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Analysis of transmission of heterozygosity by 2n gametes in Populus (Salicaceae)

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Abstract

It is well known that polyploidy is a major force in plant evolution and diversification. Evaluating the transmission of heterozygosity by 2n gametes with different formation mechanisms is of crucial importance. In the present study, 120 triploid hybrids [originating from first-division restitution (FDR), second-division restitution (SDR), and postmeiotic restitution (PMR) 2n eggs of the same female parent] and 30 pairs of polymorphic simple sequence repeat (SSR) markers (distributed across all 19 chromosomes of Populus) were used to evaluate the maternal heterozygosity transmitted by each type of 2n eggs. FDR, SDR, and PMR 2n eggs transmitted different parental heterozygosities (0.7480, 0.3958, and 0.3590, respectively) in Populus. In addition, compared with the small coefficient of variation (CV = 13.50 %) of heterozygosity transmitted by the 19 chromosomes of FDR 2n eggs, there was greater variation in heterozygosity transmitted by SDR and PMR 2n eggs (CV = 51.92 % and CV = 63.14 %, respectively). This may have resulted from the locations of the applied SSR markers, in addition to the different formation mechanisms of 2n gametes. Variations in locus-centromere distance resulted in variations in recombination rates, which influence the degree of heterozygosis at the detected locus. In conclusion, our data provides a useful reference point for the suitable applications of 2n gametes in future studies, promoting the further research of polyploidy in plants.

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Acknowledgments

This work was financially supported by the Special Fund for Forest Scientific Research in the Public Welfare (201404113), the National Natural Science Foundation of China (31470667), and the 111 Project (B13007). We would like to acknowledge members of our labs for advice and discussions. We thank the Forestry Research Institute of Tongliao City, the Inner Mongolia Autonomous Region, People’s Republic of China, for collecting the plant material and for additional help.

Data archiving statement

SSR markers used in this study are from the International Populus Genome Consortium (IPGC, http://www.ornl.gov/sci/ipgc/ssr_resource.htm). Information of all the applied SSR markers are available as supplemental text files that can be downloaded with the manuscript.

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Authors and Affiliations

  1. National Engineering Laboratory for Tree Breeding, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, People’s Republic of China

    Chun-Bo Dong, Yu-Jing Suo, Jun Wang & Xiang-Yang Kang

  2. Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, People’s Republic of China

    Chun-Bo Dong, Yu-Jing Suo, Jun Wang & Xiang-Yang Kang

Authors
  1. Chun-Bo Dong
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  2. Yu-Jing Suo
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  3. Jun Wang
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  4. Xiang-Yang Kang
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Correspondence to Xiang-Yang Kang.

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Communicated by A. Brunner

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Dong, CB., Suo, YJ., Wang, J. et al. Analysis of transmission of heterozygosity by 2n gametes in Populus (Salicaceae). Tree Genetics & Genomes 11, 799 (2015). https://doi.org/10.1007/s11295-014-0799-9

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  • DOI: https://doi.org/10.1007/s11295-014-0799-9

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