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ROS-induced oxidative stress is closely related to pollen deterioration following cryopreservation

  • Cryopreservation
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Abstract

Oxidative stress induced by excessive accumulation of reactive oxidative species (ROS) during cryopreservation is thought to be one factor contributing to cryodamage of biological materials. To explore the role of oxidative stress in the cryopreservation of plant pollen, germination, ROS, and malondialdehyde (MDA) levels of pollen from 20 ornamental plant species were compared before and after cryopreservation. The results showed that the germinability of cryopreserved pollen from 13 out of the 20 species was not significantly different from that of fresh pollen (group 1), only one increased significantly, while the other six declined significantly (group 2). The MDA content in cryopreserved pollen from nine species in group 1 showed no significant difference from that of fresh pollen, while four species in group 2 rose significantly. This suggested that pollen viability and MDA levels were negatively correlated. ROS generation in cryopreserved pollen from nine species in group 1 was unchanged compared to fresh pollen, while five species in group 2 increased significantly. This suggested that pollen viability was negatively correlated with ROS generation. Additionally, both ROS and MDA levels in pollen from four species in group 2 increased significantly. In conclusion, pollen from most species possesses some cryostorage tolerance, but some species are severely damaged by cryostorage. Oxidative stress induced by the cryostorage in liquid nitrogen (LN) may be a key factor for the decreased viability in pollen following cryopreservation.

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (no. 31370693).

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Authors and Affiliations

  1. Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Key Laboratory of Ornamental Plants Germplasm Innovation and Molecular Breeding, National Engineering Research Center for Floriculture, College of Landscape Architecture, Beijing Forestry University, Beijing, 100083, People’s Republic of China

    Meng Xue Jia, Yin Shi, Wei Di, Xue Ru Jiang, Jin Xu & Yan Liu

  2. College of Architecture and Urban Planning, Guangzhou University, Guangzhou, 510006, China

    Jin Xu

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  1. Meng Xue Jia
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  2. Yin Shi
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  3. Wei Di
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  4. Xue Ru Jiang
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  5. Jin Xu
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  6. Yan Liu
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Correspondence to Yan Liu.

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Editor: Barbara Reed

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Jia, M.X., Shi, Y., Di, W. et al. ROS-induced oxidative stress is closely related to pollen deterioration following cryopreservation. In Vitro Cell.Dev.Biol.-Plant 53, 433–439 (2017). https://doi.org/10.1007/s11627-017-9844-3

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