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Enhancement of in vitro shoot regeneration from leaf explants of apple rootstock G.41

  • Plant Tissue Culture
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Abstract

Apple (Malus domestica) rootstock G.41 is an excellent member of the Geneva series because it has traits for resistance to abiotic and biotic stresses. A simple and efficient protocol for obtaining shoots from leaf explants was established by optimizing the combinations of plant growth regulators, mode of wounding, and explant orientation on the culture medium. The best shoot multiplication index (2.58) was obtained from successful subculture medium that was the standard Murashige and Skoog (MS) medium supplemented with 7.5 g L−1 agar, 3.55 μM N 6-benzyladenine, 0.16 μM indole-3-butyric acid, and 30 g L−1 sucrose. Regeneration rates were highest (99%) when MS medium was supplemented with 2.7 μM thidiazuron and 0.9 μM 1-naphthaleneacetic acid, and cut-wounding explants before placing the abaxial surface in contact with the medium. The best rooting percentage (80%) was obtained on MS medium supplemented with 4.92 μM indole-3-butyric acid. Plantlets were rooted in vitro and survived acclimatization in the laboratory and greenhouse.

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Acknowledgments

This work was supported by the National High Technology Research and Development Program of China (863 Program) (2011AA100201), and by the earmarked fund for the China Agriculture Research System (CARS-28). The authors are grateful to Priscilla Licht for help in revising our English composition.

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Authors and Affiliations

  1. State Key Laboratory of Crop Stress Biology for Arid Areas, College of Horticulture, Northwest A&F University, Yangling, Shaanxi, 712100, People’s Republic of China

    Xin Zhang, Yuan Qin, Dong Liang, Yangjun Zou & Fengwang Ma

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  1. Xin Zhang
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  2. Yuan Qin
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  3. Dong Liang
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  4. Yangjun Zou
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  5. Fengwang Ma
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Correspondence to Fengwang Ma.

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Editor: J. Forster

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Zhang, X., Qin, Y., Liang, D. et al. Enhancement of in vitro shoot regeneration from leaf explants of apple rootstock G.41. In Vitro Cell.Dev.Biol.-Plant 50, 263–270 (2014). https://doi.org/10.1007/s11627-013-9588-7

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  • DOI: https://doi.org/10.1007/s11627-013-9588-7

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