Skip to main content
SpringerLink
Log in

A cherry map from the inter-specific cross Prunus avium ‘Napoleon’ × P. nipponica based on microsatellite, gene-specific and isoenzyme markers

  • Original Paper
  • Published:
Tree Genetics & Genomes Aims and scope Submit manuscript

Abstract

One hundred and sixty microsatellite (simple sequence repeat (SSR)) and six gene-specific markers revealing 174 loci were scored in 94 seedlings from the inter-specific cross of Prunus avium ‘Napoleon’ × Prunus nipponica accession F1292. The co-segregation data from these markers were used to construct a linkage map for cherry which spanned 680 cM over eight linkage groups with an average marker spacing of 3.9 cM per marker and just six gaps longer than 15 cM. Markers previously mapped in Prunus dulcis ‘Texas’ × Prunus persica ‘Earlygold’ allowed the cherry map to be anchored to the peach × almond map and showed the high level of synteny between the species. Eighty-four loci segregated in P. avium ‘Napoleon’ versus 159 in P. nipponica. The segregations of 32 isoenzyme loci in a subset of 47 seedlings from the progeny were scored, using polyacrylamide gel electrophoresis and/or isoelectric focusing separation followed by activity staining, and the co-segregation data were analysed along with those for 39 isoenzymes reported previously and for the 174 sequence-tagged site loci plus an additional two SSR loci. The second map incorporates 233 loci and spans 736 cM over eight linkage groups with an average marker spacing of 3.2 cM per marker and just two gaps greater than 15 cM. The microsatellite map will provide a useful tool for cherry breeding and marker-assisted selection and for synteny studies within Prunus; the gene-specific markers and isoenzymes will be useful for comparisons with maps of other rosaceous fruit crops.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2

Similar content being viewed by others

References

  • Aranzana MJ, Garcia-Mas J, Carbo J, Arus P (2002) Development and variability analysis of microsatellite markers in peach. Plant Breed 121:87–92

    Article  CAS  Google Scholar 

  • Aranzana MJ, Pineda A, Cosson P, Dirlewanger E, Ascasibar J, Cipriani G, Ryder CD, Testolin R, Abbott A, King GJ, Iezzoni AF, Arus P (2003) A set of simple-sequence repeat (SSR) markers covering the Prunus genome. Theor Appl Genet 106:819–825

    PubMed  CAS  Google Scholar 

  • Ballester J, Boskovic R, Batlle I, Arus P, Vargas F, de Vicente MC (1998) Location of the self-incompatibility gene on the almond linkage map. Plant Breed 117:69–72

    Article  Google Scholar 

  • Blenda AV, Verde I, Georgi LL, Reighard GL, Forrest SD, Muñoz-Torres M, Baird WV, Abbott AG (2007) Construction of a genetic linkage map and identification of molecular markers in peach rootstocks for response to peach tree short life syndrome. Tree Genet Gen 3:341–350

    Article  Google Scholar 

  • Bošković R, Tobutt KR (1996) Correlation of stylar ribonuclease zymograms with incompatibility alleles in sweet cherry. Euphytica 90:245–250

    Article  Google Scholar 

  • Bošković R, Tobutt KR (1998) Inheritance and linkage relationships of isoenzymes in two interspecific cherry progenies. Euphytica 103:273–286

    Article  Google Scholar 

  • Bošković R, Tobutt KR, Arus P, Messeguer R (1994) Isoenzymes. In: Messeguer R (ed) Methods of molecular marker analysis in Prunus. IRTA, Barcelona, pp 4–25

    Google Scholar 

  • Bošković R, Tobutt KR, Nicoll FJ (1997) Inheritance of isoenzymes and their linkage relationships in two interspecific cherry progenies. Euphytica 93:129–143

    Article  Google Scholar 

  • Cantini C, Iezzoni AF, Lamboy WF, Boritzki M, Struss D (2001) DNA fingerprinting of tetraploid cherry germplasm using simple sequence repeats. J Amer Soc Hortic Sci 126:205–209

    CAS  Google Scholar 

  • Cheliak WM, Pitel JA (1984) Techniques for starch gel electrophoresis of enzymes from forest tree species. In: Information report PI-X-42 of Petawawa National Forestry Institute. Agriculture Canada, pp 1–49

  • Chevreau E, Leuliette S, Gallet M (1997) Inheritance and linkage of isoenzyme loci in pear (Pyrus communis L.). Theor Appl Genet 94:498–506

    Article  CAS  Google Scholar 

  • Cipriani G, Lot G, Huang WG, Marrazzo MT, Peterlunger E, Testolin R (1999) AC/GT and AG/CT microsatellite repeats in peach (Prunus persica (L.) Batsch): isolation, characterization and cross-species amplification in Prunus. Theor Appl Genet 99:65–72

    Article  CAS  Google Scholar 

  • Clarke JB, Tobutt KR (2003) Development and characterisation of polymorphic microsatellites Prunus avium ‘Napoleon’. Mol Ecol Notes 3:578–580

    Article  CAS  Google Scholar 

  • Davis TM, Yu H (1997) A linkage map of the diploid strawberry, Fragaria vesca. J Hered 88:215–221

    CAS  Google Scholar 

  • Dirlewanger E, Cosson P, Tavaud M, Aranzana MJ, Poizat C, Zanetto A, Arus P, Laigret F (2002) Development of microsatellite markers in peach (Prunus persica (L.) Batsch) and their use in genetic diversity analysis in peach and sweet cherry (Prunus avium L.). Theor Appl Genet 105:127–138

    Article  PubMed  CAS  Google Scholar 

  • Dirlewanger E, Graziano E, Joobeur T, Garriga-Calderé F, Cosson P, Howad W, Arús P (2004) Comparative mapping and marker-assisted selection in Rosaceae fruit crops. PNAS 101:9891–9896

    Article  PubMed  CAS  Google Scholar 

  • Dondini L, Costa E, Tataranni G, Tartarini S, Sansavini S (2006) Cloning of apricot RGAs (resistance gene analogs) and development of molecular markers associated with Sharka (PPV) resistance. J Hort Sci Biotech 79:729–734

    Google Scholar 

  • Downey SL, Iezzoni AF (2000) Polymorphic DNA markers in black cherry (Prunus serotina) are identified using sequences from sweet cherry, peach, and sour cherry. J Amer Soc Hort Sci 125:76–80

    CAS  Google Scholar 

  • Etienne C, Rothan C, Moing A, Plomion C, Bodénès C, Svanella-Dumas L, Cosson P, Pronier V, Monet R, Dirlewanger E (2002) Candidate genes and QTLs for sugar and organic acid content in peach [Prunus persica (L.) Batsch]. Theor Appl Genet 105:145–159

    Article  PubMed  CAS  Google Scholar 

  • Fishman L, Kelly AJ, Morgan E, Willis JH (2001) A genetic map in the Mimulus guttatus species complex reveals transmission ratio distortion due to heterospecific interactions. Genetics 159:1701–1716

    PubMed  CAS  Google Scholar 

  • Foulongne M, Pascal T, Arus P, Kervella J (2003) The potential of Prunus davidiana for introgression into peach [Prunus persica (L.) Batsch] assessed by comparative mapping. Theor Appl Genet 107:227–238

    Article  PubMed  CAS  Google Scholar 

  • Fukatsu E, Isoda K, Hirao T, Takahashi M, Watanabe A (2005) Development and characterization of simple sequence repeat DNA markers for Zelkova serrata. Mol Ecol Notes 5:378–380

    Article  CAS  Google Scholar 

  • Hagen LS, Chaib J, Fady B, Decroocq V, bouchet JP, Lambert P, Audergon JM (2004) Genomic and cDNA microsatellites from apricot (Prunus armeniaca L.). Mol Ecol Notes 4:742–745

    Article  CAS  Google Scholar 

  • Hemmat M, Weeden NF, Manganaris AG, Lawson DM (1994) Molecular marker linkage map of apple. Heredity 85:4–11

    CAS  Google Scholar 

  • Howad W, Yamamoto T, Dirlewanger E, Testolin R, Cosson P, Cipriani G, Monforte A, Georgi L, Abbott A, Arus P (2005) Mapping with a few plants: using selective mapping for microsatellite saturation of the Prunus reference map (2005). Genetics 171:1305–1309

    Article  PubMed  CAS  Google Scholar 

  • Jaaska V (1978) NADP-dependent aromatic alcohol dehydrogenase in polyploid wheats and their diploid relatives. On the origin and phylogeny of polyploid wheats. Theor Appl Genet 53:209–217

    Article  CAS  Google Scholar 

  • James CM, Wilson F, Hadonou AM, Tobutt KR (2003) Isolation and characterization of polymorphic microsatellites in diploid strawberry (Fragaria vesca L.) for mapping, diversity studies and clone identification. Mol Ecol Notes 3:171–173

    Article  CAS  Google Scholar 

  • Joobeur T, Viruel MA, de Vicente MC, Jauregui B, Ballester J, Dettori MT, Verde I, Truco MJ, Messeguer R, Battle I, Quarta R, Dirlewanger E, Arus P (1998) Construction of a saturated linkage map for Prunus using an almond × peach F2 progeny. Theor Appl Genet 97:1034–1041

    Article  CAS  Google Scholar 

  • Joobeur T, Periam N, Vicente MC, King GJ, Arus P (2000) Development of a second generation linkage map for almond using RAPD and SSR markers. Genome 43:649–655

    Article  PubMed  CAS  Google Scholar 

  • Kobayashi N, Horikoshi T, Katsuyama H, Handa T, Takayanagi K (1998) A simple and efficient DNA extraction method for plants, especially woody plants. Plant Cell Tiss Org Cult 4:76–80

    Google Scholar 

  • Lee S, Wen J (2001) A phylogenetic analysis of Prunus and the Amygdaloideae (Rosaceae) using ITS sequences of nuclear ribosomal DNA. Amer J Botany 88:150–160

    Article  CAS  Google Scholar 

  • Lopes MS, Sefc KM, Laimer M, Da Camaro Machado A (2002) Identification of microsatellite loci in apricot. Mol Ecol Notes 2:24–26

    Article  CAS  Google Scholar 

  • Messina R, Lain O, Marrazzo T, Cipriani G, Testolin R (2004) New set of microsatellite loci isolated in apricot. Mol Ecol Notes 4:432–434

    Article  CAS  Google Scholar 

  • Mnejja M, Garcia-Mas J, Howad W, Badenes ML, Arus P (2004) Simple-sequence repeat (SSR) markers of Japanese plum (Prunus salicina Lindl.) are highly polymorphic and transferable to peach and almond. Mol Ecol Notes 4:163–166

    Article  CAS  Google Scholar 

  • Mnejja M, Garcia-Mas J, Howad W, Arus P (2005) Development and transportability across Prunus species of 42 polymorphic almond microsatellites. Mol Ecol Notes 5:531–535

    Article  CAS  Google Scholar 

  • Pashkoulov DT, Tobutt KR, Bošković R (2000) Comparison of isoenzymes in Prunus avium separated by two different electrophoretic techniques. Plant Breeding 119:153–156

    Article  CAS  Google Scholar 

  • Santi F, Lemoine M (1990) Genetic markers for Prunus avium L.: inheritance and linkage of isoenzyme loci. Ann Forest Sci 47:131–139

    Article  Google Scholar 

  • Sargent DJ, Rys A, Nier S, Simpson DW, Tobutt KR (2007) The development and mapping of functional markers in Fragaria and their transferability and potential for mapping in other genera. Theor Appl Genet 114:373–384

    Article  PubMed  CAS  Google Scholar 

  • Schuster M, Tobutt KR (2004) Screening of cherries for resistance to leaf spot, Blumeriella jaapii. Acta Hort 663:239–243

    Google Scholar 

  • Sonneveld T, Robbins TP, Tobutt KR (2006) Improved discrimination of self-incompatibility S-RNase alleles in cherry and high throughput genotyping by automated sizing of first intron polymerase chain reaction products. Plant Breed 125:305–307

    Article  CAS  Google Scholar 

  • Sosinski B, Gannavarapu M, Hager LD, Beck LE, King GJ, Ryder CD, Rajapakse S, Baird WV, Ballard RE, Abbott AG (2000) Characterization of microsatellite markers in peach (Prunus persica (L.) Batsch). Theor Appl Genet 101:421–428

    Article  CAS  Google Scholar 

  • Struss D, Boritzki M, Karle R, Iezzoni AF (2002) Microsatellite markers differentiate eight Giessen cherry rootstocks. HortScience 37:191–193

    CAS  Google Scholar 

  • Struss D, Ahmad R, Southwick SM (2003) Analysis of sweet cherry (Prunus avium L.) cultivars using SSR and AFLP markers. J Amer Soc Hort Sci 128:904–909

    CAS  Google Scholar 

  • Testolin R, Marrazzo T, Cipriani G, Quarta R, Verde I, Dettori MT, Pancaldi M, Sansavini S (2000) Microsatellite DNA in peach (Prunus persica L. Batsch) and its use in fingerprinting and testing the genetic origin of cultivars. Genome 43:512–520

    Article  PubMed  CAS  Google Scholar 

  • Testolin R, Messina R, Lain O, Marrazzo MT, Huang W-G, Cipriani G (2004) Microsatellites isolated in almond from an AC-repeat enriched library. Mol Ecol Notes 4:459–461

    Article  CAS  Google Scholar 

  • Tobutt KR (1985) New approaches to breeding sweet cherry scion varieties at East Malling with particular reference to small tree size. Acta Hort 160:43–50

    Google Scholar 

  • Van Ooijen JW, Voorrips RE (2001) JoinMap 3.0: software for the calculation of genetic linkage maps. Plant Research International, Wageningen

    Google Scholar 

  • Vaughan SP, Russell K (2004) Characterization of novel microsatellites and development of multiplex PCR for large-scale population studies in wild cherry, Prunus avium. Mol Ecol Notes 4:429–431

    Article  CAS  Google Scholar 

  • Verde I, Lauria M, Dettori M, Vendramin E, Balconi C, Micali S, Wang Y, Marrazzo MT, Cipriani G, Hartings H, Testolin R, Abbott AG, Motto M, Quarta R (2005) Microsatellite and AFLP markers in the Prunus persica [L. (Batsch)] x P. ferganensis BC1 linkage map: saturation and coverage improvement. Theor Appl Genet 111:1013–1021

    Article  PubMed  CAS  Google Scholar 

  • Viruel MA, Messeguer R, de Vicente MC, Garcia-Mas J, Puigdomènech P, Vargas F, Arus P (1995) A linkage map with RFLP and isoenzyme markers for almond. Theor Appl Genet 91:964–971

    Article  CAS  Google Scholar 

  • Voorrips RE (2002) MapChart: software for the graphical presentation of linkage maps and QTLs. J Hered 93:77–78

    Article  PubMed  CAS  Google Scholar 

  • Wendel JF, Weeden NF (1989) Visualisation and interpretation of plant isozymes. In: Soltis DE, Soltis PS (eds) Isozymes in plant biology. Dioscorides, Oregon, pp 5–45

    Google Scholar 

  • Yamamoto T, Mochida K, Imai T, Shi YZ, Ogiwara I, Hayashi T (2002) Microsatellite markers in peach [Prunus persica (L.) Batsch] derived from an enriched genomic and cDNA libraries. Mol Ecol Notes 2:298–301

    Article  CAS  Google Scholar 

  • Yamamoto T, Yamaguchi M, Hayashi T (2005) An integrated genetic linkage map of peach by SSR, STS, AFLP and RAPD. J Japan Soc Hort Sci 74:204–213

    Article  CAS  Google Scholar 

Download references

Acknowledgements

Stone fruit genetics at East Malling Research is funded by Defra. A. Belaj acknowledges a grant from Instituto Andaluz de Investigación y Formación Agraria y Pesquera, Conserjería de Innovación, Ciencia y Empresa, Andalucía, Spain and R. Bošković a grant from the Mount Trust.

Author information

Authors and Affiliations

  1. East Malling Research (EMR), New Road, East Malling, Kent, ME19 6BJ, UK

    J. B. Clarke, D. J. Sargent, R. I. Bošković, A. Belaj & K. R. Tobutt

  2. Imperial College at Wye, Ashford, Kent, TN25 5AH, UK

    R. I. Bošković

  3. Centro “Alameda del Obispo”, IFAPA, Avda. Menéndez Pídal s/n Apdo. 3092, 14071, Córdoba, Spain

    A. Belaj

Authors
  1. J. B. Clarke
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. D. J. Sargent
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. I. Bošković
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Belaj
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. K. R. Tobutt
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to D. J. Sargent.

Additional information

Communicated by M. Morgante.

Electronic supplementary material

Below is the link to the electronic supplementary material

Table S1

Monogenic segregation ratios and chi-squared values for goodness of fit to expected Mendelian segregation ratios for 174 loci (160 SSR and six gene-specific markers) scored in PA × PN including their linkage group location. The suffixes A, B and C distinguish multiple loci revealed by particular primers. Segregation ratios deviating significantly from the expected ratios (P ≤ 0.05, 0.01, 0.001) are indicated with one, two or three asterisks, respectively (DOC 264 KB)

Table S2

Monogenic segregation ratios and chi-squared values for goodness of fit to expected Mendelian segregation ratios for 32 isoenzyme and two SSR loci scored in a 47 seedling subset of the PA × PN progeny including their linkage group location. The suffixes -1, -2, -3, etc. distinguish multiple isoenzyme loci detected with PAGE and -a, -b, -c for cathodal and -z, -y, -x, etc. for anodal loading with IEF. Segregation ratios deviating significantly from the expected ratios (P ≤ 0.05, 0.01, 0.001) are indicated with one, two or three asterisks, respectively (DOC 79.0 KB)

Rights and permissions

Reprints and permissions

About this article

Cite this article

Clarke, J.B., Sargent, D.J., Bošković, R.I. et al. A cherry map from the inter-specific cross Prunus avium ‘Napoleon’ × P. nipponica based on microsatellite, gene-specific and isoenzyme markers. Tree Genetics & Genomes 5, 41–51 (2009). https://doi.org/10.1007/s11295-008-0166-9

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11295-008-0166-9

Keywords

Search

Navigation