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Development of leucine-rich repeat polymorphism, amplified fragment length polymorphism, and sequence characterized amplified region markers to the Cronartium ribicola resistance gene Cr2 in western white pine (Pinus monticola)

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Abstract

White pine blister rust (WPBR), caused by Cronartium ribicola, is a devastating disease in Pinus monticola and other five-needle pines. Pyramiding a major resistance gene (Cr2) with other resistance genes is an important component of integrated strategies to control WPBR in P. monticola. To facilitate this strategy, the objective of the present study was to identify leucine-rich repeat (LRR) polymorphisms, amplified fragment length polymorphisms (AFLPs), and sequence characterized amplified region (SCAR) markers linked to the western white pine Cr2 (BSA) gene for precise gene mapping. Bulked segregant analysis and haploid segregation analysis allowed the identification of 11 LRR polymorphisms and five AFLP markers in the Cr2 linkage. The closest LRR markers were 0.53 Kosambi cM from Cr2 at either end. After marker cloning and sequencing, AFLP marker EacccMccgat-365 and random polymorphic DNA marker U570–843 were converted successfully into SCAR markers. For a potential application in marker-assisted selection (MAS), these two SCAR markers were verified in two western white pine families. This study represents the first report of LRR-related DNA markers linked to C. ribicola resistance in five-needle pines. These findings may help further candidate gene identification for disease resistance in a conifer species.

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Acknowledgments

This research was supported by the forest genomics funding from the Canadian Forest Service of Natural Resources Canada. We thank Dr. J. Eades and Miss P. Rajkumar for technical assistance with AFLP gel electrophoresis and SCAR marker tests, and Dr. P. Dale, H. Williams, and J. Stone for thoughtful comments on the manuscript

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  1. Pacific Forestry Centre, Natural Resources Canada, 506 West Burnside Road, Victoria, British Columbia, V8Z 1M5, Canada

    Jun-Jun Liu & Abul K. M. Ekramoddoullah

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  1. Jun-Jun Liu
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  2. Abul K. M. Ekramoddoullah
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Correspondence to Abul K. M. Ekramoddoullah.

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Communicated by S. Aitken

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Liu, JJ., Ekramoddoullah, A.K.M. Development of leucine-rich repeat polymorphism, amplified fragment length polymorphism, and sequence characterized amplified region markers to the Cronartium ribicola resistance gene Cr2 in western white pine (Pinus monticola). Tree Genetics & Genomes 4, 601–610 (2008). https://doi.org/10.1007/s11295-008-0135-3

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  • DOI: https://doi.org/10.1007/s11295-008-0135-3

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