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The effect of in vitro pretreatments on subsequent shoot organogenesis from excised Rubus and Malus leaves

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Abstract

Shoot regeneration from Rubus leaves was obtained on a medium containing MS salts, vitamins and sugars, Staba vitamins, casein hydrolysate (100 mg l−1) and 10 μM thidiazuron. Shoot regeneration from Malus leaves was obtained on N6 rice anther medium with 5 μM thidiazuron. In vitro pretreatment of source shoots with either colchicine or thidiazuron enhanced the organogenic potential of detached leaves of two Rubus hybrids. The response to colchicine was quadratic and occurred at non-mutagenic concentrations (75–250 μM). The response to thidiazuron was exponential between 0 and 5 μM. When applied as a pretreatment, the effectiveness of several different cytokinins (benzyladenine, thidiazuron, zeatin) at enhancing Malus and Rubus organogenesis was related to the shoot proliferation activity of the cytokinin and to treatment-induced variation in leaf and petiole size.

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Abbreviations

BA:

benzyladenine

2,4-D:

2,4-dichlorophenoxyacetic acid

IBA:

indolebutyric acid

MS:

Murashige & Skoog basal medium devoid of plant growth regulators

OI:

organogenesis-initiating subculture

PTI:

colchicine pretreatment subculture

PTII:

cytokinin pretreatment subculture

NAA:

naphthaleneacetic acid

TDZ:

thidiazuron

zeatin:

trans-zeatin

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Authors and Affiliations

  1. Department of Horticulture, University of Maryland, 20742-5611, College Park, MD, USA

    Harry Jan Swartz, Robert Bors, Fouad Mohamed & S. Kristine Naess

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  1. Harry Jan Swartz
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  2. Robert Bors
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  3. Fouad Mohamed
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  4. S. Kristine Naess
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Swartz, H.J., Bors, R., Mohamed, F. et al. The effect of in vitro pretreatments on subsequent shoot organogenesis from excised Rubus and Malus leaves. Plant Cell Tiss Organ Cult 21, 179–184 (1990). https://doi.org/10.1007/BF00033439

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  • DOI: https://doi.org/10.1007/BF00033439

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