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CAT and MDH improve the germination and alleviate the oxidative stress of cryopreserved Paeonia and Magnolia pollen

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Abstract

Researches have reported that reactive oxygen species (ROS)-induced oxidative stress plays an important role in cell cryodamage during cryopreservation. In the current study, pollen from Magnolia denudata and Paeonia lactiflora ‘Zi Feng Chao Yang’ was cryopreserved and incubated with exogenous catalase (CAT) and malate dehydrogenase (MDH) immediately after thawing. The effect of CAT and MDH on the germination of cryopreserved pollen was measured. Based on that, the ROS level, lipid peroxidation and antioxidants activities in fresh pollen, cryopreserved pollen added with or without CAT or MDH were determined to investigate their relationship with oxidative stress. Pollen from Magnolia and Paeonia showed a significant loss of germination, but marked increase of ROS and malondialdehyde (MDA) production after cryostorage. Antioxidant profiles in them were also enhanced. CAT and MDH addition increased the post-LN pollen germination of Magnolia and Paeonia significantly. Their germination rate achieved the highest with 100 IU ml−1 MDH and 400 IU ml−1 CAT application, respectively. Compared to their untreated controls, ROS and MDA accumulation reduced significantly in cryopreserved Magnolia pollen treated with 100 IU ml−1 MDH, while superoxide dismutase (SOD) activity improved markedly. In the case of Paeonia, significantly lower level of ROS and MDA, but higher activity of CAT and SOD were observed in cryopreserved pollen treated with 400 IU ml−1 CAT. In conclusion, pollen deterioration after cryopreservation is associated with ROS-induced oxidative stress. Exogenous CAT and MDH can reduce the oxidative damage through the activity stimulation of antioxidant enzymes, and play a protective role in the pollen during cryopreservation.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (No. 31370693).

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Author notes

  1. Meng Xue Jia and Xue Ru Jiang contributed equally to this work.

Authors and Affiliations

  1. Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, People’s Republic of China

    Meng Xue Jia, Xue Ru Jiang, Jin Xu, Wei Di, Yin Shi & Yan Liu

  2. College of Architecture and Urban Planning, Guangzhou University, Guangzhou, 510006, People’s Republic of China

    Jin Xu

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  1. Meng Xue Jia
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  2. Xue Ru Jiang
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  3. Jin Xu
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  5. Yin Shi
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  6. Yan Liu
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Correspondence to Yan Liu.

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Communicated by M. Lambardi.

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Jia, M.X., Jiang, X.R., Xu, J. et al. CAT and MDH improve the germination and alleviate the oxidative stress of cryopreserved Paeonia and Magnolia pollen. Acta Physiol Plant 40, 37 (2018). https://doi.org/10.1007/s11738-018-2612-0

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  • DOI: https://doi.org/10.1007/s11738-018-2612-0

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