Skip to main content
SpringerLink
Log in

Physiological traits and oxidative stress markers during acclimatization of micropropagated plants from two endangered Plantago species: P. algarbiensis Samp. and P. almogravensis Franco

  • Plant Tissue Culture
  • Published:
In Vitro Cellular & Developmental Biology - Plant Aims and scope Submit manuscript

Abstract

Plantago algarbiensis Samp. and Plantago almogravensis Franco are species endemic to Portugal at risk of global extinction. The aim of this study was to investigate the ex vitro performance of micropropagated P. algarbiensis and P. almogravensis plants in terms of survival, relative water content (RWC), photosynthetic pigment contents, H2O2 accumulation, activities of superoxide dismutase (SOD) and catalase (CAT), lipid peroxidation, and soluble protein content, in comparison with wild-grown plants. Relatively high survival rates and RWC values during the acclimatization process were observed for both species. In P. algarbiensis, the pigment content increased when plantlets were transferred to ex vitro conditions, indicating enhanced light absorption capacity. No significant alterations in H2O2 content, CAT activity, or lipid peroxidation level were observed during acclimatization, but the protein content decreased in plants at the end of the growth chamber and greenhouse stages. When P. almogravensis plantlets were transferred to the ex vitro environment, decreases in the H2O2 content were observed that correlated to increased CAT activity and SOD maintenance, which lead to decreased lipid peroxidation and protein content. It was concluded that micropropagated P. algarbiensis and P. almogravensis plants were able to manage the oxidative stress induced by the in vitro environment and to perform well under ex vitro conditions.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Figure 1.
Figure 2.
Figure 3.

Similar content being viewed by others

References

  • Aebi HE (1983) Catalase. In: Bergmeyer HU (ed) Methods of enzymatic analysis. Verlag Chemie, Weinhern, pp 273–286

    Google Scholar 

  • Ahmed MR, Anis M (2014) Changes in activity of antioxidant enzymes and photosynthetic machinery during acclimatization of micropropagated Cassia alata L. plantlets. In Vitro Cell Dev Biol—Plant 50:601–609

    Article  CAS  Google Scholar 

  • Batková P, Pospíšilová J, Synková H (2008) Production of reactive oxygen species and development of antioxidative systems during in vitro growth and ex vitro transfer. Biol Plant 52:413–422

    Article  Google Scholar 

  • Beauchamp CO, Fridovich I (1971) Superoxide dismutase: improved assays and assays applicable to acrylamide gels. Anal Biochem 44:276–287

    Article  CAS  PubMed  Google Scholar 

  • Bradford M (1976) A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254

    Article  CAS  PubMed  Google Scholar 

  • Branquinho C, Serrano HC, Pinto MJ, Martins-Loução MA (2007) Revisiting the plant hyperaccumulation criteria to rare plants and earth abundant elements. Environ Pollut 146:437–443

    Article  CAS  PubMed  Google Scholar 

  • Carvalho LC, Vilela BJ, Vidigal P, Mullineaux PM, Amâncio S (2006) Activation of the ascorbate-glutathione cycle is an early response of micropropagated Vitis vinifera L. explants transferred to ex vitro. Int J Plant Sci 167:759–770

    Article  CAS  Google Scholar 

  • Carvalho LC, Santos S, Vilela J, Amâncio S (2008) Solanum lycopersicon mill. and Nicotiana benthamiana L. under high light shown distinct responses to anti-oxidative stress. J Plant Physiol 165:1300–1312

  • Chakrabarty D, Datta SK (2008) Micropropagation of gerbera: lipid peroxidation and antioxidant enzyme activities during acclimatization process. Acta Physiol Plant 30:325–331

    Article  CAS  Google Scholar 

  • Desjardins Y, Dubuc J-F, Badr A (2009) In vitro culture of plants: a stressful activity! Acta Hort 812:29–50

    Article  CAS  Google Scholar 

  • Dias MC, Pinto G, Santos C (2011) Acclimatization of micropropagated plantlets induces an antioxidative burst: a case study with Ulmus minor Mill. Photosynthetica 49:259–266

    Article  Google Scholar 

  • Dias MC, Pinto G, Correia CM, Moutinho-Pereira J, Silva S, Santos C (2013) Photosynthetic parameters of Ulmus minor plantlets affected by irradiance during acclimatization. Biol Plant 57:33–40

    Article  CAS  Google Scholar 

  • Faisal M, Anis M (2009) Changes in photosynthetic activity, pigment composition, electrolyte leakage, lipid peroxidation, and antioxidant enzymes during ex vitro establishment of micropropagated Rauvolfia tetraphylla plantlets. Plant Cell Tissue Organ Cult 99:125–132

    Article  CAS  Google Scholar 

  • Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930

    Article  CAS  PubMed  Google Scholar 

  • Gonçalves S, Romano A (2016) The medicinal potential of plants from the genus Plantago (Plantaginaceae). Ind Crop Prod 83:213–226

    Article  Google Scholar 

  • Gonçalves S, Martins N, Romano A (2009) Micropropagation and conservation of endangered species Plantago algarbiensis and P. almogravensis. Biol Plant 53:774–778

    Article  Google Scholar 

  • Gonçalves S, Grevenstuk T, Martins N, Romano A (2015) Antioxidant activity and verbascoside content in extracts from two uninvestigated endemic Plantago spp. Ind Crop Prod 65:198–202

    Article  Google Scholar 

  • Guan QZ, Guo YH, Sui XL, Li W, Zhang ZX (2008) Changes in photosynthetic capacity and antioxidant enzymatic systems in micropropagated Zingiber officinale plantlets during their acclimation. Photosynthetica 46:193–201

    Article  CAS  Google Scholar 

  • Hazarika BN (2006) Morpho-physiological disorders in in vitro culture of plants. Sci Hortic 108:105–120

  • Hodges DM, Delong JM, Forney CF, Prange RK (1999) Improving the thiobarbituric acid-reactive-substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207:604–611

    Article  CAS  Google Scholar 

  • Lichtenthaler HK (1987) Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. Meth Enzymol 148:350–382

    Article  CAS  Google Scholar 

  • Loreto F, Velikova V (2001) Isoprene produced by leaves protects the photosynthetic apparatus against ozone damage, quenches ozone products, and reduces lipid peroxidation of cellular membranes. Plant Physiol 127:781–787

    Article  Google Scholar 

  • Martins N, Gonçalves S, Palma T, Romano A (2011) The influence of low pH on in vitro growth and biochemical parameters of Plantago almogravensis and P. algarbiensis. Plant Cell Tissue Organ Cult 107:113–121

  • Martins N, Gonçalves S, Andrade P, Valentão P, Romano A (2013a) Changes on organic acid secretion and accumulation in Plantago almogravensis Franco and P. algarbiensis Samp under aluminum stress. Plant Sci 198:1–6

    Article  CAS  PubMed  Google Scholar 

  • Martins N, Gonçalves S, Romano A (2013b) Metabolism and aluminum accumulation in Plantago almogravensis and P. algarbiensis in response to low pH and aluminum stress. Biol Plant 57:325–331

    Article  CAS  Google Scholar 

  • Martins N, Osório ML, Gonçalves S, Osório J, Palma T, Romano A (2013c) Physiological responses of Plantago algarbiensis and P. almogravensis shoots and plantlets to low pH and aluminum stress. Acta Physiol Plant 35:615–625

    Article  CAS  Google Scholar 

  • Martins N, Osório ML, Gonçalves S, Osório J, Romano A (2013d) Differences in Al tolerance between Plantago algarbiensis and P. almogravensis reflect their ability to respond to oxidative stress. Biometals 26:427–437

    Article  CAS  PubMed  Google Scholar 

  • Ncube B, Baskaran P, Van Staden J (2015) Transition from in vitro to an ex vitro environment: is the metabolism altered? In Vitro Cell Dev Biol—Plant 51:166–173

    Article  CAS  Google Scholar 

  • Obert B, Benson EE, Millam S, Preťová A, Bremner DH (2005) Moderation of morphogenetic and oxidative stress responses in flax in vitro cultures by hydroxynonenal and desferrioxamine. J Plant Physiol 162:537–547

    Article  CAS  PubMed  Google Scholar 

  • Osório ML, Gonçalves S, Osório J, Romano A (2005) Effects of CO2 concentration on acclimatization and physiological responses of two cultivars of carob tree. Biol Plant 49:161–167

    Article  Google Scholar 

  • Osório ML, Osório J, Romano A (2010) Chlorophyll fluorescence in micropropagated Rhododendron ponticum subsp. baeticum plants in response to different irradiances. Biol Plant 54:415–422

  • Osório ML, Osório J, Gonçalves S, David MM, Correia MJ, Romano A (2012) Carob trees (Ceratonia siliqua L.) regenerated in vitro can acclimatize successfully to match the field performance of seed-derived plants. Trees 26:1837–1846

  • Osório ML, Gonçalves S, Coelho N, Osório J, Romano A (2013) Morphological, physiological and oxidative stress markers during acclimatization and field transfer of micropropagated Tuberaria major plants. Plant Cell Tissue Organ Cult 115:85–97

    Article  Google Scholar 

  • Pospíšilová J, Tichá I, Kadleček P, Haisel D, Plzáková Š (1999) Acclimatization of micropropagated plants to ex vitro conditions. Biol Plant 42:481–497

    Article  Google Scholar 

  • Serrano HC, Pinto MJ, Martins-Loução MA, Branquinho C (2011) How does an Al-hyperaccumulator plant respond to a natural field gradient of soil phytoavailable Al? Sci Tot Environ 409:3749–3756

    Article  CAS  Google Scholar 

  • Singh PK, Tewari RK (2003) Cadmium toxicity induced changes in plant water relations and oxidative metabolism of Brassica juncea L. plants. J Environ Biol 24:107–112

    CAS  PubMed  Google Scholar 

Download references

Acknowledgments

S. Gonçalves acknowledges a grant from the Foundation for Science and Technology (FCT), Portugal (SFRH/BPD/84112/2012) financed by POPH-QREN and subsidized by the European Science Foundation.

Author information

Authors and Affiliations

  1. Faculty of Sciences and Technology, MeditBio, University of Algarve, Campus de Gambelas, Ed. 8, 8005-139, Faro, Portugal

    Sandra Gonçalves, Neusa Martins & Anabela Romano

Authors
  1. Sandra Gonçalves
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Neusa Martins
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Anabela Romano
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Anabela Romano.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Gonçalves, S., Martins, N. & Romano, A. Physiological traits and oxidative stress markers during acclimatization of micropropagated plants from two endangered Plantago species: P. algarbiensis Samp. and P. almogravensis Franco. In Vitro Cell.Dev.Biol.-Plant 53, 249–255 (2017). https://doi.org/10.1007/s11627-017-9812-y

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11627-017-9812-y

Keywords

Search

Navigation