Abstract
Drought is an abiotic stress that limits plant growth and productivity, and the development of trees with improved drought tolerance is expected to expand potential plantation areas and to promote sustainable development. Previously we reported that transgenic poplars (Populus tremula × P. tremuloides, T89) harboring the stress-responsive galactinol synthase gene, AtGolS2, derived from Arabidopsis thaliana were developed and showed improved drought stress tolerance in laboratory conditions. Herein we report a field trial evaluation of the AtGolS2-transgenic poplars. The rainfall-restricted treatments on the poplars started in late May 2020, 18 months after transplanting to the field, and were performed for 100 days. During these treatments, the leaf injury levels were observed by measuring photosynthetic quantum yields twice a week. Observed leaf injury levels varied in response to soil moisture fluctuation and showed a large difference between transgenic and non-transgenic poplars during the last month. Comparison of the leaf injury levels against three stress classes clustered by the machine learning approach revealed that the transgenic poplars exhibited significant alleviation of leaf injuries in the most severe stress class. The transgenes and transcript levels were stable in the transgenic poplars cultivated in the field conditions. These results indicated that the overexpression of AtGolS2 significantly improved the drought stress tolerance of transgenic poplars not only in the laboratory but also in the field. In future studies, molecular breeding using AtGolS2 will be an effective method for developing practical drought-tolerant forest trees.
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Abbreviations
- GolS2:
-
Galactinol synthase 2
- RFO:
-
Raffinose family oligosaccharide
- QY:
-
Quantum yield
- ANOVA:
-
Analysis of variant
- HSD:
-
Honestly significant difference
- GMM:
-
Gaussian mixture model
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Acknowledgements
We thank Ms. Kazue Mase (Univ. Tsukuba), Ms. Akiko Sato and Ms. Kayo Kitaura (RIKEN, Japan), and Ms. Ayumi Ihara and Ms. Yuki Mitsubayashi (NAIST, Japan) for their excellent technical supports. This work was supported in part by a grant from the Center for Sustainable Resource Science, RIKEN, Japan (TO, MO, TD, and KNW), and by Cooperative Research Grants of the Plant Transgenic Design Initiative Program, Gene Research Center, University of Tsukuba (Grant nos. 1524, 1828, 1928, 2028, and 2140) (TO, MO, and TD).
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Shikakura, Y., Oguchi, T., Yu, X. et al. Transgenic poplar trees overexpressing AtGolS2, a stress-responsive galactinol synthase gene derived from Arabidopsis thaliana, improved drought tolerance in a confined field. Transgenic Res 31, 579–591 (2022). https://doi.org/10.1007/s11248-022-00321-x
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DOI: https://doi.org/10.1007/s11248-022-00321-x