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Exogenous jasmonic acid modulates the physiology, antioxidant defense and glyoxalase systems in imparting drought stress tolerance in different Brassica species

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Abstract

This study examined the ability of jasmonic acid (JA) to enhance drought tolerance in different Brassica species in terms of physiological parameters, antioxidants defense, and glyoxalase system. Ten-day-old seedlings were exposed to drought (15 % polyethylene glycol, PEG-6000) either alone or in combination with 0.5 mM JA. Drought significantly increased lipoxygenase activity and oxidative stress, levels of malondialdehyde and H2O2. Drought reduced seedling biomass, chlorophyll (chl) content, and leaf relative water content (RWC). Drought increased proline, oxidized ascorbate (DHA) and glutathione disulfide (GSSG) levels. Drought affected different species differently: in B. napus, catalase (CAT) and glyoxalase II (Gly II) activities were decreased, while glutathione-S-transferase (GST) and glutathione peroxidase (GPX) activities were increased in drought-stressed compared to unstressed plants; in B. campestris, activities of glutathione reductase (GR), glyoxalase I (Gly I), GST, and GPX were increased, monodehydroascorbate reductase (MDHAR), dehydroascorbate reductase (DHAR), CAT and other enzymes were decreased; in B. juncea, activities of ascorbate peroxidase, GR, GPX, Gly I were increased; Gly II activity was decreased and other enzymes did not change. Spraying drought-stressed seedlings with JA increased GR and Gly I activities in B. napus; increased MDHAR activity in B. campestris; and increased DHAR, GR, GPX, Gly I and Gly II activities in B. juncea. JA improved fresh weight, chl, RWC in all species, dry weight increased only in B. juncea. Brassica juncea had the lowest oxidative stress under drought, indicating its natural drought tolerance capacity. The JA improved drought tolerance of B. juncea to the highest level among studied species.

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Abbreviations

AO:

Ascorbate oxidase

APX:

Ascorbate peroxidase

BSA:

Bovine serum albumin

CAT:

Catalase

CDNB:

1-Chloro-2,4-dinitrobenzene

chl:

Chlorophyll

DHA:

Dehydroascorbate

DHAR:

Dehydroascorbate reductase

DTNB:

5,5′-Dithio-bis(2-nitrobenzoic acid)

EDTA:

Ethylenediaminetetraacetic acid

Gly I:

Glyoxalase I

Gly II:

Glyoxalase II

GR:

Glutathione reductase

GSH:

Reduced glutathione

GSSG:

Oxidized glutathione

GPX:

Glutathione peroxidase

GST:

Glutathione S-transferase

JA:

Jasmonic acid (JA)

LOX:

Lipoxygenase

MDA:

Malondialdehyde

MDHA:

Monodehydroascorbate

MDHAR:

Monodehydroascorbate reductase

MG:

Methylglyoxal

NADPH:

Nicotinamide adenosine dinucleotide phosphate

NTB:

2-Nitro-5-thiobenzoic acid

PEG:

Polyethylene glycol

Pro:

Proline

ROS:

Reactive oxygen species

RWC:

Relative water content

SLG:

S-d-Lactoylglutathione

TBA:

Thiobarbituric acid

TCA:

Trichloroacetic acid

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Acknowledgments

We are grateful to the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan for financial supports. We acknowledge Dr. Md. Motiar Rohman, Senior Scientific Officer, Bangladesh Agricultural Research Institute for providing Brassica seeds.

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Authors and Affiliations

  1. Laboratory of Plant Stress Responses, Department of Applied Biological Science, Faculty of Agriculture, Kagawa University, Miki-cho, Kita-gun, Kagawa, 761-0795, Japan

    Md. Mahabub Alam, Kamrun Nahar & Masayuki Fujita

  2. Department of Agricultural Botany, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, 1207, Bangladesh

    Kamrun Nahar

  3. Department of Agronomy, Faculty of Agriculture, Sher-e-Bangla Agricultural University, Sher-e-Bangla Nagar, Dhaka, 1207, Bangladesh

    Mirza Hasanuzzaman

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  1. Md. Mahabub Alam
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Correspondence to Masayuki Fujita.

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Alam, M.M., Nahar, K., Hasanuzzaman, M. et al. Exogenous jasmonic acid modulates the physiology, antioxidant defense and glyoxalase systems in imparting drought stress tolerance in different Brassica species. Plant Biotechnol Rep 8, 279–293 (2014). https://doi.org/10.1007/s11816-014-0321-8

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