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Genome characteristics dictate poly-R-(3)-hydroxyalkanoate production in Cupriavidus necator H16

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Abstract

Cupriavidus necator H16 is a well-recognized enterprise with efficient manufacturing machineries to produce diverse polymers belonging to polyhydroxyalkanoates (PHAs) family. The genome fingerprints, including PHA machinery proteins and fatty acid metabolism, had educated engineering strategies to enhance PHAs production. This outstanding progress has enlightened us to present an exhaustive examination of the ongoing research, addressing the great potential design of genome features towards PHA production and furthermore, we show how those acquired knowledge have been explored in other biotechnological applications. This updated-review concludes that the combination of an optimal strain selection, suitable metabolic engineering and a large-scale fermentation on oil substrates is critical to endow the ability of incorporating mcl-PHAs monomers in this organism.

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Acknowledgements

The authors would like to recognize Dr. Madel Lopez Cuellar Rocio and Dr. Arthi Rathinasabapathy for their appreciative contributions in the investigations conducted in our laboratory. This work is financially supported by CONACyT (CB-2014-01; 236285 and Fronteras de la Ciencia 2015-1: 016). The authors thank Miguel Martinez Roque and Juan Corona-Hernandez for their artwork contribution.

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  1. Department of Biotechnology and Bioengineering, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, Av. IPN 2508, Zacatenco Gustavo A. Madero, México D.F., Mexico

    Gurusamy Kutralam-Muniasamy & Fermín Peréz-Guevara

  2. Nanoscience & Nanotechnology Program, Centro de Investigación y de Estudios Avanzados del Instituto Politécnico Nacional, México D.F., Mexico

    Fermín Peréz-Guevara

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  1. Gurusamy Kutralam-Muniasamy
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  2. Fermín Peréz-Guevara
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Correspondence to Fermín Peréz-Guevara.

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Kutralam-Muniasamy, G., Peréz-Guevara, F. Genome characteristics dictate poly-R-(3)-hydroxyalkanoate production in Cupriavidus necator H16. World J Microbiol Biotechnol 34, 79 (2018). https://doi.org/10.1007/s11274-018-2460-5

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