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Fast chlorophyll fluorescence transient and nitrogen fixing ability of chickpea nodulation variants

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Photosynthetica

Abstract

High nodulating (HN) selections of the cultivars ICC 4948 and ICC 5003 had the highest nodule number and nodule dry mass followed by low nodulating (LN) selections of the same cultivar. Both non-nodulating (NN) selections of cv. ICC 4993 and ICC 4918 did not show any nodule. Using N-difference method the HN selection of cv. 1CC 4948 was able to meet 73 % of its demand of N through biological fixation of N2 [P(fix)], while 27 % of N demand was met by uptake from the soil, whereas its LN selection was able to meet only 54 % of its demand of N through biological fixation of N2. Similarly in cv. ICC 5003 HN and LN selections the P(fix) was 76 and 64 %, respectively. Fast chlorophyll (Chl) fluorescence transient data analysis showed that performance index PI(abs) was 62.0 in cv. ICC 4948 HN selection and 44.5 in its respective LN selections. Corresponding values for cv. ICC 5003 were 32.4 and 28.4. In NN selections of ICC 4993 and ICC 4918 it was 12.6 and 30.7, respectively. Structure function index of the plants SFI(abs) and driving force for photosynthesis (DF) were highest in the HN selections followed by LN selections and lowest in the NN selections. The total uptake of N by chickpea plants was significantly and positively correlated with the density of reaction centres ABS/CS0, TR0/CS0, and DI0/CSM, whereas total N uptake by chickpea seeds was significantly positively correlated with N and TR0/CS0. The percentage of P(fix) was highly significantly positively correlated with N, the so-called turnover number which indicates how many times QA has been reduced in the time span from 0 to tFmax and TR0/CS0. Fast Chl a fluorescence measurement can be used as a model system to assess the N fixation ability in chickpea.

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Abbreviations

ABS:

photons absorbed by the antenna pigment chlorophyll

CS:

excited cross section of leaf

dV/dt0 or M0 :

slope at the origin of the fluorescence rise

DFPo :

total driving force for photosynthesis

DI0 :

dissipated excitation energy at time zero

ET:

electron transport flux

FJ :

fluorescence intensity at the J step at 2 ms

F0, FM :

initial and maximum Chl a fluorescence, respectively

FV :

variable fluorescence intensity

F150 :

fluorescence intensity at 150 µs

F300 :

fluorescence intensity at 300 µs

I0 :

the incident photon flux

N:

turn over number

PI:

performance index

PIPo :

linear distance between a point to the control

PS2:

photosystem 2

QA :

primary quinone acceptor of PS2

RC:

reaction centre

SFI:

structure-function-index

SFIPo, SFINo :

subscripts “P” and “N” refer to photosynthesis and non-photosynthesis, respectively

SM :

normalized area

tFmax :

time to reach the maximal fluorescence

TR:

energy trapping flux

VJ :

intermediate step

ϕE0 :

probability that an absorbed photon will move an electron into the electron transport chain beyond QA

ϕP0 :

maximum quantum yield of primary photochemistry

ψ0 :

efficiency with which a trapped exciton can move an electron into the electron transport chain beyond QA

References

  • Bremner, J.M.: Total nitrogen, 2. — In: Black, C.A. (ed.): Methods of Soil Analysis. Pp. 1149–1178. American Society of Agronomy, Madison 1965.

    Google Scholar 

  • Cassman, K.G., Kropff, M.J., Gaaunt, J., Peng, S.: Nitrogen use efficiency of rice reconsidered: What are the key constraints? — Plant Soil 155/156: 359–362, 1993.

    Article  Google Scholar 

  • Dudeja, S.S., Potdukhe, S.R., Namdeo, S.L., Datar, V.V., Vinod Kumar, Tilak, K.V.B.R., Khurana, A.L., Rupela, O.P.: Evaluation of some selected chickpea nodulation variants at five locations in India. — In: Rupela, O.P., Johansen, C., Herridge, D.F. (ed.): Extending Nitrogen Fixation Research to Farmers’ Fields. Pp. 261–276. ICRISAT, Patancheru 1997.

    Google Scholar 

  • Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. — Biochim. biophys. Acta 990: 87–92, 1989.

    Google Scholar 

  • Herridge, D.F., Danso, S.K.A.: Measuring N2 fixation: an overview of methods. — Plant Soil 174: 51–82, 1995.

    Article  Google Scholar 

  • Khurana, A.L., Dudeja, S.S.: Selection of high nodulating lines of chickpea. — Crop Improvement 23: 208–212, 1996.

    Google Scholar 

  • Lichtenthaler, H.K.: Application of Chlorophyll Fluorescence in Photosynthetic Research, Stress Physiology, Hydrobiology and Remote Sensing. — Kluwer Academic Publ., Dordrecht — Boston — London 1988.

    Google Scholar 

  • Misra, A.N., Srivastava, A., Strasser, R.J.: Fast chlorophyll a fluorescence kinetic analysis for the assessment of temperature and light effects: A sliding model for stress recovery phenomena. — In: Proceedings of the 12th International Congress on Photosynthesis. Chapter S3-007. Pp. 1–4. Brisbane 2001.

  • Owens, T.G.: Processing of excitation energy by antenna pigments. — In Baker, N.R. (ed.): Photosynthesis and the Environment. Pp. 1–23. Kluwer Academic Publ., Dordrecht — Boston — London 1996.

    Google Scholar 

  • Peng, S., Cassman, K.G., Kropff, M.J.: Relationship between leaf photosynthesis and nitrogen content of field-grown rice in tropics. — Crop Sci. 35: 1627–1630, 1995a.

    Google Scholar 

  • Peng, S., Garcia, F.V., Laza, R.C., Sanico, A.L., Visperas, R.M., Cassman, K.G.: Increased N-use efficiency using a chlorophyll meter on high-yielding irrigated rice. — Field Crops Res. 47: 243–252, 1996.

    Article  Google Scholar 

  • Peng, S., Laza, R.C., Garcia, F.V., Cassman, K.G.: Chlorophyll meter estimates leaf area-based nitrogen concentration of rice. — Commun. Soil Sci. Plant Anal. 26: 927–935, 1995b.

    Google Scholar 

  • Peoples, M.B., Ladha, J.K., Herridge, D.F.: Enhancing legumes nitrogen fixation through plant and soil management. — Plant Soil 174: 83–101, 1995.

    Article  Google Scholar 

  • Rupela, O.P.: Screening for intracultivaral variability for nodulation of chickpea and pigeonpea. — In: Rupela, O.P., Kumar Rao, J.V.D.K., Wani, S.P., Johansen, C. (ed.): Linking Biological Nitrogen Fixation Research in Asia. Pp. 76–83. ICRISAT, Patancheru 1994.

    Google Scholar 

  • Schmitz, P., Maldonado-Rodriguez, R., Strasser, R.J.: Evaluation of the nodulated status of Vigna unguiculata probed by the JIP-test based on the chlorophyll a fluorescence rise. — In: Proceedings of the 12th International Congress on Photosynthesis. Chapter S36-012. Pp. 1–4. Brisbane 2001.

  • Schreiber, U., Bilger, W., Neubauer, C.: Chlorophyll fluorescence as a non intrusive indicator for rapid assessment of in vivo photosynthesis. — In: Schulze, E.D., Caldwell, M.M. (ed.): Ecophysiology of Photosynthesis. Pp. 49–70. Springer-Verlag, Berlin — Heidelberg — New York 1995.

    Google Scholar 

  • Sheoran, A., Khurana, A.L., Dudeja, S.S.: Nodulation competitiveness in Rhizobium chickpea nodulation variants symbiosis. — Microbiol. Res. 152: 407–412, 1997.

    Google Scholar 

  • Srivastava, A., Strasser, R.J.: Survival strategies of plants to cope with the stress of daily atmospheric changes. — In: Behl, R.K., Punia, M.S., Lather, B.P.S. (ed.): Crop Improvement for Food Security. Pp. 60–71. SSARM, Hisar 1999.

    Google Scholar 

  • Strasser, R.J., Srivastava, A., Govindjee: Polyphasic chlorophyll a fluorescence transient in plants and cyanobacteria. — Photochem. Photobiol. 61: 32–34, 1998.

    Google Scholar 

  • Strasser, R.J., Srivastava, A., Tsimilli-Michael, M.: The fluorescence transient as a tool to characterize and screen photosynthetic samples. — In: Yunus, M., Pathre, U., Mohanty, P. (ed.): Probing Photosynthesis: Mechanisms, Regulation and Adaptation. Pp. 445–483. Taylor & Francis, London — New York 2000.

    Google Scholar 

  • Strasser, R.J., Tsimilli-Michael, M., Greppin, H.: How excitation energy distribution indicates the complexity of a developing and to the environment adapting photosynthetic apparatus. — In: Greppin, H., Penel, C., Simon, P. (ed.): Travelling Shot on Plant Development. Pp. 99–129. Rochat-Baumann, Imprimerie Nationale, Geneve 1997.

    Google Scholar 

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  1. Department of Microbiology, CCS Haryana Agricultural University, Hisar, 125004, India

    S. S. Dudeja & P. Chaudhary

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  1. S. S. Dudeja
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  2. P. Chaudhary
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Dudeja, S.S., Chaudhary, P. Fast chlorophyll fluorescence transient and nitrogen fixing ability of chickpea nodulation variants. Photosynthetica 43, 253–259 (2005). https://doi.org/10.1007/s11099-005-0041-y

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