Summary
Apple (Malus domestica) transgenic T1 was obtained by Agrobacterium tumefaciens- mediated transformation of Mailing 26 rootstock using the plasmid binary vector pLDB 15. pLDB 15 contains within its T-DNA a gene encoding the lytic protein attacin E. The integration of the attacin E gene into the apple genome was confirmed by Southern analysis. Northern analysis indicated the presence of an attacin E mRNA in plants inoculated with Erwinia amylovora. After inoculation of in vitro grown plants of T1, Mailing 26, and Mailing 7 (resistant control) with E. amylovora, the log10 of the inoculum concentration lethal to 50% of the plants was 5.4, 4.4, and 5.6, respectively. In greenhouse trials for resistance to fire blight, T1 was significantly more resistant than‘Mailing 26’.
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Abbreviations
- DNA:
-
deoxyribonucleic acid
- GUS:
-
beta-glucuronidase
- M.7:
-
MaIling 7
- M.26:
-
MaIling 26
- MU:
-
4-rnethylumbelliferone
- MUG:
-
4-methylumbelliferyl-beta-D-glucuronide
- nptII:
-
neomycin phosphotransferase gene
- RNA:
-
ribonucleic acid
- X-gluc:
-
5-Bromo-4-chloro-3-indolyl-beta-D-glucuronic acid
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© 1994 Springer Science+Business Media Dordrecht
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Norelli, J.L., Aldwinckle, H.S., Destéfano-Beltrán, L., Jaynes, J.M. (1994). Transgenic ‘Malling 26’ apple expressing the attacin E gene has increased resistance to Erwinia amylovora . In: Schmidt, H., Kellerhals, M. (eds) Progress in Temperate Fruit Breeding. Developments in Plant Breeding, vol 1. Springer, Dordrecht. https://doi.org/10.1007/978-94-011-0467-8_67
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