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Buyang Huanwu Decoction (补阳还五汤) Attenuates Glial Scar by Downregulating the Expression of Leukemia Inhibitory Factor in Intracerebral Hemorrhagic Rats

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Abstract

Objective

To evaluate the effect of Buyang Huanwu Decoction (补阳还五汤, BYHWD) on glial scar after intracerebral hemorrhage (ICH) and investigate the underlying mechanism.

Methods

Collagenase type VII (0.5 U) was injected stereotaxically into right globus pallidus to induce ICH model. One hundred and twenty Sprague-Dawley rats were randomly divided into 3 groups according to a random number table, including normal group (n=40), ICH model group (n=40) and BYHWD group (n=40), respectively. After ICH, the rats in the BYHWD group were intragastrically administered with BYHWD (4.36 g/kg) once a day for 21 days, while the rats in ICH group were administered with equal volume of distilled water for 21 days, respectively. Double immunolabeling was performed for proliferating cell nuclear antigen (PCNA)+/glial fibrillary acidic protein (GFAP)+ nuclei. The expression of GFAP and leukemia inhibitory factor (LIF) was evaluated by immunohistochemistry and quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR).

Results

The astrocytes with hypertrophied morphology around the hematoma was observed on day 3 after ICH. The number of GFAP positive cells and GFAP mRNA levels increased notably on day 3 and reached the peak on day 14 post-ICH (P<0.01). PCNA+/GFAP+ nuclei were observed around the hematoma and reached the peak on day 14 post-ICH (P<0.01). In addition, LIF-positive astrocytes and LIF mRNA level in the hemorrhagic region increased significantly till day 14 post-ICH (P<0.01). However, BYHWD not only reduced the number of PCNA+/GFAP+ nuclei, but also decreased GFAP and LIF levels (P<0.05).

Conclusions

BYHWD could attenuate ICH-induced glial scar by downregulating the expression of LIF in the rats.

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Author information

Authors and Affiliations

  1. Institute of Neurology, China Three Gorges University, Yichang, Hubei Province, 443003, China

    Xiao Kang, Hua-jun Zhou & Jing-hua Zhou

  2. Department of Neurology, The First College of Clinical Medical Sciences, China Three Gorges University, Yichang, Hubei Province, 443003, China

    Xiao Kang, Hua-jun Zhou & Jing-hua Zhou

  3. Key Laboratory of Cardiovascular and Cerebrovascular Diseases Translational Medicine, China Three Gorges University, Yichang, Hubei Province, 443003, China

    Hua-jun Zhou & Jian Yang

  4. Department of Intensive Care Unit, The First College of Clinical Medical Sciences, China Three Gorges University, Yichang, Hubei Province, 443003, China

    Jian-hua Zhong

  5. Institute of Integrative Medicine, Xiangya Hospital, Central South University, Changsha, 410008, China

    Tao Tang & Han-jin Cui Cui

  6. Medical College of China Three Gorges University, Yichang, Hubei Province, 443002, China

    Zhi-gang Mei

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  1. Xiao Kang
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Corresponding author

Correspondence to Hua-jun Zhou.

Additional information

Supported by the National Natural Science Foundation of China (No. 81202625, 30400581, 30873221 and 81173175), the Project for New Century Excellent Talents (NCET-11-0522), the Hunan Provincial Natural Science Foundation (No. 07JJ5007 and 10JJ2023), the Hubei Provincial Natural Science Foundation (No. 2017CFB468) and the Key Laboratory of Cardiovascular and Cerebrovascular Diseases Translational Medicine (Three Gorges University, No. 2016KXN06)

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Kang, X., Zhou, Hj., Yang, J. et al. Buyang Huanwu Decoction (补阳还五汤) Attenuates Glial Scar by Downregulating the Expression of Leukemia Inhibitory Factor in Intracerebral Hemorrhagic Rats. Chin. J. Integr. Med. 25, 264–269 (2019). https://doi.org/10.1007/s11655-018-2917-7

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  • DOI: https://doi.org/10.1007/s11655-018-2917-7

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