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Structural characterization of rhamnolipid produced by Pseudonomas aeruginosa strain FIN2 isolated from oil reservoir water

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Abstract

Biosurfactant-producing microorganisms inhabiting oil reservoirs are of great potential in industrial applications. Yet, till now, the knowledge about the structure and physicochemical property of their metabolites are still limited. The aim of this study was to purify and structurally characterize the biosurfactant from Pseudomonas aeruginosa strain FIN2, a newly isolated strain from an oil reservoir. The purification was conducted by silica gel column chromatography followed by pre-RP HPLC and the structural characterization was carried out by GC–MS combined with MS/MS. The results show that the biosurfactant produced by FIN2 is rhamnolipid in nature and its four main fractions were identified to be Rha-C10-C10(46.1 %), Rha–Rha-C10-C10(20.1 %), Rha-C8-C10 (7.5 %) and Rha-C10-C12:1(5.5 %), respectively. Meanwhile, the rarely reported rhamnolipid congeners containing β-hydroxy fatty acids of C6, C9, C10:1 and C11 were also proved to be present in the rhamnolipid mixture produced. The rhamnolipid mixture exhibited a strong surface activity by lowering the surface tension of distilled water to 28.6 mN/m with a CMC value of 195 mg/l.

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Acknowledgments

This work was supported by the 863 Program (Grant No. 2013AA064403) and the National Natural Science Foundation of China (Grant No. 41273084).

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Authors and Affiliations

  1. State Key Lab of Bioreactor Engineering, East China University of Science and Technology, Shanghai, 200237, China

    Jin-Feng Liu, Shi-Zhong Yang & Bo-Zhong Mu

  2. Petroleum Production Engineering Research Institute of Huabei Oilfield Company, CNPC, Renqiu, 062552, Hebei Province, China

    Gang Wu

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  1. Jin-Feng Liu
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Correspondence to Bo-Zhong Mu.

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Liu, JF., Wu, G., Yang, SZ. et al. Structural characterization of rhamnolipid produced by Pseudonomas aeruginosa strain FIN2 isolated from oil reservoir water. World J Microbiol Biotechnol 30, 1473–1484 (2014). https://doi.org/10.1007/s11274-013-1565-0

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  • DOI: https://doi.org/10.1007/s11274-013-1565-0

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