

    Google Scholar 

  7. Yoshinaga K, Yakahashi K, Yoshizawa K. Liquor with pigments of red rice. J. Brew. Soc. Jpn. 81: 337–342 (1986)

    CAS  Google Scholar 

  8. Ling WH, Wang LL, Ma J. Supplementation of the black rice outer fraction to rabbits decrease atherosclerotic plaque formation and increase antioxidant status. J. Nutr. 132: 20–26 (2002)

    CAS  Google Scholar 

  9. Hu C, Zawistowski J, Ling W, Kitts DD. Black rice (Oryza sativa L. indica) pigmented fraction suppresses both reactive oxygen species and nitric oxide in chemical and biological model systems. J. Agr. Food Chem. 51: 5271–5277 (2003)

    Article  CAS  Google Scholar 

  10. Middleton Jr E, Kandaswami C, Theoharides TC. The effects of plant flavonoids on mammalian cells: Implications for inflammation, heart disease, and cancer. Pharmacol. Rev. 52: 673–751 (2000)

    CAS  Google Scholar 

  11. Jang M, Cai L, Udeani GO, Slowing KV, Thomas CF, Beecher CW, Fong HH, Farnsworth NR, Kinghorn AD, Mehta RC, Moon RC, Pezzuto. Cancer chemopreventive activity of resveratrol, a natural product derived from grapes. Science 275: 218–220 (1997)

    Article  CAS  Google Scholar 

  12. Shim SI, Chung JW, Lee JM, Hwang KT, Sone J, Hong BS, Cho HY, Jun WJ. Hepatopotective effects of black rice on superoxide anion radicals in HepG2 Cells. Food Sci. Biotechnol. 15: 993–996 (2006)

    CAS  Google Scholar 

  13. Xia M, Ling WH, Ma J. Kitts DD, Zawistowski J. Supplementation of diets with the black rice pigment fraction attenuates atherosclerotic plaque formation in apolipoprotein E deficient mice. J. Nutr. 133: 744–751 (2002)

    Google Scholar 

  14. Chiang AN, Wu HL, Yeh HI, Chu CS, Lin HC, Lee WC. Antioxidant effects of black rice extract through the induction of superoxide dismutase and catalase activities. Lipids 41: 797–803 (2006)

    Article  CAS  Google Scholar 

  15. Choi YM, Jeong HS, Lee JS. Antioxidant activity of methanolic extracts from some grains consumed in Korea. Food Chem. 103: 130–138 (2007)

    Article  CAS  Google Scholar 

  16. Serrewaltthanawut I, Prapintip S, Watchiraruji K, Goto M, Sasaki M, Shotipru K. Extraction of protein and amino acids from deolled rice bran by subcritical water hydrolysis. Bioresource Technol. 99: 555–561 (2008)

    Article  Google Scholar 

  17. Wei W, Fang-min C, Zheng-hui L, Ke-su W. Differences of phytic acid content and its relation to four protein composition contents in grains of twenty-nine japonica rice varieties from Jiangsu and Zhejiang provinces, China Rice Sci. 14: 311–314 (2007)

    Article  Google Scholar 

  18. Vogel RA, Corretti MC, Plotnick GD. The postprandial effect of components of the Mediterranean diet on endothelial function. J. Am. Coll. Cardiol. 36: 1455–1460 (2000)

    Article  CAS  Google Scholar 

  19. Marchesi S, Roscini AR, Lupattelli G, Siepi D, Pasqualini L, Pirro M, Vaudo G, Mannarino E. Postprandial impairment of brachial flow-mediated vasodilation after an oral fat load constituted by rice oil. Nutr. Res. 22: 1003–1007 (2002)

    Article  CAS  Google Scholar 

  20. Kim SL, Berhow MA, Kim JT, Chi HY, Lee SJ, Chung IM. Evaluation of soyasaponin, isoflavone, protein, lipid, and free sugar accumulation in developing soybean seeds. J. Agr. Food Chem. 54: 10003–10010 (2006)

    Article  CAS  Google Scholar 

  21. Yawadio R, Tanimori S, Morita N. Identification of phenolic compounds isolated from pigmented rices and their aldose reductase inhibitory activities. Food Chem. 101: 1616–1625 (2007)

    Article  CAS  Google Scholar 

  22. Choi SW, Kang WW, Osawa T. Isolation and identification of anthocyanins pigments in black rice. Foods Biotechnol. 3: 131–136 (1994)

    Google Scholar 

  23. Lee JH, Kang NS, Shin SO, Shin SH, Lim SG, Suh DY, Baek IY, Park KY, Ha TJ. Characterisation of anthocyanins in the black soybean (Glycine max L.) by HPLC-DAD-ESI/MS analysis. Food Chem. 112: 226–231 (2009)

    Article  CAS  Google Scholar 

  24. Chandi GK, Sogi DS. Functional properties of rice bran protein concentrations. J. Food Eng. 79: 592–597 (2007)

    Article  CAS  Google Scholar 

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  1. Department of Monitoring and Analysis, NAKDONG River Basin Environmental Office, Ministry of Environment, Changwon, Gyeongnam, 641-722, Korea

    Jin Hwan Lee

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  1. Jin Hwan Lee
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Correspondence to Jin Hwan Lee.

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Lee, J.H. Identification and quantification of anthocyanins from the grains of black rice (Oryza sativa L.) varieties. Food Sci Biotechnol 19, 391–397 (2010). https://doi.org/10.1007/s10068-010-0055-5

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  • DOI: https://doi.org/10.1007/s10068-010-0055-5

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