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Metabolism and aluminum accumulation in Plantago almogravensis and P. algarbiensis in response to low pH and aluminum stress

  • Original Papers
  • Published:
Biologia Plantarum

Abstract

We investigated the impact of low pH and aluminum on the metabolism and capacity for Al accumulation in shoots of the plantain species Plantago algarbiensis and P. almogravensis. We found that increasing the concentration of Al in the medium increased accumulation of it in the shoots of both plants (although more in P. almogravensis than in P. algarbiensis). The presence of Al in the medium induced proline and saccharide synthesis in P. almogravensis without affecting lipid peroxidation, but increased proline synthesis and lipid peroxidation in P. algarbiensis without affecting the saccharide content. Lipid peroxidation in P. algarbiensis was also enhanced at pH 4.0. The activity of antioxidant enzymes was increased as a response to low pH and Al in both species. Our data indicate that both species can accumulate high levels of Al but they have different sensitivities to low pH and/or the presence of Al in the growth medium.

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Abbreviations

APX:

ascorbate peroxidase

CAT:

catalase

d.m.:

dry mass

f.m.:

fresh mass

GPX:

guaiacol peroxidase

MDA:

malondialdehyde

MS:

Murashige and Skoog medium

NBT:

nitroblue tetrazolium

SOD:

superoxide dismutase

TBA:

thiobarbituric acid

TCA:

trichloroacetic acid

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Authors and Affiliations

  1. Institute for Biotechnology and Bioengineering, Centre of Genomics and Biotechnology (IBB/CGB), Faculty of Sciences and Technology, University of Algarve, Campus de Gambelas, 8005-139, Faro, Portugal

    N. Martins, S. Gonçalves & A. Romano

Authors
  1. N. Martins
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  2. S. Gonçalves
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  3. A. Romano
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Correspondence to A. Romano.

Additional information

Acknowledgements: N. Martins and S. Gonçalves acknowledge grants SFRH/BD/48379/2008 and SFRH/BPD/31534/2006 from the Portuguese Science and Technology Foundation (FCT). This work was supported by the FCT project PTDC/AGRAAM/102664/2008. The authors acknowledge MC Costa for providing access to the Hach LANGE DR 2800 spectrophotometer. The first two authors equally contributed to this paper.

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Martins, N., Gonçalves, S. & Romano, A. Metabolism and aluminum accumulation in Plantago almogravensis and P. algarbiensis in response to low pH and aluminum stress. Biol Plant 57, 325–331 (2013). https://doi.org/10.1007/s10535-012-0271-3

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  • DOI: https://doi.org/10.1007/s10535-012-0271-3

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