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Induction of pumpkin glutathione S-transferases by different stresses and its possible mechanisms

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Biologia Plantarum

Abstract

Induction of pumpkin (Cucurbita maxima Duch.) glutathione S-transferases (GSTs) by different stresses and endogenous trans-2-hexenal content were determined in search of a common signal for GST induction. All of the stresses showed significant induction, As2O3 causing the highest induction followed by trans-2-hexenal. The trans-2-hexenal content was highest in trans-2-hexenal-treated seedlings and next-highest in methyl jasmonate-treated seedlings, whereas high temperature- and As2O3-treated seedlings had trans-2-hexenal contents lower than that of control seedlings. Induction of GST, lipoxygenase (LOX) and hydroperoxide lyase (HPL) was compared, since trans-2-hexenal and methyl jasmonate are the products of the LOX pathway. All four stresses showed weak LOX induction, high temperature causing the highest induction. However, only methyl jasmonate caused weak HPL induction. Both antioxidants or oxidants induced GST to different degrees. Glutathione contents of reduced glutathione (GSH) or oxidized glutathione (GSSG)-treated seedlings were significantly higher than the content of control seedlings, whereas those treated with other antioxidants or oxidants had contents similar to or less than control seedlings. The GSH:GSSG ratio was lowest in GSSG-treated seedlings and next-lowest in GSH-treated seedlings. The results of this study suggest that pumpkin GSTs are not induced through a common signalling pathway and that redox perturbation plays a role in pumpkin GST induction.

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Abbreviations

ADH:

alcohol dehydrogenase

DDT:

threo-1,4-dimercapto-2,3-butanediol

EDTA:

ethylenediaminetetraacetic acid

GSH:

reduced glutathione

GSSG:

oxidized glutathione

GST:

glutathione S-transferase

HPL:

hydroperoxide lyase

JA:

jasmonic acid

LOX:

lipoxygenase

MeJA:

methyl jasmonate

NADH:

nicotinamide adenine dinucleotide reduced form

SDS-PAGE:

sodium dodecyl sulfate-polyacrylamide gel electrophoresis

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Authors and Affiliations

  1. Department of Plant Sciences, Faculty of Agriculture, Kagawa University, Ikenobe 2393, Miki-cho, Kagawa, 761-0795, Japan

    M. Z. Hossain, M. D. Hossain & M. Fujita

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  1. M. Z. Hossain
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  2. M. D. Hossain
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  3. M. Fujita
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Correspondence to M. Fujita.

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Hossain, M.Z., Hossain, M.D. & Fujita, M. Induction of pumpkin glutathione S-transferases by different stresses and its possible mechanisms. Biol Plant 50, 210–218 (2006). https://doi.org/10.1007/s10535-006-0009-1

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  • DOI: https://doi.org/10.1007/s10535-006-0009-1

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