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Investigating deleterious effects of ultraviolet (UV) radiations on wheat by a quick method

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Abstract

Increasing air pollution has resulted in ozone depletion, which has led to an increase in the amount of ultraviolet (UV) radiation reaching the surface of earth posing hazardous effects on the plant yield. The chlorophyll a fluorescence transients were recorded in vivo using plant efficiency analyzer and analyzed according to JIP test, which can monitor photosystem II (PSII) behavior. This study aims to evaluate the sensitivity of different components of PSII to UV radiations and the extent of damage caused when wheat plants were exposed to UV radiations for 2 and 4 h. It was observed that functional disconnection of light harvesting complexes from PSII complex, accumulation of inactive reaction center, inactivation of oxygen evolving complex, and thus the linear electron transport process (ET0/CS), were drastically affected by UV radiations. This study will contribute to understanding of the basic photosynthetic mechanisms affected in crop plants due to increased UV radiations. The knowledge obtained will be relevant for environmental and plant scientists to plan strategies to cope with stresses.

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Abbreviations

ABS:

Absorption

Chl a :

Chlorophyll a

CS:

Cross section

DIo :

Dissipation

ET0 :

Electron transport

F 0 :

Initial fluorescence

F m :

Maximum fluorescence

F v :

Variable fluorescence

LHC:

Light harvesting complexes

OEC:

Oxygen evolving complex

OJ, JI, IP:

Phases of fluorescence induction curve

PEA:

Plant efficiency analyzer

PSI:

Photosystem I

PSII:

Photosystem II

PQ:

Plastoquinone

PQH2 :

Plastoquinol

RC:

Reaction center

\(Q_{{{\text{A}}^{ - } }}\) :

Reduced primary quinone acceptor of PS II

Q B :

Secondary quinone acceptor of PS II

TRo :

Trapping

UV:

Ultraviolet

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Acknowledgments

SM thanks University Grant Commission, (UGC), India for Post Doctoral Fellowship for Women (PDFWM-2014-15-GEMAD-23945). AJ thanks DST for the project (DST/RUS/RFBR/P-173). We are also thankful to Prof. R.J. Strasser and Ronaldo Maldonado-Rodriguez for gifting Biolyzer HP 3 Software.

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Authors and Affiliations

  1. School of Life Science, Devi Ahilya University, Indore, 452017, MP, India

    S. Mathur & Anjana Jajoo

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  1. S. Mathur
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  2. Anjana Jajoo
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Correspondence to Anjana Jajoo.

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Communicated by Z. Miszalski.

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Mathur, S., Jajoo, A. Investigating deleterious effects of ultraviolet (UV) radiations on wheat by a quick method. Acta Physiol Plant 37, 121 (2015). https://doi.org/10.1007/s11738-015-1874-z

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  • DOI: https://doi.org/10.1007/s11738-015-1874-z

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