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Effects of cryopreservation on germinability of olive (Olea europaea L.) pollen

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Abstract

Long term storage of viable pollen is important for bank germplasm constitution to preserve resources that can be used in breeding programs, biotechnologies and genetic engineering. Pollen from 12 olive (Olea europaea L.) cultivars was stored for 1 year in liquid nitrogen at −196°C. The morphology of pollen grains and germination rates on fresh and long term stored pollen were observed. Results on in vitro pollen germinability, both before and after cryopreservation, showed highly significant responses among the 12 cultivars. The relationship between germinability and pollen grain size did not reveal any significant relationships in both treatments. Our findings will contribute to the improvement of olive pollen preservation and lead to find a more efficient method for its long term storage, while retaining its germinability.

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Authors and Affiliations

  1. Department of Agro-Forestry and Environmental Biology and Chemistry, Section of Genetics and Breeding, University of Bari, Bari, Italy

    V. Alba

  2. Institute of Plant Genetics, C.N.R, Via Amendola 165/A, 70126, Bari, Italy

    V. Bisignano & G. B. Polignano

  3. Department of Biology, Plant Protection and Agro-Forestry Biotechnology, University of Basilicata, Potenza, Italy

    E. Alba

  4. Plant Virology Institute, C.N.R, Bari, Italy

    A. De Stradis

Authors
  1. V. Alba
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  2. V. Bisignano
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  3. E. Alba
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  4. A. De Stradis
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  5. G. B. Polignano
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Corresponding author

Correspondence to G. B. Polignano.

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Alba, V., Bisignano, V., Alba, E. et al. Effects of cryopreservation on germinability of olive (Olea europaea L.) pollen. Genet Resour Crop Evol 58, 977–982 (2011). https://doi.org/10.1007/s10722-011-9736-z

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  • DOI: https://doi.org/10.1007/s10722-011-9736-z

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