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Current and future editing reagent delivery systems for plant genome editing

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Abstract

Many genome editing tools have been developed and new ones are anticipated; some have been extensively applied in plant genetics, biotechnology and breeding, especially the CRISPR/Cas9 system. These technologies have opened up a new era for crop improvement due to their precise editing of user-specified sequences related to agronomic traits. In this review, we will focus on an update of recent developments in the methodologies of editing reagent delivery, and consider the pros and cons of current delivery systems. Finally, we will reflect on possible future directions.

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Authors and Affiliations

  1. Genovo Biotechnology Co., Ltd., Tianjin, 301700, China

    Yidong Ran

  2. State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, 100101, China

    Zhen Liang & Caixia Gao

  3. University of Chinese Academy of Sciences, Beijing, 100049, China

    Zhen Liang

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  1. Yidong Ran
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Correspondence to Yidong Ran or Caixia Gao.

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Ran, Y., Liang, Z. & Gao, C. Current and future editing reagent delivery systems for plant genome editing. Sci. China Life Sci. 60, 490–505 (2017). https://doi.org/10.1007/s11427-017-9022-1

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