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Mesos components (CaCl2, MgSO4, KH2PO4) are critical for improving pear micropropagation

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Abstract

Pear accessions and species show a broad response to tissue culture media due to the wide genetic diversity that exists in the available pear germplasm. An initial study of mineral nutrition using a systematic response surface approach with five Murashige and Skoog medium mineral stock solutions indicated that the mesos factor (CaCl2, MgSO4, and KH2PO4) affected most plant responses and genotypes, suggesting that additional studies were needed to further optimize these three mesos components for a wide range of genotypes. Short stature, leaf spots, edge necrosis, and red or yellow coloration were the main symptoms of poor nutrition in shoot cultures of 10 diverse pear genotypes from six species. A surface response experimental design was used to model the optimal factor and factor levels for responses that included overall quality, leaf character, shoot multiplication, and shoot height. The growth morphology, shoot length, and multiplication of these pear shoots could be manipulated by adjusting the mesos components. The highest quality for the majority of genotypes, including five P. communis cultivars, P. koehnei, P. dimorphophylla, and P. pyrifolia ‘Sion Szu Mi’, required higher concentrations (>1.2× to 2.5×) of all the components than are present in Murashige and Skoog medium. ‘Capital’ (P. calleryana) required high CaCl2 and MgSO4 with low KH2PO4; for ‘Hang Pa Li’ (P. ussuriensis), low CaCl2 and moderate to low MgSO4 and KH2PO4 produced high-quality shoots. Suitable combinations of the meso nutrients produced both optimum shoot number and shoot length in addition to general good plant quality.

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Acknowledgments

We thank the NCGR lab personnel for assistance with collection of the data. This project was funded by a grant from the Oregon Association of Nurseries and the Oregon Department of Agriculture and by United States Department of Agriculture, Agricultural Research Service CRIS project 5358-21000-0-38-00D.

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Authors and Affiliations

  1. Department of Horticulture, Oregon State University, 4017 Agriculture & Life Science Bldg, Corvallis, OR, 97331-7304, USA

    Sugae Wada

  2. Department of Agriculture, Agricultural Research Service, U.S. Horticultural Research Laboratory, 2001 South Rock Road, Ft. Pierce, FL, 34945-3030, USA

    Randall P. Niedz

  3. Department of Agriculture, Agricultural Research Service, National Clonal Germplasm Repository, 33447 Peoria Rd, Corvallis, OR, 97333-2521, USA

    Jeanine DeNoma & Barbara M. Reed

Authors
  1. Sugae Wada
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  2. Randall P. Niedz
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  3. Jeanine DeNoma
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  4. Barbara M. Reed
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Correspondence to Sugae Wada.

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Editor: Dave Duncan

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Wada, S., Niedz, R.P., DeNoma, J. et al. Mesos components (CaCl2, MgSO4, KH2PO4) are critical for improving pear micropropagation. In Vitro Cell.Dev.Biol.-Plant 49, 356–365 (2013). https://doi.org/10.1007/s11627-013-9508-x

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