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Changes in composition of phenolic compounds and antioxidant properties of Vitis amurensis seeds germinated under osmotic stress

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Abstract

The research focused on the changes of phenolic compounds as well as their antiradical activity and reducing power isolated from Amur grape (Vitis amurensis) seeds during germination under optimal conditions and under osmotic stress. The seeds were found to contain tannins, (+) catechin, (−) epicatechin, and gallic acid (in free, ester- and glycoside-bound forms). Extracts from the seeds were also shown to contain two other phenolic acids: caffeic and p-coumaric acids, in very low levels. During a 3-day seed germination test under osmotic stress (−0.5 MPa), the content of total phenolics, tannins and phenolic acids declined as compared to the control. However, seed germination under stress conditions led to a significant increase in the amount of catechins. Because catechin is the one of the units in condensed tannins, its dynamic increase during seed germination may be involved in metabolism of tannins under osmotic stress. It is also likely that the synthesis of catechins is greater under stress conditions and these compounds may be engaged in the process of acclimatization of grapevines to stress conditions. The content of total phenolic compounds in seed extracts is positively correlated with their antioxidant properties. The extracts from seeds germinated under optimal conditions exhibited strong antiradical properties against the DPPH (2,2-diphenyl-1-picrylhydrazyl) radical as well as reducing power. As regards the extracts from grape seeds germinated under osmotic stress, this capability was much weaker. The research demonstrated that antioxidants could interfere with the oxidation process induced by various stresses by acting as oxygen scavengers, therefore the tolerance to drought stress might be correlated with an increase in the antioxidant potential.

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References

  • Alsher RG (1989) Biosynthesis and antioxidant function of glutathione in plants. Physiol Plant 77:457–464

    Article  Google Scholar 

  • Amarowicz R, Weidner S (2001) Content of phenolic acids in rye caryopses determined using DAD-HPLC method. Czech J Food Sci 19:201–203

    CAS  Google Scholar 

  • Amarowicz R, Piskuła M, Honke J, Rudnicka B, Troszyńska A, Kozłowska H (1995) Extraction of phenolic compounds from lentil seeds (Lens culinaris) with various solvents. Pol J Food Nutr Sci 4/45:53–62

    Google Scholar 

  • Amarowicz R, Naczk M, Zadernowski R, Shahidi F (2000) Antioxidant activity of condensed tannins of beach pea, canola hulls, evening primrose, and faba beans. J Food Lipids 7:199–211

    Article  Google Scholar 

  • Amarowicz R, Pegg RB, Rahimi-Moghaddam P, Barl B, Weil JA (2004) Free-radical scavenging capacity and antioxidant activity of selected plant species from the Canadian prairies. Food Chem 84:551–562

    Article  CAS  Google Scholar 

  • Bailly C (2004) Active oxygen species and antioxidants in seed biology. Seed Sci Biol 14:93–107

    Article  CAS  Google Scholar 

  • Bakkalbasi E, Yemis O, Aslanova D (2005) Major flavon-3-ol composition and antioxidant activity of seeds from different grape cultivars grown in Turkey. Eur Food Res Technol 221:792–797

    Article  CAS  Google Scholar 

  • Bartosz G (1997) Oxidative stress in plants. Acta Physiol Plant 19:47–64

    Article  CAS  Google Scholar 

  • Caillet S, Salmieri S, Lacroix M (2006) Evaluation of free radical-scavenging properties of commercial grape phenol extracts by a fast colorimetric method. Food Chem 95:1–8

    Article  CAS  Google Scholar 

  • Couper A, Eley D (1984) Surface tension of polyethylene glycol solutions. J Polym Sci 3:345–349

    Article  Google Scholar 

  • De Freitas VAP, Glories Y, Bourgeois G, Vitry C (1998) Characterisation of oligomeric and polymeric procyanidins from grape seeds by liquid secondary ion mass spectrometry. Phytochem 49:1435–1441

    Article  Google Scholar 

  • Elmaki HB, Babiker EE, El Tinay AH (1999) Changes in chemical composition, grain malting, starch and tannin contents and protein digestibility during germination of Sorghum cultivars. Food Chem 64:331–336

    Article  CAS  Google Scholar 

  • Farrant JM, Bailly C, Leymarie J, Hamman B, Come D, Corbineau F (2004) Wheat seedlings as a model to understand desiccation tolerance and sensitivity. Physiol Plant 120:1–12

    Article  Google Scholar 

  • Fridovich I (1986) Biological effects of the super oxide radical. Arch Biochem Biophys 147:1–11

    Article  Google Scholar 

  • Hagerman A, Butler L (1978) Protein precipitation method for quantitative determination of tannins. J Agric Food Chem 26:809–811

    Article  CAS  Google Scholar 

  • Ibrahim SS, Habiba RA, Shatta AA, Embaby HE (2002) Effect of soaking, germination, cooking and fermentation on antinutritional factors in cowpeas. Nahrung Food 46:92–95

    Article  CAS  Google Scholar 

  • Jackson RS (1994) Wine science. Academic, New York

    Google Scholar 

  • Jiangsu New Medical College (1997) Dictionary of chinese traditional medicine, vol 2. Shanghai People’s Publishing House, Shanghai, pp 2315

  • Karamać M, Buciński A, Pegg RB, Amarowicz R (2005) Antioxidant and antiradical activity of ferulates. Czech J Food Sci 23:64–68

    Google Scholar 

  • Kranner I, Beckett RP, Wornik S, Zorn M, Pfeifhofer HW (2002) Revival of a resurrection plant correlates with its antioxidant status. Plant J 31:13–24

    Article  PubMed  CAS  Google Scholar 

  • Kryger K, Sosulski FW, Hogge L (1982) Free, esterified, and insoluble-bound phenolic acids 1. Extraction and purification procedure. J Agric Food Chem 30:330–334

    Article  Google Scholar 

  • Li S-X (1988) Plant catalogue in Liaoning. Liaoning Science and Technology Publishing House, Shenyang, pp 1:1127

  • Madhujith T, Amarowicz R, Shahidi F (2004) Phenolic antioxidants in beans and their effects on inhibition of radical induced DNA damage. J Am Oil Chem Soc 81:691–696

    Article  CAS  Google Scholar 

  • Mbithi-Mwikya S, Van Camp J, Yiru Y, Huyghebaert A (2000) Nutrient and antinutrient changes in finger millet (Eleusine coracan) during sprouting. Lebensm–Wiss u-Technol 33:9–14

    Article  CAS  Google Scholar 

  • Mubarak AE (2005) Nutritional composition and antinutritional factors of mung bean seeds (Phaseolus aureus) as affected by some home traditional processes. Food Chem 89:489–495

    Article  CAS  Google Scholar 

  • Naczk M, Shahidi F (1989) The effect of methanol–ammonia–water treatment on the content of phenolic acids of canola. Food Chem 31:15–164

    Article  Google Scholar 

  • Navari-Izzo F, Rascio N (1999) Plant response to water deficit conditions. In: Passarakli M (ed) Handbook of plant and crop stress. Marcel Dekker Inc., New York, pp 231–270

    Google Scholar 

  • Negro C, Tommasi L, Miceli A (2003) Phenolic compounds and antioxidant activity from red grape marc extracts. Bioresour Technol 87:41–44

    Article  PubMed  CAS  Google Scholar 

  • Oszmiański J, Bourzeix M (1995) Preparation of catechin and procyanidin standards from hawthorn (Crataegus azarolus L.) and pine (Pine mesogeensis fieschi) barks. Pol J Food Nutr Sci 4/45:89–96

    Google Scholar 

  • Powałka A, Wróbel M, Karamać M, Amarowicz, Frączek E, Weidner S (2004) Extracts of phenolic compounds of three grape varieties — comparison of total phenolics and tannins content, their antiradical activity and reduction power. Grapevine: From Ecophysiology to Molecular Biology. Cost 858 Workshop, April 30–May 1. Monte Verita, Ascona, p 29

  • Price NJ, Van Scoyoc S, Butler LG (1978) A critical evaluation of the vanillic reactions an assay for tannin in sorghum grain. J Agric Food Chem 26:1214–1218

    Article  CAS  Google Scholar 

  • Seel WE, Hendry GAF, Atherton NM, Lee JA (1991) Radical formation and accumulation in vitro in desiccation tolerant and intolerant mosses. Free Rad Res Comm 15:133–141

    CAS  Google Scholar 

  • Singleton VL (1992) Tannins and the qualities of wines. In: Laks PE, Hemingway RW (eds) Plant polyphenols. Plenum Press, New York, pp 859–880

    Google Scholar 

  • Smirnoff N (1993) The role of active oxygen in the response of plants to water deficit and desiccation. New Phytol 125:27–58

    Article  CAS  Google Scholar 

  • Smith IK, Vierheller TL, Thorne C (1989) Properties and functions of glutathione reductase in plants. Physiol Plant 77:449–456

    Article  CAS  Google Scholar 

  • Weidner S, Amarowicz R, Karamać M, Frączek E (2000) Changes in endogenous phenolic acids during development of Secale cereale caryopses and after dehydration treatment of unripe rye grains. Plant Physiol Biochem 38:595–602

    Article  CAS  Google Scholar 

  • Weidner S, Frączek E, Amarowicz R, Abe S (2001) Alterations in phenolic acids content in developing rye grains in normal environment and during enforced dehydration. Acta Physiol Plant 23:475–482

    Article  CAS  Google Scholar 

  • Weidner S, Krupa U, Amarowicz R, Karamać M, Abe S (2002) Phenolic compounds in embryos of triticale caryopses at different stages of development and maturation in normal environment and after dehydration treatment. Euphytica 126:115–112

    Article  CAS  Google Scholar 

  • Wróbel M, Karamać M, Amarowicz R, Frączek E, Weidner S (2005) Metabolism of phenolic compounds in Vitis riparia seeds during stratification and during germination under optimal and low temperature stress conditions. Acta Physiol Plant 27:313–320

    Article  Google Scholar 

  • Yen G-C, Chen H-Y (1995) Antioxidant activity of various tea extracts in relation to their antimutagenicity. J Agric Food Chem 43:27–32

    Article  CAS  Google Scholar 

  • Yildirim HK, Akcay YD, Güvenc U, Altindisli A, Sözmen EY (2005) Antioxidant activities of organic grape, pomace, juice, must, wine and their correlation with phenolic content. Inter J Food Sci Tech 40:133–142

    Article  CAS  Google Scholar 

  • Zadernowski R, Kozłowska H (1983) Phenolic acids in soybean and rapeseed flours. Lebensm Wiss Technol 16:110–114

    CAS  Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Biology, University of Warmia and Mazury in Olsztyn, M. Oczapowskiego St. 1A, 10-957, Olsztyn-Kortowo, Poland

    Stanisław Weidner, Ewa Szypulska & Aleksandra Gołgowska

  2. Division of Food Science, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima St. 10, Box 55, 10-718, Olsztyn 5, Poland

    Magdalena Karamać & Ryszard Amarowicz

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  1. Stanisław Weidner
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  2. Magdalena Karamać
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  3. Ryszard Amarowicz
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  4. Ewa Szypulska
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  5. Aleksandra Gołgowska
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Correspondence to Stanisław Weidner.

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Communicated by E. Davies.

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Weidner, S., Karamać, M., Amarowicz, R. et al. Changes in composition of phenolic compounds and antioxidant properties of Vitis amurensis seeds germinated under osmotic stress. Acta Physiol Plant 29, 283–290 (2007). https://doi.org/10.1007/s11738-007-0035-4

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  • DOI: https://doi.org/10.1007/s11738-007-0035-4

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