Skip to main content
SpringerLink
Log in

Effect of methyl jasmonate treatments on alleviation of polyethylene glycol -mediated water stress in banana (Musa acuminata cv. ‘Berangan’, AAA) shoot tip cultures

  • Original paper
  • Published:
Plant Growth Regulation Aims and scope Submit manuscript

Abstract

The role of exogenous methyl jasmonate (MeJA) application in moderating in vitro water stress on banana growth was investigated. Shoot tips were treated using various levels (0, 5, 10, 20, 40, 80, 100, 120, 140 and 160 μM) of MeJA before and during the imposition of 30 g L−1 PEG induced water stress in the media. Proliferation rate significantly improved with increasing doses of MeJA up to 80 μM whether the shoot tips were stressed by PEG or unstressed. The same trend was observed in terms of enhanced fresh weight increase and shoot vigour rate under water stress. PEG significantly reduced the relative water content (%) of shoot tips by about 35 % as compared with those of unstressed conditions, while MeJA application up to 40 μM reduced the inhibitory effect of PEG on leaf water loss (%). Shoot tips under water stressed conditions, responded positively to MeJA by exhibiting a significant increase in proline, although increasing levels to 100 μM and higher had no effect. MeJA alleviated the effect of PEG—induced loss of chlorophyll, although it had no additional benefit by altering the dosages. Under water stress, MeJA application up to 40 μM was also effective in reducing oxidative injury as indicated by significant reduction in H2O2 and MDA contents of shoot tips; higher dosages exhibited no further advantageous effect. These results suggested the participation of MeJA in improving drought tolerance of banana and moderating oxidative stress leading to enhanced plant performance.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7

Similar content being viewed by others

Abbreviations

BAP:

Benzyl aminopurine

DW:

Dry weights

FW:

Fresh weight

JA:

Jasomonic acid

LSD:

Least significant difference

LWL:

Leaf water loss

MeJA:

Methyl jasmonate

MW:

Molecular weight

MDA:

Malondialdehyde

PEG (M.W. 6000):

Polyethylene glycol

PGR:

Plant growth regulators

ROS:

Reactive oxygen species

RWC:

Relative water contents

TBA:

Thiobarbituric acid

TCA:

Trichloroacetic acid

TW:

Turgid weights

References

  • Aftab T, khan MMA, Idress M, Naeem M, Moinuddin, Hashmi N (2010) Methyl jasmonate counteracts boron toxicity by preventing oxidative stress and regulating antioxidant enzyme activities and artemisinin biosynthesis in Artemisia annua L. Protoplasma 248(3):601–612. doi:10.1007/s00709-010-0218-5

  • Ali MB, Hahn EJ, Paek KY (2007) Methyl Jasmonate and salicylic acid induced oxidative stress and accumulation of phenolics in Panax ginseng bioreactor root suspension cultures. Molecules 12:607–621

    Article  PubMed  CAS  Google Scholar 

  • Anjum SA, Wang L, Farooq M, Khan I, Xue L (2011) Methyl jasmonate-induced alteration in lipid peroxidation, antioxidative defence system and yield in soybean under drought. J Agron Crop Sci 197(4):296–301. doi:10.1111/j.1439-037X.2011.00468.x

    Google Scholar 

  • Bates LS, Waldern RP, Teare ID (1973) Rapid determination of free proline from water stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • Chai TT, Fadzillah NM, Kusnan M, Mahmood M (2005) Water stress—induced oxidative damage and antioxidant responses in micropropagated banana plantlets. Biol Plant 49(1):153–156. doi:10.1007/s00000-005-3156-9

    Article  CAS  Google Scholar 

  • Cheong JJ, Choi YD (2003) Methyl jasmonate as a vital substance in plants. Trends Genet 19(7):409–413. doi:10.1016/S0168-9525(03)00138-0

    Article  PubMed  CAS  Google Scholar 

  • Ding CK, Wang CY, Gross KC, Smith DL (2002) Jasmonate and salicylate induce the expression of pathogenesis-related-proteins genes and increase resistance to chilling injury in tomato fruit. Planta 214:895–901

    Article  PubMed  CAS  Google Scholar 

  • Ebrahim MKH, Ibrahim IA, Emara HA, Komor E (2006) Impact of polyethylene glycol—induced water stress on growth and development of shoot tip cultures from different banana (Musa spp.) cultivars. J Appl Hort 8(1):53–57

    Google Scholar 

  • Fang WC, Kao CH (2001) Inhibition of methyl jasmonate—promoted senescence in rice leaves by a metal chelator, 2, 2′—bipyridine. J Plant Growth Regul 33:87–93. doi:10.1023/A:1017539513518

    Article  CAS  Google Scholar 

  • Farooq M, Wahid A, Kobayashi N, Fujita D, Basra SMA (2009) Plant drought stress: effects, mechanisms and management. Agron Sustain Dev 29(1):185–212. doi:10.1051/agro:2008021

    Article  Google Scholar 

  • Gonzalez-Aguilar GA, Tiznado-Hernandez ME, Zavaleta-Gatica R, Martines-Tellez MA (2004) Methyl jasmonate treatments reduce chilling injury and activate the defense response of guava fruits. Biochem Biophys Res Commun 313(3):694–701

    Article  PubMed  CAS  Google Scholar 

  • Huguet-Robert V, Sulpice R, Lefort C, Maerskalck V, Emery N, Larher FR (2003) The suppression of osmoinduced proline response of Brassica napus L. var Oleifera discs by polyunsaturated fatty acids and methyl—jasmonate. Plant Sci 164:119–127. doi:10.1016/S0168-9452(02)00343-6

    Article  CAS  Google Scholar 

  • Ismail MR, Yusoff MK, Maziah M (2004) Growth, water relations, stomatal conductance and proline concentration in water stressed banana (Musa spp.) plants. Asian J Plant Sci 3(6):709–713

    Article  Google Scholar 

  • Jaleel CA, Riadh K, Gopi R, Manivannan P, Ines J, Al-Juburi HJ, Chang-Xing Z, Hong-Bo S, Panneerselvam R (2009) Antioxidant defense responses: physiological plasticity in higher plants under abiotic constraints. Acta Physiol Plant 31:427–436. doi:10.1007/s11738-009-0275-6

    Article  Google Scholar 

  • Jyothsnakumari G, Sudhakar C (2003) Effects of jasmonic acid on groundnut during early seedling growth. Biol Plant 47:453–456. doi:10.1023/B:BIOP.0000023894.72554.b2

    Article  Google Scholar 

  • Keramat B, Kalantari KM, Arvin MJ (2009) Effects of methyl jasmonate in regulating cadmium induced oxidative stress in soybean plant (Glycine max L.). Afr J Biotech 3(5):240–244

    CAS  Google Scholar 

  • Kim EH, Park SH, Kim JK (2009) Methyl jasmonate triggers loss of grain yield under drought stress. Plant Signal Behav 4(4):348–349

    Article  PubMed  CAS  Google Scholar 

  • Kirnak H, Kaya C, Tas I, Higgs D (2001) The influence of water deficit on vegetative growth, physiology, fruit yield and quality in eggplants. Bull J Plant Physiol 27(3–4):34–46

    Google Scholar 

  • Kovacik J, Klejdus B, Stork F, Hedbavny J, Backor M (2011) Comparison of methyl jasmonate and cadmium effect on selected physiological parameters in Scenedesmus quadricauda (chlorophyta, chlorophyceae). J Phycol 47(5):1044–1049. doi:10.1111/j.1529-8817.2011.01027.x

    Article  CAS  Google Scholar 

  • Mata CG, Lamattina L (2001) Nitric oxide induces stomatal closure and enhances the adaptive plant responses against drought stress. Plant Physiol 126:1196–1204. doi:10.1104/pp.126.3.1196

    Google Scholar 

  • Meir S, Droby S, Davidson H, Alsevia S, Cohen L, Horev B, Philosoph-Hadas S (1998) Suppression of Botrytis rot in cut rose flowers by postharvest application of methyl jasmonate. Postharvest Biol Technol 13:235–243

    Article  CAS  Google Scholar 

  • Michel BE, Kaufmann MR (1973) The osmotic potential of polyethylene glycol 6000. Plant Physiol 51:914–916

    Article  PubMed  CAS  Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497

    Article  CAS  Google Scholar 

  • Norastehnia A, Nojavan-Asghari M (2006) Effects of methyl jasmonate on the enzymatic antioxidant defense system in maize seedling subjected to paraquat. Asian J Plant Sci 5(1):17–23

    Article  CAS  Google Scholar 

  • Parthier B (1991) Jasmonates, new regulators of plant growth and development: many facts and few hypotheses on their actions. Bot Acta 104:446–454

    CAS  Google Scholar 

  • Porra RJ (2002) The chequered history of the development and use of simultaneous equations for the accurate determination of chlorophylls a and b. Photosynth Res 73:149–156

    Article  PubMed  CAS  Google Scholar 

  • Pospisilova J (2003) Participation of phytohormones in the stomatal regulation of gas exchange during water stress. Biol Plant 46:491–506

    Article  CAS  Google Scholar 

  • Rohwer CL, Erwin JE (2008) Horticultural applications of jasmonates: a review. J Hort Sci Biotechnol 83(3):283–304

    CAS  Google Scholar 

  • Sivritepe N, Erturk U, Yerlikaya C, Turkan I, Bor M, Ozdemir F (2008) Response of the cherry rootstock to water stress induced in vitro. Biol Plant 52(3):573–576. doi:10.1007/s10535-008-0114-4

    Article  CAS  Google Scholar 

  • Sotiropoulos TE (2007) Effect of NaCl and CaCl2 on growth and contents of minerals, chlorophyll, proline and sugars in the apple rootstock M4 cultured in vitro. Biol Plant 51:177–180. doi:10.1007/s10535-008-0114-4

    Article  CAS  Google Scholar 

  • Strosse H, Andre E, Sagi L, Swennen R, Panis B (2008) Adventitious shoot formation is not inherent to micropropagation of banana as it is in maize. Plant Cell Tiss Org Cult 95(3):321–332. doi:10.1007/s11240-008-9446-1

    Article  Google Scholar 

  • Turner DW, Fortescue JA, Thomas DS (2007) Environmental physiology of the bananas (Musa spp.). Braz J Plant Physiol 19(4):463–484

    Article  CAS  Google Scholar 

  • Velikova V, Loreto F (2005) On the relationship between isoprene emission and thermo tolerance in Phragmites ausrralis leaves exposed to high temperatures and during the recovery from a heat stress. Plant, Cell Environ 28:318–327

    Article  CAS  Google Scholar 

  • Wang SY (1999) Methyl jasmonate reduces water stress in strawberry. J Plant Growth Regul 18:127–134. doi:10.1007/PL00007060

    Article  PubMed  Google Scholar 

  • Wang CY, Buta JG (1994) Methyl jasmonate reduces chilling injury in Cucurbita pepo through its regulation of abscisic acid and polyamine levels. Environ Exp Bot 34(4):427–432 http://dx.doi.org/10.1016/0098-8472 (94)90025-6

  • Wang F, Zeng B, Sun Z, Zhu C (2009) Relationship between proline and Hg+2—induced oxidative stress in tolerant rice mutant. Arch Environ Contam Toxicol 56(4):723–731. doi:10.1007/s00244-008-9226-2

    Article  PubMed  CAS  Google Scholar 

  • Wasternack C, Parthier B (1997) Jasmonate—signaled plant gene expression. Trends Plant Sci 2:302–307

    Article  Google Scholar 

  • Xing H, Tan L, An L, Zhao Z, Wang S, Zhang C (2004) Evidence for the involvement of nitric oxide and reactive oxygen species in osmotic stress tolerance of wheat seedlings: inverse correlation between leaf abscisic acid accumulation and leaf water loss. Plant Growth Reg 42:61–68. doi:10.1023/B:GROW.0000014894.48683.1b

    Article  CAS  Google Scholar 

  • Yoon JY, Hamayun M, Lee SK, Lee IJ (2009) Methyl jasmonate alleviating salinity stress in soybean. J Crop Sci Biotech 12(2):63–68. doi:10.1007/s12892-009-0060-5

    Article  Google Scholar 

Download references

Acknowledgments

The authors acknowledge the financial support of Research University Grant Scheme (RUGS)—UPM, University Putra Malaysia for the project.

Author information

Authors and Affiliations

  1. Department of Horticulture, College of Agriculture, Isfahan University of Technology, IUT, 84156-83111, Isfahan, Iran

    Siamak Shirani Bidabadi & Cyrus Ghobadi

  2. Institute of Tropical Agriculture, University Putra Malaysia, 43400, Serdang, Selangor DE, Malaysia

    Maziah Mahmood & Siamak Shirani Bidabadi

  3. Department of Biochemistry, Faculty of Biotechnology and Molecular Sciences, University Putra Malaysia, 43400, Serdang, Selangor DE, Malaysia

    Maziah Mahmood

  4. Mid-Florida Research and Education Center, Institute of Food and Agricultural Sciences, University of Florida, 2725 Binion Road, Apopka, FL, 32703-8504, USA

    Dennis J. Gray

Authors
  1. Maziah Mahmood
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Siamak Shirani Bidabadi
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Cyrus Ghobadi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Dennis J. Gray
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Siamak Shirani Bidabadi.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Mahmood, M., Bidabadi, S.S., Ghobadi, C. et al. Effect of methyl jasmonate treatments on alleviation of polyethylene glycol -mediated water stress in banana (Musa acuminata cv. ‘Berangan’, AAA) shoot tip cultures. Plant Growth Regul 68, 161–169 (2012). https://doi.org/10.1007/s10725-012-9702-6

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10725-012-9702-6

Keywords

Search

Navigation