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Resistance to rust (Puccinia psidii Winter) in Eucalyptus: mode of inheritance and mapping of a major gene with RAPD markers

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Abstract

Rust is one of the most-damaging eucalypt diseases in Brazil and is considered a potential threat to eucalypt plantations worldwide. To determine the mode of inheritance of resistance in the Eucalyptus grandis—Puccinia psidii pathosystem, ten full-sib families, generated from crosses between susceptible and resistant trees, were inoculated with a single-pustule isolate of the pathogen and rust severity was scored. The observed segregation ratios in segregating families suggested major gene control of rust resistance, although clearly incomplete penetrance, variable expressivity and minor genes are also involved in the global rust-resistance response. To identify markers linked to the resistance locus, screening of RAPD polymorphisms was conducted using bulked segregant analysis in a large full-sib family. A linkage group was built around the Ppr1 gene (P. psidii resistance gene 1) encompassing six RAPD markers, with a genetic window spanning 5 cM with the two most-closely linked flanking markers. Besides these two flanking markers, RAPD marker AT9/917 co-segregated with Ppr1 without a single recombinant in 994 meioses. This tightly linked marker should prove useful for marker-assisted introgression and will provide an initial lead for a positional cloning effort of this resistance allele. This is the first report of a disease resistance gene identified in Eucalyptus, and one of the few examples of the involvement of a major gene in a non-coevolved pathosystem.

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Acknowledgments

We thank Inez Tommerup (CSIRO, Australia) for reviewing the script. This work was supported by Cia. Suzano de Papel e Celulose, FAPEMIG—Fundação de Amparo à Pesquisa de Minas Gerais (Fapemig) and CNPq—Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).

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Authors and Affiliations

  1. Departamento de Fitopatologia, Universidade Federal de Viçosa, 36571-000, Viçosa, MG, Brazil

    D. T. Junghans

  2. Embrapa Mandioca e Fruticultura, Caixa Postal 007, Cruz das Almas, BA, Brazil

    D. T. Junghans

  3. Departamento de Fitopatologia, Universidade Federal de Viçosa, 36571-000, Viçosa, MG, Brazil

    A. C. Alfenas & S. H. Brommonschenkel

  4. Cia. Suzano de Papel e Celulose - DRN, Prudente de Moraes, 4006, 08613-900, Suzano, SP, Brazil

    S. Oda & E. J. Mello

  5. Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, 70770-970, Brasilia, DF, Brazil

    D. Grattapaglia

  6. Graduate Program in Genomic Sciences and Biotechnology, Universidade Catolica de Brasília, SGAN 916 modulo B, 70790-160, Brasília, DF, Brazil

    D. Grattapaglia

Authors
  1. D. T. Junghans
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  2. A. C. Alfenas
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  3. S. H. Brommonschenkel
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  4. S. Oda
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  5. E. J. Mello
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  6. D. Grattapaglia
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Corresponding author

Correspondence to A. C. Alfenas.

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Communicated by D.B. Neale

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Junghans, D.T., Alfenas, A.C., Brommonschenkel, S.H. et al. Resistance to rust (Puccinia psidii Winter) in Eucalyptus: mode of inheritance and mapping of a major gene with RAPD markers. Theor Appl Genet 108, 175–180 (2003). https://doi.org/10.1007/s00122-003-1415-9

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  • DOI: https://doi.org/10.1007/s00122-003-1415-9

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